Functional Groups Matter: Metabolomics Analysis of <i>escherichia Coli</I> Exposed To Trans-Cinnamic Acid and Its Derivatives Unveils Common and Unique Targets

dc.authorid Catav, Sukru Serter/0000-0002-9934-254X
dc.authorid Elgin, Emine Sonay/0000-0002-1278-328X
dc.authorscopusid 58771273400
dc.authorscopusid 58771286500
dc.authorscopusid 57224639301
dc.authorscopusid 55336502000
dc.authorscopusid 58771260800
dc.authorscopusid 24174496800
dc.authorscopusid 55665275900
dc.authorwosid Elgin, Emine/JTT-6369-2023
dc.authorwosid Catav, Sukru Serter/R-6087-2017
dc.contributor.author Yüksektepe, Ecem
dc.contributor.author Ozturkel-Kabakas, Hatice
dc.contributor.author Yuksektepe, Ecem
dc.contributor.author Catav, Sukru Serter
dc.contributor.author Guzel, Gulnur
dc.contributor.author Col, Bekir
dc.contributor.author Elgin, Emine Sonay
dc.contributor.other Tıbbi Hizmetler ve Teknikler Bölümü
dc.date.accessioned 2025-01-11T13:03:14Z
dc.date.available 2025-01-11T13:03:14Z
dc.date.issued 2024
dc.department Fenerbahçe University en_US
dc.department-temp [Onat-Tasdelen, Kadriye Aslihan; Ozturkel-Kabakas, Hatice] Mugla Sıtkı Kocman Univ, Grad Sch Nat & Appl Sci, Biol Program, Mugla, Turkiye; [Yuksektepe, Ecem] Fenerbahce Univ, Vocat Sch Hlth Serv, Pathol Lab Tech Program, Istanbul, Turkiye; [Catav, Sukru Serter; Col, Bekir] Mugla Sıtkı Kocman Univ, Coll Sci, Dept Biol, Mugla, Turkiye; [Guzel, Gulnur] Mugla Sıtkı Kocman Univ, Grad Sch Nat & Appl Sci, Chem Program, Mugla, Turkiye; [Kim, Hakbeom; Chae, Young Kee] Sejong Univ, Coll Nat Sci, Dept Chem, Seoul, South Korea; [Elgin, Emine Sonay] Mugla Sıtkı Kocman Univ, Coll Sci, Dept Chem, Mugla, Turkiye; [Col, Bekir] Mugla Sıtkı Kocman Univ, Biotechnol Res Ctr, Mugla, Turkiye; [Elgin, Emine Sonay] Mugla Sıtkı Kocman Univ, Res Labs Ctr, Metab Lab, Mugla, Turkiye en_US
dc.description Catav, Sukru Serter/0000-0002-9934-254X; Elgin, Emine Sonay/0000-0002-1278-328X en_US
dc.description.abstract Phenolic acids are derivatives of benzoic and cinnamic acids, which possess important biological activities at certain concentrations. Trans-cinnamic acid (t-CA) and its derivatives, such as p-coumaric acid (p-CA) and ferulic acid (FA) have been shown to have antibacterial activity against various Gram-positive and -negative bacteria. However, there is limited information available concerning the antibacterial mode of action of these phenolic acids. In this study, we aimed to ascertain metabolic alterations associated with exposure to t-CA, p-CA, and FA in Escherichia coli BW25113 using a nuclear magnetic resonance (NMR)-based metabolomics approach. The results showed that t-CA, p-CA, and FA treatments led to significant changes (p < 0.05) in the concentration of 42, 55, and 74% of the identified metabolites in E. coli, respectively. Partial least-squares discriminant analysis (PLS-DA) revealed a clear separation between control and phenolic acid groups with regard to metabolic response. Moreover, it was found that FA and p-CA treatment groups were clustered closely together but separated from the t-CA treatment group. Arginine, putrescine, cadaverine, galactose, and sucrose had the greatest impact on group differentiation. Quantitative pathway analysis demonstrated that arginine and proline, pyrimidine, glutathione, and galactose metabolisms, as well as aminoacyl-tRNA and arginine biosyntheses, were markedly affected by all phenolic acids. Finally, the H2O2 content of E. coli cells was significantly increased in response to t-CA and p-CA whereas all phenolic acids caused a dramatic increase in the number of apurinic/apyrimidinic sites. Overall, this study suggests that the metabolic response of E. coli cells to t-CA is relatively different from that to p-CA and FA. However, all phenolic acids had a certain impact on oxidative/antioxidant status, genomic stability, arginine-related pathways, and nucleic acid metabolism. en_US
dc.description.sponsorship Trkiye Bilimsel ve Teknolojik Arascedil;timath;rma Kurumu en_US
dc.description.sponsorship No Statement Available en_US
dc.description.woscitationindex Science Citation Index Expanded
dc.identifier.citation 1
dc.identifier.doi 10.1007/s11274-023-03841-8
dc.identifier.issn 0959-3993
dc.identifier.issn 1573-0972
dc.identifier.issue 2 en_US
dc.identifier.pmid 38114822
dc.identifier.scopus 2-s2.0-85180133456
dc.identifier.scopusquality Q2
dc.identifier.uri https://doi.org/10.1007/s11274-023-03841-8
dc.identifier.uri https://hdl.handle.net/20.500.14627/242
dc.identifier.volume 40 en_US
dc.identifier.wos WOS:001126500600002
dc.identifier.wosquality Q2
dc.language.iso en en_US
dc.publisher Springer en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/closedAccess en_US
dc.scopus.citedbyCount 5
dc.subject Antibacterial Response en_US
dc.subject Escherichia Coli en_US
dc.subject Ferulic Acid en_US
dc.subject Metabolomics en_US
dc.subject P-Coumaric Acid en_US
dc.subject Trans-Cinnamic Acid en_US
dc.title Functional Groups Matter: Metabolomics Analysis of <i>escherichia Coli</I> Exposed To Trans-Cinnamic Acid and Its Derivatives Unveils Common and Unique Targets en_US
dc.type Article en_US
dc.wos.citedbyCount 5
dspace.entity.type Publication
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