Morphological and Biochemical Investigation of the Protective Effects of Panax Ginseng on Methotrexate-Induced Testicular Damage

dc.authorscopusid 58513911400
dc.authorscopusid 26767983600
dc.authorscopusid 57394797300
dc.authorscopusid 24067816000
dc.authorscopusid 55662834600
dc.authorscopusid 7006979286
dc.contributor.author Karakaya-Cimen, F.B.
dc.contributor.author Macit, C.
dc.contributor.author Sivas, G.G.
dc.contributor.author Akbay, T.T.
dc.contributor.author Sener, G.
dc.contributor.author Ercan, F.
dc.date.accessioned 2025-03-10T21:19:07Z
dc.date.available 2025-03-10T21:19:07Z
dc.date.issued 2023
dc.department Fenerbahçe University en_US
dc.department-temp Karakaya-Cimen F.B., Marmara University, Institute of Health Sciences, Department of Histology and Embryology, Istanbul, Turkey, Marmara University, School of Medicine, Department of Histology and Embryology, Istanbul, Turkey, Bezmialem Vakif University, School of Medicine, Department of Histology and Embryology, Istanbul, Turkey; Macit C., Istanbul Medipol University, School of Pharmacy, Department of Pharmacology, Istanbul, Turkey; Sivas G.G., Marmara University, Faculty of Dentistry, Basic Medical Sciences, Department of Basic Health Sciences, Istanbul, Turkey; Akbay T.T., Marmara University, Faculty of Dentistry, Basic Medical Sciences, Department of Basic Health Sciences, Istanbul, Turkey; Sener G., Fenerbahce University, School of Pharmacy, Department of Pharmacology, Istanbul, Turkey; Ercan F., Marmara University, School of Medicine, Department of Histology and Embryology, Istanbul, Turkey en_US
dc.description.abstract Objective: Methotrexate (MTX) is a chemotherapeutic agent that causes testicular toxicity used in the cure of various types of cancer. The anti-oxidant and anti-cancer effects of Panax ginseng (PxG) have been reported in both experimental and clinical studies. This study aims to examine the healing effect of PxG on testicular damage induced by MTX. Materials and Methods: Sprague Dawley male rats (8-week-olds) were used in the study. A single dose of MTX dissolved in saline (20 mg/kg) was given to MTX and MTX+PxG groups by intraperitoneal injection. PxG dissolved in saline (100 mg/kg) was given by orogastric gavage once a day for 5 days to the MTX+PxG group. Saline was given to the control and MTX groups orally during the experiments. After decapitation, the testis samples were obtained. Seminiferous tubules and basement membrane were evaluated histopathologically. Seminiferous tubule diameter and germinal epithelium thickness were measured. Furthermore, oxidative stress parameters such as malondialdehyde, glutathione, superoxide dismutase, and glutathione-S-transferase were measured. Results: MTX treatment caused seminiferous tubule degeneration with a decrease in Johnsen’s score, the seminiferous tubule’s diameter, and the germinal epithelium’s thickness. Parallel with the histopathological results increased testicular oxidative stress with an increase in malondialdehyde level and a decrease of endogenous anti-oxidant activity with a decrease in glutathione level and glutathione-S-transferase and superoxide dismutase activities. PxG treatment improved these histological and biochemical parameters in MTX-induced testis cytotoxicity. Conclusion: MTX treatment causes testicular damage via the oxidative processes. PxG treatment ameliorates MTX-induced testicular damage by inhibiting oxidative stress. © 2023 Authors. All rights reserved. en_US
dc.description.sponsorship Marmara Üniversitesi, (TDK-2020-10047) en_US
dc.identifier.doi 10.26650/EurJBiol.2023.1271825
dc.identifier.endpage 37 en_US
dc.identifier.issn 2602-2575
dc.identifier.issue 1 en_US
dc.identifier.scopus 2-s2.0-85166337510
dc.identifier.scopusquality Q4
dc.identifier.startpage 31 en_US
dc.identifier.trdizinid 1188259
dc.identifier.uri https://doi.org/10.26650/EurJBiol.2023.1271825
dc.identifier.uri https://hdl.handle.net/20.500.14627/844
dc.identifier.volume 82 en_US
dc.identifier.wosquality N/A
dc.language.iso en en_US
dc.publisher Istanbul University Press en_US
dc.relation.ispartof European Journal of Biology en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/closedAccess en_US
dc.scopus.citedbyCount 2
dc.subject Methotrexate en_US
dc.subject Oxidative Stress en_US
dc.subject Panax Ginseng en_US
dc.subject Testis en_US
dc.title Morphological and Biochemical Investigation of the Protective Effects of Panax Ginseng on Methotrexate-Induced Testicular Damage en_US
dc.type Article en_US
dspace.entity.type Publication

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
document (5).pdf
Size:
602.83 KB
Format:
Adobe Portable Document Format