WoS İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.14627/6
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Article Synthesis, Experimental and Quantum Mechanical Investigation of the Crystal Structures of Two Triazolyl-Indole Compounds, along with the Evaluation of Their Antioxidant Activity(Elsevier, 2026) Pınar, Deniz; Kurt-Şirin, Özlem; Ulusoy-Güzeldemirci, Nuray; Dincel, Efe Doğukan; Karayel, Arzu; Kuran, Ebru DidemIn this study, two 1,2,4-triazolyl-indole compounds, 5-(1H-indol-3-yl)-4-propyl-2,4 dihydro-3H-1,2,4-triazole-3-thione (3a) and 5-(1H-indol-3-yl)-4-butyl-2,4-dihydro-3H-1,2,4-triazole-3-thione (3b), were synthesized and the structures of these compounds were determined by X-ray diffraction method. These two compounds crystallize in the orthorhombic space groups, Pbca. The structures have been determined by direct methods and refined to R, 0.0459 (3a) and 0.0714 (3b). The molecular and crystal structures are stabilized by two intermolecular hydrogen bond for both molecules. The triazole parts of the molecules are almost perpendicular to propyl and butyl groups. NMR studies was also performed to enhance comprehension of the molecular structure of the compounds. The most stable states of the structures, as determined by both DFT analysis and experimental realizations (X-ray, NMR and FT-IR), are the thione form. Although the thione forms are thermodynamically more stable than the thiol forms (Delta H degrees approximate to-15 kcal/mol), they display higher chemical reactivity, as reflected by their smaller HOMO-LUMO energy gaps (Delta E = 4.374 eV for 3a and 4.377 eV for 3b). In addition, the antioxidant activities of both compounds were evaluated using ABTS, DPPH, and FRAP assays. Although both (3a) and (3b) exhibited measurable radical scavenging and reducing power, their antioxidant capacities were found to be lower than that of the reference compound quercetin.Article Citation - WoS: 1Citation - Scopus: 1A Comparative Study of Biochemical, Antimicrobial Effects and Phytochemical Composition Analysis of Glycyrrhiza Glabra L. Varieties Root Extracts(Marmara University, 2025) Sen, Ali; Servı, Hüseyın; Barak, Timur Hakan; Tekin, Fethullah; Şener, Azize; Marzi, Mahdi; Gülmez, GizemPlants are the significant global interest as alternative treatment sources with their biologically activecompounds. This study compares the chemical composition and the antioxidant, antidiabetic, and antimicrobialproperties of ethanol extracts of G. glabra L. two different varieties from different regions. The phytochemicalcompositions was determined using GC-MS. Additionaly, total phenolic (TPC), flavonoid (TFC) and triterpene (TTC)contents were determined. Glycyrrhizic acid contents were analysed by HPLC. G. glabra var. glandulifera (GF1) showedthe highest antioxidant activity. All extracts had strong antidiabetic effects, besides GF1 showing the highest effect. TheMIC values was determined against 8 bacterial and 1 yeast strain and values ranged from 2.500 to 0.500; 2.500 to 0.714;2.500 to 0.714 for G. glabra var. glabra (GB), GF1, G. glabra var. glandulifera (GF2) respectively. Phytochemical studies haveshown that TPC was 100.60±5.06, 127.90±0.30, 69.01±0.30 mg GAE /g extract; TFC was 80.07±0.15, 25.35±0.0, 16.58±0.31mg KE/g and TTC was 217.30±6.05,172.40±2.17, 126.30±4.50 mg OE/g extract for GB, GF1, GF2, respectively. GF1 inparticular has the highest glycyrrhizic acid content. This study will contribute to the creation of new treatment strategiesand potential therapeutic agents in addition to the use of G. glabra L. in traditional treatments. Our study is also apreliminary study for future studies.Article Citation - WoS: 1Citation - Scopus: 1Gold Nanoparticle/Silk Fibroin-Based Nanofiber Enhances Skin Regeneration(Oxford University Press, 2025) Özcan, Ozan; Tufan, Elif; Muhan, Aleyna Tanrıverdi; Çalişkan-Ak, Esin; Şener, Göksel; Tunalı-Akbay, Tuǧba; Ak, EsinObjectives The aim of this study was to determine the wound-healing potential of gold nanoparticles and silk fibroin-based nanofiber produced by green chemistry. Methods The electrospinning method was used to prepare the nanofiber. Twenty rats were exposed to a 7-day treatment period and another 20 rats were exposed to a 21-day treatment period. Rat groups were control, silver, silk fibroin, and silk + gold nanoparticle