Scopus İndeksli Yayınlar Koleksiyonu
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Article Visual Tracking Efficiency across Different Spacing Widths: A Text-Free Eye-Tracking Study(Routledge Journals, Taylor & Francis Ltd, 2026) Akyurek, Gokcen; Akcay, Arif; Aydoner Bektas, SelenThis study examined how spatial spacing influences visual tracking efficiency using a novel text-free eye-tracking paradigm designed to minimize the influence of reading-related processes. Twenty participants visually followed straight, curved, and angular paths with five different widths (0.5-3 cm), while eye movements were recorded using a Tobii Eye Tracker T120. Results showed that narrow angular paths (0.5-1 cm) were associated with significantly higher fixation counts and longer fixation durations compared to wider angular paths and other paths. For angular paths, fixation counts differed significantly between 0.5 cm and 2.5 cm (p = .005), and between 0.5 cm and 3 cm (p = .003). Total fixation counts and total fixation durations were significantly greater for angular paths than for straight and curved paths. The findings indicate that narrower spatial constraints increase visual-perceptual load and reduce oculomotor fluency, whereas moderate spacing (approximately 2.5 cm) facilitates smoother visual guidance. The results are limited to visual tracking and oculomotor control within constrained geometric layouts and do not reflect reading behavior. The study highlights the utility of text-free visual tracking tasks for isolating low-level perceptual and oculomotor processes and informing future research on spatial layout and visual guidance in digital environments.Article Women’s Biopsychosocial Experiences Following Embryo Transfer: A Qualitative Study(Elsevier Masson, Corp Off, 2026) Baltacı, Nurşah Boylu; Aslan, Ergül; Coşkun, MervePurpose: This study explored the physical, psychological, and social experiences of women during the waiting period following embryo transfer (ET) in In Vitro Fertilization (IVF) and provides recommendations regarding psychosocial support needs. Materials and methods: A descriptive phenomenological design with thematic analysis was used. Semi-structured telephone interviews (30-45 min) were conducted with 29 women who underwent ET at a private IVF center in Istanbul from April to July 2025. Data were coded and analyzed using MAXQDA 24, and themes and subthemes were identified. Results: Participants had a mean age of 32.8 years, infertility duration of 2.8 years, 2.2 of IVF attempts, and 2.5 ETs. Most women were employed (69%), 72% had no previous pregnancies, and 86% had undergone multiple IVF attempt. Four main themes emerged in the thematic analysis: physical effects, psychological effects, social effects, and coping strategies. Subthemes varied by marital duration, previous IVF experience, and number of ETs; effects were stronger among women with multiple ETs. Conclusion: Women experience multidimensional challenges following ET and adopt various coping strategies. Physical changes disrupt daily life, emotional fluctuations stem from uncertainty and anxiety, and partner/social support and trust in healthcare professionals are critical for mitigating anxiety and improving quality of life and treatment adherence.Article Virtual Reality-Based Rehabilitation for Upper Extremity Recovery after Stroke: A Systematic Review and Meta-Analysis of Randomized Controlled Trials(Taylor & Francis Ltd, 2026) Ozturk, BasarBackground: Stroke often results in upper extremity motor impairments, limiting functional independence. Conventional rehabilitation, while effective, faces challenges such as low engagement and high resource demand. Virtual reality (VR)-based rehabilitation may promote neuroplasticity through immersive and repetitive training. Objectives: To systematically evaluate randomized controlled trials (RCTs) assessing the effectiveness of VR-based rehabilitation on upper extremity motor recovery in stroke patients, and to explore the impact of system type, stroke chronicity, and intervention dosage. Methods: Following PRISMA 2020 guidelines (PROSPERO: CRD420251065021), a systematic search was conducted in PubMed, Scopus, Web of Science, and CENTRAL for RCTs