The Effect of Whey Proteins on the Brain and Small Intestine Nitric Oxide Levels: Protein Profiles in Methotrexate-Induced Oxidative Stress

dc.authorid DURSUN, Ercan/0000-0001-6025-9565
dc.authorid Sener, Goksel/0000-0001-7444-6193
dc.authorid Tufan, Elif/0000-0003-0684-3693
dc.authorscopusid 58643197000
dc.authorscopusid 57394511800
dc.authorscopusid 57394797300
dc.authorscopusid 57203904440
dc.authorscopusid 58174308800
dc.authorscopusid 6504603410
dc.authorscopusid 55662834600
dc.authorwosid Dursun, Ercan/JXN-9633-2024
dc.contributor.author Yılmaz Karaoğlu, Sümeyye
dc.contributor.author Tufan, Elif
dc.contributor.author Sivas, Guzin Goksun
dc.contributor.author Gokmen, Begum Gurel
dc.contributor.author Dursun, Ercan
dc.contributor.author Ozbeyli, Dilek
dc.contributor.author Tunali-Akbay, Tugba
dc.contributor.other Eczane Hizmetleri Bölümü
dc.date.accessioned 2025-01-11T13:01:47Z
dc.date.available 2025-01-11T13:01:47Z
dc.date.issued 2022
dc.department Fenerbahçe University en_US
dc.department-temp [Yilmaz, Sumeyye; Sener, Goksel] Fenerbahce Univ, Vocat Sch Hlth Serv, Istanbul, Turkey; [Tufan, Elif; Sivas, Guzin Goksun; Gokmen, Begum Gurel; Dursun, Ercan; Tunali-Akbay, Tugba] Marmara Univ, Fac Dent, Dept Basic Med Sci, Istanbul, Turkey; [Ozbeyli, Dilek] Marmara Univ, Vocat Sch Hlth Serv, Istanbul, Turkey en_US
dc.description DURSUN, Ercan/0000-0001-6025-9565; Sener, Goksel/0000-0001-7444-6193; Tufan, Elif/0000-0003-0684-3693 en_US
dc.description.abstract Objectives: The aim of this study was to determine the effects of whey proteins on methotrexate (MTX)-induced brain and small intestine damage. Materials and Methods: 30 Sprague Dawley rats (200-300 g) were divided into four groups: Control, control + whey, MTX, and MTX+whey. MTX was administered at 20 mg/kg (single dose) intraperitoneally to the MTX group rats, and 2 mg/kg of whey protein were administered by oral gavage for 10 days to the whey groups. Lipid peroxidation, glutathione, and nitric oxide (NO) levels, as well as glutathione-Stransferase and superoxide dismutase activities were measured in the brain and small intestine. SDS-polyacrylamide gel electrophoresis of the brain and intestine tissues were also carried out. Results: While MTX treatment caused oxidative damage in the brain and small intestine, whey protein administration ameliorated MTXinduced oxidative stress. MTX administration did not change the brain's NO level, while an increase in intestinal NO level was detected. Conclusion: MTX induced oxidative stress in the brain and small intestine changed the protein metabolism in these tissues regardless of reduced food intake. Consecutive 10-day administration of whey proteins has shown its therapeutic effect on MTX-induced brain and small intestine oxidative damage. en_US
dc.description.woscitationindex Emerging Sources Citation Index
dc.identifier.citation 1
dc.identifier.doi 10.26650/experimed.1189898
dc.identifier.endpage 118 en_US
dc.identifier.issn 2667-5846
dc.identifier.issue 3 en_US
dc.identifier.scopus 2-s2.0-85173934924
dc.identifier.scopusquality Q4
dc.identifier.startpage 113 en_US
dc.identifier.trdizinid 1166380
dc.identifier.uri https://doi.org/10.26650/experimed.1189898
dc.identifier.uri https://hdl.handle.net/20.500.14627/180
dc.identifier.volume 12 en_US
dc.identifier.wos WOS:001325430100003
dc.identifier.wosquality N/A
dc.language.iso en en_US
dc.publisher Istanbul Univ en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.scopus.citedbyCount 1
dc.subject Methotrexate en_US
dc.subject Whey Protein en_US
dc.subject Brain en_US
dc.subject Small Intestine en_US
dc.subject Nitric Oxide en_US
dc.subject Oxidative Stress en_US
dc.title The Effect of Whey Proteins on the Brain and Small Intestine Nitric Oxide Levels: Protein Profiles in Methotrexate-Induced Oxidative Stress en_US
dc.type Article en_US
dc.wos.citedbyCount 0
dspace.entity.type Publication
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relation.isAuthorOfPublication.latestForDiscovery 8c521165-5eda-4dde-9444-690cc90fe1f6
relation.isOrgUnitOfPublication 133d3937-41df-4bab-8a3e-df9596f91b0e
relation.isOrgUnitOfPublication.latestForDiscovery 133d3937-41df-4bab-8a3e-df9596f91b0e

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