groups for each period. The effect of the gold nanoparticle/silk fibroin-based nanofiber was examined in skin samples by using biochemical and histological analysis. In biochemical analysis, skin oxidant and antioxidant parameters were determined. Key findings Parameters indicating skin damage returned to their previous levels 7 and 21 days after the wound formation using gold nanoparticle/silk fibroin-based nanofiber. Gold nanoparticle/silk fibroin-based nanofiber initiated hair follicle formation at the wound site and accelerated the re-epithelialization process. Conclusions It was found that the nanofiber prepared by adding gold nanoparticles to silk fibroin had better wound-healing properties than silk fibroin nanofibers without gold nanoparticles. © 2025 Elsevier B.V., All rights reserved.Article Panax Ginseng Extract Ameliorates Methotrexate-Induced Multi-Organ Damage Via the Regulation of Oxidative Stress(Marmara Univ, 2023) Macit, Caglar; Ede-Pazarbasi, Seren; Yilmaz-karaoglu, Suemeyye; Tunali-Akbay, Tugba; Karakaya-Cimen, Fatma Bedia; Ercan, Feriha; Sener, Goksel; Akbay, Tugba Tunalı-Oxidative damage plays an important role in organ toxicities caused by methotrexate (MTX). This study aimed to determine the antioxidant effects of Panax ginseng (PxG) extract against MTX-induced liver, lung, ileum and kidney damage. Twenty-four Sprague Dawley male rats (weight 250-300 g) were used in the study. The animals were randomly separated into three groups: a) Control, b) MTX-treated (MTX) and c) MTX+PxG-treated (MTX+PxG) groups. MTX was administered intraperitoneally at 20 mg/kg, as a single dose to MTX and MTX+PxG groups. PxG was administered orally at 100 mg/kg to the MTX+PxG group for five days. Saline was given to the control and MTX groups for 5 days. At the end of the experiment, liver, lung, ileum, and kidney samples were obtained. Malondialdehyde (MDA) and glutathione (GSH) levels, superoxide dismutase (SOD), glutathione-S-transferase (GST) and tissue factor (TF) activities were determined in all tissues. In addition, histological examinations were done through light microscopy. GraphPad Prism 5v. was used for statistics, and p<0.05 were considered significant. Administration of MTX caused severe injury in tissues. Findings showed that MDA level, SOD, and GST activities were significantly normalized in the MTX+PxG group compared to the control group. A significant reduction in GSH level observed in the MTX group was reversed with PxG administration In addition, TF activity and total protein levels were found to be impaired in the MTX group, but TF activity was significantly improved in liver and lung tissues and total protein level was significantly reversed in lung and ileum in MTX+PxG group. The results of histological examinations showed that MTX-induced damage was ameliorated with the PxG administration. In conclusion, this study shows that Panax ginseng, thanks to its antioxidant properties, reversed MTX-induced tissue damage and therefore may be beneficial against side effects in patients undergoing chemotherapy.Article Citation - WoS: 3Citation - Scopus: 5<i>cotinus Coggygria</I> Scop. Attenuates Acetic Acid-Induced Colitis in Rats by Regulation of Inflammatory Mediators(Springer, 2023) Sen, Ali; Ertas, Busra; Cevik, Ozge; Yildirim, Aybeniz; Kayali, Damla Gokceoglu; Akakin, Dilek; Sener, GokselIn traditional medicine, many medicinal plants are used in the treatment of various diseases caused by inflammation. The objective of the present study is to elucidate for the first time the effects of Cotinus coggygria (CC) ethanol extract (CCE) on colonic structure and inflammation of acetic acid-induced ulcerative colitis in rats. Colonic damage was assessed using disease activity index score, enzyme-linked immunosorbent assay, and hematoxylin-eosin staining. Also, in vitro antioxidant activity of CCE was investigated by ABTS methods. Total phytochemical content of CCE was measured spectroscopically. Acetic acid caused colonic damage according to disease activity index and macroscopic scoring. CCE significantly reversed these damages. While the levels of proinflammatory cytokines TNF-alpha, IL-1beta, IL-6, and TGF-1beta increased in tissue with UC, IL-10 level decreased. CCE increased inflammatory cytokine levels to values close to the sham group. At the same time, while