published between 2010 and March 2025. Studies included adult stroke survivors receiving VR interventions targeting upper limb recovery. A random-effects meta-analysis calculated standardized mean differences (SMDs), and risk of bias was assessed using RoB 2.0. Results: Twenty-four RCTs (n = 1,627) were included. Meta-analysis of 21 studies showed a moderate effect favoring VR (SMD = 0.58; 95% CI: 0.38-0.78; p < 0.001; I-2 = 62%). Immersive VR, chronic stroke, and higher intervention doses (>600 min) showed stronger effects. Conclusions: VR-based rehabilitation significantly enhances upper extremity motor function post-stroke. Immersive systems and sufficient dosage yield optimal outcomes, supporting VR as a valuable adjunct to conventional therapy.Article Turkish Translation and Cultural Adaptation of the Test of Ideational Praxis(SAGE Publications Ltd, 2026) Asqarova, Sevda; May-Benson, Teresa; Sagliyan, Mustafa; Balikci, Aymen; Sirma, Gamze CaglaIntroduction: The Test of Ideational Praxis is a performance-based assessment designed to identify ideational praxis difficulties in children aged 3-12. However, Turkish therapists are currently unable to use this tool due to the lack of a linguistically and culturally appropriate version. This study aimed to develop a culturally adapted Turkish version of the Test of Ideational Praxis to support future normative data collection, psychometric evaluations and clinical application.Method: A seven-step process for translation and cross-cultural adaptation was conducted, including forward and backwards translation, expert synthesis, linguistic review, structured therapist evaluations and pre-testing with children. Each step was guided by established literature and best practice guidelines.Findings: The translation and adaptation process was completed successfully with 29 participants, including translators, therapists and 10 typically developing children aged 6-8 years (four girls and six boys). While minor revisions were required during the synthesis and linguistic review phases, the forward translations showed strong semantic and conceptual consistency. Discrepancies observed in the back translation were limited to phrasing and did not affect standardised administration or scoring. Both therapists and children confirmed that the Turkish version was clear, comprehensible and culturally appropriate, with no expressions, instructions or symbolic representations perceived as problematic.Conclusion: The Turkish translation and adaptation of the Test of Ideational Praxis provides a culturally adapted instrument that supports future validation studies and clinical piloting, offering a foundation for both research and practice on ideational praxis development in Türkiye.Article The Practice Environment Scale of the Nursing Work Index Short Form in Turkish: A Psychometric Study(BMC, 2026) Arici, Furkan Cihat; Harmancı Seren, Arzu Kader; Seren, Arzu Kader Harmanci; Ayan, GüzinBackgroundThe work environment is a concept encompassing factors that increase or decrease a nurse's ability to practice the nursing profession competently and provide high-quality care. This study aims to adapt the short form of the Nursing Work Index-Nursing Work Environment Assessment Scale, which is used in many countries to evaluate the nursing work environment, to Turkish.MethodsThe study sample consisted of 711 nurses. For this study, data were collected from the researchers' personal social media accounts (WhatsApp, LinkedIn, and Instagram) between October and December of 2024. The Turkish version of the short form of the Practice Environment Scale from the Nursing Work Environment Index was used to collect the data. After creating the Turkish version of the instrument through translation and back-translation and collecting data, the researchers conducted content validity analyses, construct validity tests, and internal consistency analyses to assess the instrument's psychometric properties.ResultsThe items' content validity ratios were 0.93, and correlation coefficients varied between 0.605 and 0.523. The one structure accounted for 56.122% of the total variance. PES-5 scale findings: chi & sup2;/df 3.76, CFI 0.973, TLI 0.946, SRMR 0.031, RMSEA 0.090, CR 