Protective Effects Of<i> Rubus</I><i> Tereticaulis</I> Leaves Ethanol Extract on Rats With Ulcerative Colitis and Bio-Guided Isolation of Its Active Compounds: a Combined <i>in Silico</I>, <i>in Vitro</I> and <i>in Vivo</I> Study

dc.authorid dogan, ahmet/0000-0003-0603-5100
dc.authorid Yildirim, Aybeniz/0000-0002-5801-4726
dc.authorid Sen, Ali/0000-0002-2144-5741
dc.authorid Ozbeyli, Dilek/0000-0002-4141-6913
dc.authorid Terali, Kerem/0000-0002-9964-6383
dc.authorid ERTAS, BUSRA/0000-0001-8374-1098
dc.authorid Goger, Fatih/0000-0002-9665-0256
dc.authorscopusid 55250649800
dc.authorscopusid 6504603410
dc.authorscopusid 55867022200
dc.authorscopusid 8926845400
dc.authorscopusid 57215874552
dc.authorscopusid 57196052987
dc.authorscopusid 23017859400
dc.authorwosid yıldırım, aybeniz/GZG-3445-2022
dc.authorwosid Gögel, Fatih/HTR-3454-2023
dc.authorwosid Ozbeyli, Dilek/AAC-2498-2020
dc.authorwosid Ertas, Busra/IQS-8337-2023
dc.authorwosid dogan, ahmet/C-1547-2015
dc.authorwosid Terali, Kerem/Q-3270-2016
dc.contributor.author Şener, Göksel
dc.contributor.author Ozbeyli, Dilek
dc.contributor.author Terali, Kerem
dc.contributor.author Goger, Fatih
dc.contributor.author Yildirim, Aybeniz
dc.contributor.author Ertas, Busra
dc.contributor.author Sener, Goksel
dc.contributor.other Eczacılık Meslek Bilimleri Bölümü
dc.date.accessioned 2025-01-11T13:00:48Z
dc.date.available 2025-01-11T13:00:48Z
dc.date.issued 2023
dc.department Fenerbahçe University en_US
dc.department-temp [Sen, Ali; Yildirim, Aybeniz; Bitis, Leyla] Marmara Univ, Fac Pharm, Dept Pharmacognosy, TR-34854 Istanbul, Turkiye; [Ozbeyli, Dilek] Marmara Univ, Vocat Sch Hlth Serv, Dept Med Serv & Tech, Istanbul, Turkiye; [Terali, Kerem] Cyprus Int Univ, Fac Med, Dept Med Biochem, Nicosia 99258, Cyprus; [Goger, Fatih] Afyonkarahisar Hlth Sci Univ, Fac Pharm, Dept Pharmaceut Bot, Afyon, Turkiye; [Ertas, Busra] Marmara Univ, Fac Pharm, Dept Pharmacol, Istanbul, Turkiye; [Dogan, Ahmet] Marmara Univ, Fac Pharm, Dept Pharmaceut Bot, Istanbul, Turkiye; [Sener, Goksel] Fenerbahce Univ, Fac Pharm, Dept Pharmacol, TR-34758 Istanbul, Turkiye en_US
dc.description dogan, ahmet/0000-0003-0603-5100; Yildirim, Aybeniz/0000-0002-5801-4726; Sen, Ali/0000-0002-2144-5741; Ozbeyli, Dilek/0000-0002-4141-6913; Terali, Kerem/0000-0002-9964-6383; ERTAS, BUSRA/0000-0001-8374-1098; Sener, Goksel/0000-0001-7444-6193; Goger, Fatih/0000-0002-9665-0256 en_US
dc.description.abstract The aim of this study was to evaluate the therapeutic effect of active ethanol extract obtained from the leaves of Rubus tereticaulis (RTME) against colitis, and to purify major compounds from this extract by bioassay-directed isolation. Rats with colitis induced via intra-rectal acetic acid administration (5%, v/v) received RTME or sulfasalazine for three consecutive days. On day four, all rats were decapitated, and the colonic tissue samples were collected for macroscopic score, colon weight, reduced glutathione (GSH), myeloperoxidase (MPO), and malondialdehyde (MDA) analyses. The active compounds and chemical composition of RTME were determined by bio-guided isolation and LC-MS/MS, respectively. Compared to the colitis group, the rats treated with RTME displayed significantly lowered macroscopic scores and colon wet weights (p < 0.001). These effects were confirmed biochemically by a decrease in colonic MPO activity (p < 0.001), MDA levels (p < 0.001), and an increase in GSH levels (p < 0.001). Kaempferol-3-O-beta-D-glucuronide (RT1) and quercetin-3-O-beta-D-glucuronide (RT2) were found to be the major compounds of RTME, as evidenced by in vitro anti-inflammatory and antioxidant activity-guided isolation. Their anti-inflammatory/antioxidant activities were also predicted by docking simulations. Additionally, quinic acid, 5-caffeoylquinic acid, quercetin pentoside, quercetin glucoside, quercetin3-O-beta-D-glucuronide, kaempferol-3-O-beta-D-glucuronide, and kaempferol rutinoside were identified in RTME via using LC-MS/MS. RT2, along with other compounds, may be responsible for the observed protective action of RTME against colitis. This study represents the first report on the beneficial effects of RTME in an experimental model of colitis and highlights the potential future use of RTME as a natural alternative to alleviate colitis. en_US
dc.description.sponsorship Marmara University Scientific Research Projects Coordination Unit [SAG -K- 110117-0001] en_US
dc.description.sponsorship This work has been supported by Marmara University Scientific Research Projects Coordination Unit under grant number SAG -K- 110117-0001. en_US
dc.description.woscitationindex Science Citation Index Expanded
dc.identifier.citation 1
dc.identifier.doi 10.1016/j.cbi.2022.110263
dc.identifier.issn 0009-2797
dc.identifier.issn 1872-7786
dc.identifier.pmid 36375516
dc.identifier.scopus 2-s2.0-85142319014
dc.identifier.scopusquality Q1
dc.identifier.uri https://doi.org/10.1016/j.cbi.2022.110263
dc.identifier.uri https://hdl.handle.net/20.500.14627/72
dc.identifier.volume 369 en_US
dc.identifier.wos WOS:000967045500001
dc.identifier.wosquality Q1
dc.language.iso en en_US
dc.publisher Elsevier Ireland Ltd en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.scopus.citedbyCount 4
dc.subject Antioxidant Activity en_US
dc.subject Anti-Inflammatory Activity en_US
dc.subject Colitis en_US
dc.subject Flavonoids en_US
dc.subject Rubus Tereticaulis en_US
dc.title Protective Effects Of<i> Rubus</I><i> Tereticaulis</I> Leaves Ethanol Extract on Rats With Ulcerative Colitis and Bio-Guided Isolation of Its Active Compounds: a Combined <i>in Silico</I>, <i>in Vitro</I> and <i>in Vivo</I> Study en_US
dc.type Article en_US
dc.wos.citedbyCount 2
dspace.entity.type Publication
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