Effects of <i>crataegus Orientalis</I> Fruit Extract on Lipid Accumulation in Oleic Acid-Induced Hepg2 Cells

dc.authorid Gulmez, Gizem/0000-0002-2036-7892
dc.authorid Sen, Ali/0000-0002-2144-5741
dc.authorscopusid 59001494800
dc.authorscopusid 56031224000
dc.authorscopusid 55250649800
dc.authorscopusid 57487415600
dc.authorscopusid 10038761800
dc.authorwosid şekerler, turgut/AAB-8855-2022
dc.contributor.author Şener, Azize
dc.contributor.author Sekerler, Turgut
dc.contributor.author Sen, Ali
dc.contributor.author Gulmez, Gizem
dc.contributor.author Sener, Azize
dc.contributor.other Temel Eczacılık Bilimleri Bölümü
dc.date.accessioned 2025-01-11T13:02:09Z
dc.date.available 2025-01-11T13:02:09Z
dc.date.issued 2024
dc.department Fenerbahçe University en_US
dc.department-temp [Algul, Kader Fatma; Sekerler, Turgut] Marmara Univ, Fac Pharm, Dept Biochem, Istanbul, Turkiye; [Sen, Ali] Marmara Univ, Fac Pharm, Dept Pharmacognosy, Istanbul, Turkiye; [Gulmez, Gizem] Okan Univ, Fac Med, Dept Med Biochem, TR-34959 Istanbul, Turkiye; [Sener, Azize] Fenerbahce Univ, Fac Pharm, Dept Biochem, Istanbul, Turkiye en_US
dc.description Gulmez, Gizem/0000-0002-2036-7892; Sen, Ali/0000-0002-2144-5741 en_US
dc.description.abstract During the recent years, much great deal of research has been conducted on potential use of certain herbs and nutraceuticals to manage nonalcoholic fatty liver disease (NAFLD), specifically with those strong biological activities and antioxidant ability. In our research, the goal is to investigation the protective efficacy of the ethanol extract of Crataegus orientalis fruits (COE) aganist lipid accumulation in oleic acid (OA)-induced hepatocellular carcinoma cells (HepG2) at the molecular level. Firstly, antioxidant activity and lipoxygenase activity of the C. orientalis were determined in vitro. For induction of lipid accumulation in HepG2, we established an in vitro model using 1 mM OA. The C. orientalis was evaluated for their ability to prevent from lipid accumulation and effects on sterol regulatory element binding protein-1c (SREBP-1c), acetyl CoA carboxylase (ACC1) and fatty acid synthase (FAS) mRNA levels involved in lipid metabolism. The C. orientalis exhibited moderate antioxidant activity against ABTS and DPPH radicals and a significant anti-inflammatory effect against the 5-lipoxygenase enzyme. The results demonstrated that the COE (100 mu g/ml) inhibited lipid accumulation (p < 0.01) and was effective in reducing triglyceride (TG) concentration (p < 0.001) and production of reactive oxygen species (ROS) in HepG2 cells (p < 0.05). C. orientalis suppressed mRNA expression of lipid metabolism enzymes (ACC1, FAS) and transcription factor SREBP-1c (p < 0.001, p < 0.001 and p < 0.01, respectively). Our findings recommend that C. orientalis fruits are potentially protective against lipid accumulation in hepatocytes and may have beneficial effects in the treatment of the NAFLD. [GRAPHICS] en_US
dc.description.sponsorship Marmara University Scientific Research Projects Commission [SAG-C-YLP- 110718-0432] en_US
dc.description.sponsorship This research was supported by Marmara University Scientific Research Projects Commission (Project No: SAG-C-YLP- 110718-0432). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. en_US
dc.description.woscitationindex Emerging Sources Citation Index
dc.identifier.citation 0
dc.identifier.doi 10.1007/s13596-024-00750-5
dc.identifier.endpage 933 en_US
dc.identifier.issn 2662-4052
dc.identifier.issn 2662-4060
dc.identifier.issue 3 en_US
dc.identifier.scopus 2-s2.0-85191294918
dc.identifier.scopusquality Q2
dc.identifier.startpage 923 en_US
dc.identifier.uri https://doi.org/10.1007/s13596-024-00750-5
dc.identifier.uri https://hdl.handle.net/20.500.14627/212
dc.identifier.volume 24 en_US
dc.identifier.wos WOS:001208208400001
dc.language.iso en en_US
dc.publisher Springer en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/closedAccess en_US
dc.scopus.citedbyCount 2
dc.subject Crataegus Orientalis en_US
dc.subject Hepg2 en_US
dc.subject Lipid Accumulation en_US
dc.subject Oleic Acid en_US
dc.subject Lipid Metabolism en_US
dc.title Effects of <i>crataegus Orientalis</I> Fruit Extract on Lipid Accumulation in Oleic Acid-Induced Hepg2 Cells en_US
dc.type Article en_US
dc.wos.citedbyCount 2
dspace.entity.type Publication
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