0.62. The standardized factor loadings for the scale items ranged from 0.610 to 0.743. The scale was compatible with its five items and one-factor structure The items' standardized factor loadings ranged from 0.370 to 0.575. The Cronbach's alpha consistency value was 0.804.ConclusionA short form of the practice environment scale of the Nursing Work Index is a valid and reliable tool in Turkish.Book Part The Genetics of Norovirus: Understanding Its Genome and Genogroups(Wiley, 2027) Sharma, Rahul; Kagithala, Naga Rani; Kothamasu, Sasikanth; Srivastava, Shriyansh; Ibrahim, Ismail A.Article Synergistic Anticancer Effects of the PLK1 Inhibitor BI-2536 and β-Glucan in Colon and Gastric Cancer Cells(International Institute of Anticancer Research, 2026) Saraç, Bülent; Joha, Ziad; Takci, Rabia Gökçe; Hacisüleyman, Levent; Şahİnbaş, BurcuArticle Sub-Cytotoxic Mitochondrial Stress in Cardiomyocytes and Whole-Organism Toxicity in C. Elegans Induced by Molnupiravir(SAGE Publications Inc, 2026) Gunaydin-Akyildiz, Aysenur; Keskin, Zehra; Ozhan, Gul; Kuran, Ebru Didem; Gulec, MeltemMany antiviral agents are known to induce off-target mitochondrial toxicity due to the prokaryotic origin of mitochondria. Mitochondrial dysfunction is frequently linked to cardiotoxicity. We aimed to elucidate the mitochondrial toxicity profile of molnupiravir via focusing on mitochondrial dynamics, biogenesis, and oxidative stress in cardiac cells. Mitochondrial function was evaluated by luminometric measurement of ATP (adenosine triphosphate) content, and flow cytometric analysis of mitochondrial membrane potential, and mitochondrial mass. The expression levels of genes involved in mitochondrial fusion-fission were assessed by RT-PCR. In addition, molecular docking analysis was performed to evaluate the interaction between molnupiravir and the dynamin related-protein DRP1. Protein carbonylation was determined as an oxidative stress parameter. Toxicity evaluation was further investigated in Caenorhabditis elegans to support the in vitro findings at the organismal level. Molnupiravir exposure led to a significant dose-dependent reduction in intracellular ATP level and mitochondrial mass, accompanied by increased protein carbonylation. Mitochondrial membrane potential remained slightly increased. Alterations in the expression of genes regulating mitochondrial dynamics suggested an imbalance between fusion and fission processes, while mitochondrial biogenesis-related signaling was progressively suppressed. C. elegans exposed to higher concentrations of the drug (20-500 & micro;M) exhibited significant lifespan reduction at all doses. Molecular docking analysis demonstrated a moderate binding affinity of molnupiravir to DRP1, supporting a potential direct interaction with mitochondrial fission machinery. In conclusion, our results demonstrate that molnupiravir induces mitochondrial stress through oxidative damage, impaired biogenesis, and altered dynamics, emphasizing the need for careful evaluation of mitochondrial safety of molnupiravir in cardiac tissue.Article Telehealth Delivery of the Homeostasis–Enrichment–Plasticity Approach for Premature Infants With Developmental Risks: Exploratory Feasibility Study(JMIR Publications, Inc, 2026) May-Benson, Teresa A.; Demirbag, Izgi-Miray; Sirma, Gamze Cagla; Sozen, Hatice Gulhan; Aracikul Balikci, Ayse Firdevs; Ilbay, Gul; Balikci, AymenBackground: Preterm delivery is an increasing worldwide health concern linked to increased neurodevelopmental risks. Early intervention is crucial for harnessing neuroplasticity to enhance developmental and functional performance outcomes; however, access to early intervention is frequently hindered by logistical, financial, and labor constraints. The Homeostasis-Enrichment-Plasticity (HEP) Approach is a family-centered early intervention model based on enriched environments, designed to improve infants' sensory-motor, cognitive, and socio-emotional development. Objective: This study aimed to assess the feasibility, safety, acceptability, and outcomessensitivity to change of implementing the HEP Approach through telehealth for premature infants at developmental risk. Methods: A pre-post exploratory feasibility study was performed, including 16 preterm infants (aged 4-12 months corrected age), of whom 14 completed the study. The 12-week intervention included weekly remote sessions focused on environmental enrichment, active exploration, and parental guidance. Thefeasibility and acceptability were evaluated using a 24-item questionnaire. Developmental outcomeswere assessed with the Young Children's Participation and Environment Measure, Ages and Stages Questionnaire (ASQ), Alberta Infant Motor Scale, Infant Motor Profile, and Depression Anxiety Stress Scales. Results: High adherence (14/14, 100%) and retention (14/16, 87.5%) rates demonstrated robust feasibility. Parents indicated 86%-100% agreement across all feasible criteria, affirming safety, satisfaction, and acceptability. No adverse incidents were reported. Changes were identified in participation (Young Children's Participation and Environment Measure), motor development (Alberta Infant Motor Scale, Infant Motor Profile, andASQ), communication and social-emotional domains (ASQ), and caregiver well-being (Depression Anxiety Stress Scales) (P<.05). Conclusions: The telehealth implementation of the HEP Approach demonstrated feasibility, safety, and strong acceptance among families, along with quantifiable developmental and psychosocial changes. These initial findings endorse the model's viability as an accessible, family-oriented telehealth framework for infants born preterm. Future randomized controlled and longitudinal studies are necessary to validate intervention efficacy and scalability.Article Synthesis, Experimental and Quantum Mechanical Investigation of the Crystal Structures of Two Triazolyl-Indole Compounds, along with the Evaluation of Their Antioxidant Activity(Elsevier, 2026) Pınar, Deniz; Kurt-Şirin, Özlem; Ulusoy-Güzeldemirci, Nuray; Dincel, Efe Doğukan; Karayel, Arzu; Kuran, Ebru DidemIn this study, two 1,2,4-triazolyl-indole compounds, 5-(1H-indol-3-yl)-4-propyl-2,4 dihydro-3H-1,2,4-triazole-3-thione (3a) and 5-(1H-indol-3-yl)-4-butyl-2,4-dihydro-3H-1,2,4-triazole-3-thione (3b), were synthesized and the structures of these compounds were determined by X-ray diffraction method. These two compounds crystallize in the orthorhombic space groups, Pbca. The structures have been determined by direct methods and refined to R, 0.0459 (3a) and 0.0714 (3b). The molecular and crystal structures are stabilized by two intermolecular hydrogen bond for both molecules. The triazole parts of the molecules are almost perpendicular to propyl and butyl groups. NMR studies was also performed to enhance comprehension of the molecular structure of the compounds. The most stable states of the structures, as determined by both DFT analysis and experimental realizations (X-ray, NMR and FT-IR), are the thione form. Although the thione forms are thermodynamically more stable than the thiol forms (Delta H degrees approximate to-15 kcal/mol), they display higher chemical reactivity, as reflected by their smaller HOMO-LUMO energy gaps (Delta E = 4.374 eV for 3a and 4.377 eV for 3b). In addition, the antioxidant activities of both compounds were evaluated using ABTS, DPPH, and FRAP assays. Although both (3a) and (3b) exhibited measurable radical scavenging and reducing power, their antioxidant capacities were found to be lower than that of the reference compound quercetin.Erratum Retraction Notice to “Plantar Pressure Reconfiguration via Custom CAD/CAM Insoles in Pes Planus: A Pedobarographic and PCA-Based Study”(Elsevier B.V., 2026) Eroğlu, Muhammet; Öztürk, Başar; Naci, Baha; Bulut, Zeynep İrem; Bahadır, Berkan; Ataç, EmreArticle Shoulder Muscular Activation during Common Kettlebell Exercises: A Randomized Cross-Sectional Surface EMG Study(Taylor & Francis Inc, 2026) Paköz, Büşra; Işiklar, Çağdaş; Turgut, ElifPurpose This study aimed to examine shoulder muscle activation patterns within functionally similar upper-body exercise categories performed using different resistance modalities. Methods Twenty healthy males (mean age 22.6 +/- 1.69 years) performed overhead pressing, rowing, and swinging tasks using kettlebells, dumbbells, and elastic bands. Surface electromyography (EMG) was used to record activity from seven shoulder muscles. The recorded electromyographic signals were normalized to maximal voluntary isometric contractions (%MVIC), log-transformed, and analyzed using a two-way repeated-measures analysis of variance (ANOVA) with exercise and muscle as within-subject factors. Results A repeated-measures ANOVA on log-transformed EMG data showed significant main effects of exercise (F(2.10, 39.95)=22.04, p<.001, eta(2) p=.537) and muscle (F(4.49, 85.34)=47.55, p<.001, eta(2) p=.714), as well as a significant exercise & times;muscle interaction (F(8.16, 154.98)=82.84, p<.001, eta(2) p=.813). Within the overhead pressing category, the Bottoms-Up Kettlebell Overhead Press elicited significantly greater activation than the traditional Kettlebell Overhead Press in the upper trapezius, lower trapezius, biceps brachii, and infraspinatus (all p<.050), with upper trapezius activation reaching 30.4 +/- 13.4%MVIC. In the rowing category, middle and lower trapezius activation was greater during free-weight rowing than during elastic band rowing, while serratus anterior activation remained relatively low across the rowing conditions. In the swing category, activation patterns were largely comparable between the two-hand kettlebell and dumbbell swings, whereas the one-hand kettlebell swing produced greater activation in selected muscles, with the lower trapezius showing the highest activation. Absolute between-exercise differences in %MVIC were modest. Conclusion Kettlebell, dumbbell, and elastic band exercises elicit distinct shoulder muscle activation profiles. These findings may inform exercise selection and program design when targeting specific shoulder muscles within task-oriented training contexts.Article Selective Recovery and Preconcentration of Catechins from Wine Industry Residues Using Molecularly Imprinted Polymers: An Eco-Friendly Solid Phase Extraction Approach(Springer, 2026) Özen, Seda; Tütem, Esma; Ersoy, Şeyda KaramanMolecularly imprinted polymers (MIPs) are engineered materials that provide exceptional selectivity and stability for target molecule recognition. While most previous catechin-imprinted polymer (CAT-MIP) studies have focused on tea matrices, the present study applies CAT-MIP technology to wine industry residues, particularly grape seeds, which represent sustainable and underutilized sources of bioactive compounds. CAT-MIP was synthesized using CAT as the template molecule and polymerization parameters including functional monomer type, porogen composition, and template-to-monomer-to-crosslinker molar ratios were systematically optimized. Among the tested conditions, 4-vinylpyridine (4-VP) and an acetonitrile: dimethyl sulfoxide (ACN: DMSO, 98:2, v/v) solvent system provided the most favorable imprinting performance. Adsorption studies revealed heterogeneous binding behavior consistent with the Freundlich model, while Langmuir parameters were used for comparative evaluation. The optimized CAT-MIP was successfully applied as a molecularly imprinted solid-phase extraction (MISPE) sorbent, achieving selective recovery of 69.20 +/- 2.45% for CAT and 52.50 +/- 1.77% for epicatechin (ECAT) from white grape seed extracts, with comparable performance observed for red grape seed extracts. This MISPE based approach offers the first comprehensive study applying CAT-MIP technology to wine industry waste valorization, comparing red and white grape varieties, and establishing a fully validated HPLC-PDA (high performance liquid chromatography-photodiode array detection) method for CAT quantification. The eco-friendly approach addresses the growing need for sustainable extraction of valuable high-value natural antioxidants. from agro-industrial byproducts.Article Protective Effects of Panax Ginseng against Bisphenol A-Induced Testicular Toxicity in Rats(Springer Nature, 2026) Cesur, Yasemin; Cam, Kamil; Pazarbasi, Seren Ede; Dorucu, Dogancan; Sener, Goksel; Abas, Burcin Irem; Cevik, OzgeBisphenol A (BPA) is an endocrine-disrupting chemical known to cause testicular toxicity through oxidative stress and apoptosis. Panax ginseng (PG) is a natural product with anti-inflammatory effects. This study aimed to evaluate the protective effect of PG against BPA-induced testicular damage in rats. Thirty-two Wistar Albino rats aged 10-12 weeks were divided into four groups: Control, PG, BPA, and BPA + PG. BPA (50 mg/kg/day) and PG (100 mg/kg/day) were orally administered for 6 weeks. BPA significantly increased serum total oxidant status and decreased antioxidant status (p < 0.001, p < 0.001). Also, the levels of superoxide dismutase (an antioxidant enzyme) (p = 0.0005) and androgen receptor-mRNA (an androgen signaling marker) decreased (p = 0.014). However, caspase 3 (an apoptosis marker) (p = 0.0067), 8-hydroxy-2'-deoxyguanosine (a marker of oxidative DNA damage) (p < 0.001), and tumor necrosis factor-alpha (a proinflammatory cytokine) (p < 0.001) levels increased in testicular tissues. Rats treated with PG showed improvements in all oxidative and inflammatory markers and significantly restored androgen receptor expression. Histopathological examination revealed degeneration in seminiferous tubules and reduced spermatozoa in the BPA group, while the BPA + PG group showed marked improvement. These findings suggest that PG may alleviate oxidative stress-related testicular damage at both molecular and histological levels and may offer insights for future clinical studies.Article Novel Hydrazide-Hydrazone Derivatives Containing Flurbiprofen 1,2,4-Triazole as Anticancer Agents: Design, Synthesis and Biological Evaluation(Slovensko Kemijsko Drustvo, 2026) Küçükgüzel, Ş. Güziz; Gökoğlan, Ecem; Yılmaz, Özgür; Çevik, Özge; Çakıcı, Çiğdem; Erdoğan, ÖmerHydrazone derivatives are one of the scaffolds frequently used in new drug development studies. Due to the promising pharmacological effects of the hydrazone structure, fifteen new hydrazide-hydrazone compounds containing flurbiprofen 1,2,4-triazole were synthesized in this study and their in vitro anticancer effects were tested. All compounds were tested for cytotoxic effects against breast cancer cell lines (MCF-7 and MDA-MB231), and glioblastoma cell line (U87) by using MTT assay. Among the synthesized compounds, compounds 7a and 7c exhibited the most potent cytotoxic activity with IC50 values of 7.80 +/- 1.20 & micro;M and 2.40 +/- 0.93 & micro;M against MCF-7 cell line, while compound 7a showed the highest activity with IC50 value of 7.63 +/- 1.05 & micro;M against MDA-MB231 cell line. In addition, compounds 7c and 7n presented cytotoxic activity with IC50 values of 10.31 +/- 4.63 & micro;M and 10.81 +/- 6.11 & micro;M against U87 cell line. The possible cytotoxic effects of compounds on mouse fibroblast cell line (L929) were assessed for their safety and compounds 7a, 7c, and 7n were found less toxic than 5-fluorourasil. Additionally, compound 7c was further studied to investigate its effects on apoptosis and PI3K activity, which play a role in cancer development. The results showed that compound 7c increased apoptosis in MCF-7 cells and it displayed PI3K enzyme inhibitory activity. Our study revealed that the synthesized hydrazone compounds have the potential to be lead compounds for further studies on cancer.Article Prevalence and Risk of Carpal Tunnel Syndrome in Parkinson’s Disease: A Systematic Review and Meta-Analysis(MDPI, 2026) Raafat, Kareem Wael; Amin, Ahmed M.; Ezz, Mohamed R.; Sabry, Ehab Naser; Ibrahim, Ismail A.; Attia, Amir N.; Mohammed, Mariam M.Background: Parkinson's disease (PD) is a progressive neurodegenerative disorder characterised by motor and non-motor symptoms. Several studies have reported varying prevalence of Carpal Tunnel Syndrome (CTS) among individuals with PD. Objective: This study aimed to estimate the pooled prevalence of CTS in people with PD and explore any potential association between the two conditions. Methods: This systematic review and meta-analysis was conducted and reported in accordance with the PRISMA 2020 guidelines. A systematic search was performed across PubMed, the Cochrane Central Register of Controlled Trials (CENTRAL), Web of Science (WoS), Scopus, and EMBASE from inception to April 2024. Studies reporting CTS prevalence data in individuals with PD were included. Methodological quality was assessed using the National Institutes of Health (NIH) quality assessment tool. Pooled prevalence estimates were calculated using a random-effects model. Risk difference (RD) and risk ratio (RR) were calculated to assess the association between PD and CTS compared with control groups. Results: A total of 7 studies involving 411 participants (343 with PD and 68 controls) met the inclusion criteria, with 679 wrists assessed. The pooled prevalence of CTS in PD was estimated at 15% (95% CI: 0.07-0.28) with significant heterogeneity (p < 0.001, I-2 = 91%). The RD was 10% (95% CI: 0.04-0.16, p = 0.002), with low heterogeneity (p = 0.29, I-2 = 19%). The RR of CTS in PD compared with controls was 3.31 (95% CI: 0.60-18.42, p = 0.17), with moderate heterogeneity (p = 0.13, I-2 = 52%). Conclusions: This meta-analysis provides preliminary pooled estimates indicating a potentially increased prevalence of carpal tunnel syndrome in individuals with PD. Although the findings suggest a possible association, clinicians should maintain increased vigilance for CTS symptoms in patients with PD presenting with upper-limb sensory or motor complaints. From a biomechanical and functional perspective, these findings highlight the importance of routine upper-limb screening and the implementation of rehabilitation strategies targeting hand use, dexterity, and sensorimotor control within physiotherapy practice. Further high-quality studies with larger, well-characterised samples are required to confirm this relationship and clarify its clinical and functional implications.Article Phthalocyanine@MXene Based Hybrid Composite Materials for Flexible Supercapacitor Applications(Elsevier B.V., 2026) Kayir, Nisa; Unal, Bayram; Sahin, Yucel; Gorduk, Semih; Artar, Emirhan; Gorduk, OzgeArticle Metabolic Responses to Benzoic Acid Stress and Glutamine Transport-Dependent Vulnerabilities in Escherichia Coli Revealed by NMR Metabolomics(Springer, 2026) Yuksektepe, Ecem; Elgin, Emine Sonay; Onat-Tasdelen, Kadriye Aslihan; Chae, Young Kee; Dogu, Eralp; Catav, Sukru Serter; Ozturkel-Kabakas, HaticeBenzoic acid (BA) is a widely used weak organic acid preservative with antimicrobial activity, yet the metabolic basis of its antibacterial action and the determinants of bacterial sensitivity remain incompletely understood. Here we combined growth assays with H-1 NMR metabolomics to characterize BA-induced metabolic responses in Escherichia coli BW25113 and to examine metabolic changes associated with impaired glutamine transport. Wild-type BW25113 and its BA-sensitive isogenic Delta glnP mutant, lacking the membrane-bound glutamine permease of the high-affinity GlnHPQ transport system, were exposed to sublethal BA concentrations. BA slowed growth and significantly altered the levels of 42 metabolites in the wild-type and 38 in Delta glnP, with the mutant showing stronger growth inhibition and reduced BA tolerance. Both strains exhibited metabolic changes consistent with cellular responses to oxidative and acid stress, including alterations in central carbon metabolism, lysine degradation, cysteine and methionine metabolism, pyrimidine metabolism, and one-carbon pool by folate. However, several metabolic responses differed between the two strains. In wild-type cells, BA exposure was associated with changes in glycerolipid metabolism, glycerophospholipid metabolism, nicotinate and nicotinamide metabolism, lysine biosynthesis, glycine, serine and threonine metabolism, and purine metabolism. In contrast, Delta glnP cells showed distinct alterations in D-amino acid metabolism, arginine biosynthesis, and other carbon fixation pathways. In addition, the mutant displayed substantial baseline differences relative to the wild-type, including altered nucleotide and amino acid pools. Together, these results indicate that both BA exposure and deletion of glnP induce broad metabolic adjustments in Escherichia coli. Loss of glnP is associated with distinct metabolic states and altered responses to BA stress, highlighting the importance of glutamine transport in adaptation to weak organic acid stress.Conference Object Low Error-Rate Temperature Prediction with Machine Learning Using LoRaWAN Data(Institute of Electrical and Electronics Engineers Inc., 2025) Nas, Abdurrahim; Yildiz, Omer; Karlik, Sait EserBook Part Neuroscientific Approach to Artificial Intelligence for Psychopathologies(IGI Global, 2026) Celik, Kübra; Celik, Samet