markers indicating disease severity such as VEGF, COX-2, PGE2, and 8-OHdG indicated the disease in the colitis group, these values returned to normal with CCE. Histological research results support biochemical analysis. CCE exhibited significant antioxidant against ABTS radical. Also, CCE was found to have a high content of total polyphenolic compounds. These findings provide evidence that CCE might be benefit as a promising novel therapy in the treatment of UC in humans due to high polyphenol content and justify the use of CC in folkloric medicine for treatment of inflamed diseases.Article Citation - WoS: 4Citation - Scopus: 6Protective Effects Of<i> Rubus</I><i> Tereticaulis</I> Leaves Ethanol Extract on Rats With Ulcerative Colitis and Bio-Guided Isolation of Its Active Compounds: a Combined <i>in Silico</I>, <i>in Vitro</I> and <i>in Vivo</I> Study(Elsevier Ireland Ltd, 2023) Sen, Ali; Ozbeyli, Dilek; Terali, Kerem; Goger, Fatih; Yildirim, Aybeniz; Ertas, Busra; Sener, GokselThe aim of this study was to evaluate the therapeutic effect of active ethanol extract obtained from the leaves of Rubus tereticaulis (RTME) against colitis, and to purify major compounds from this extract by bioassay-directed isolation. Rats with colitis induced via intra-rectal acetic acid administration (5%, v/v) received RTME or sulfasalazine for three consecutive days. On day four, all rats were decapitated, and the colonic tissue samples were collected for macroscopic score, colon weight, reduced glutathione (GSH), myeloperoxidase (MPO), and malondialdehyde (MDA) analyses. The active compounds and chemical composition of RTME were determined by bio-guided isolation and LC-MS/MS, respectively. Compared to the colitis group, the rats treated with RTME displayed significantly lowered macroscopic scores and colon wet weights (p < 0.001). These effects were confirmed biochemically by a decrease in colonic MPO activity (p < 0.001), MDA levels (p < 0.001), and an increase in GSH levels (p < 0.001). Kaempferol-3-O-beta-D-glucuronide (RT1) and quercetin-3-O-beta-D-glucuronide (RT2) were found to be the major compounds of RTME, as evidenced by in vitro anti-inflammatory and antioxidant activity-guided isolation. Their anti-inflammatory/antioxidant activities were also predicted by docking simulations. Additionally, quinic acid, 5-caffeoylquinic acid, quercetin pentoside, quercetin glucoside, quercetin3-O-beta-D-glucuronide, kaempferol-3-O-beta-D-glucuronide, and kaempferol rutinoside were identified in RTME via using LC-MS/MS. RT2, along with other compounds, may be responsible for the observed protective action of RTME against colitis. This study represents the first report on the beneficial effects of RTME in an experimental model of colitis and highlights the potential future use of RTME as a natural alternative to alleviate colitis.Article Citation - WoS: 3Citation - Scopus: 3Investigation of the Protective and Therapeutic Efficacy of <i>myrtus Communis</I> Extract in Aluminum Chloride and Dgalactose-Induced Alzheimer's Disease in Rats(Marmara Univ, 2022) Yalman, Kiibra; Sen, Ali; Cevik, Ozge; Kadioclu-Yaman, Beril; Ertas, Biiva; Yildiz, Sila; Sener, Goksel; Yaman, Beril Kadıoğlu; Kadioğlu-Yaman, BerilThis study investigated the possible protective and therapeutic effects of Myrtus communis subsp. communis ethanol extract (MC) in aluminum chloride (AlCl3) and D-galactose (D-Gal) induced Alzheimer's disease in rats. MC was orally given to rats as a protective treatment for 90 days and, in other two groups starting from the 60th day MC (100-200 mg/kg) was administered, concomitantly with AlCl3 and D-Gal. Learning and memory functions were evaluated by the behavioral tests. Biological activities of MC treatment were examined in hippocampal tissues by ELISA tests. D-Gal and AlCl3-treated rats showed increased amyloid beta (A ss) and 8-hydroxy-2-deoxyguanosine (8OHdG) levels, acetylcholinesterase activity and decreased neprilysin, Na+-K+ATPase and SOD levels in parallel with a decrease in Novel Object Recognition Test, Morris Water Maze and Passive Avoidance Test scores. On the other hand, MC administration reversed the behavioral impairments and improved learning and memory. Moreover, MC treatment decreased A ss and 8-OHdG levels and acetylcholinesterase activity and increased neprilysin levels, Na+-K+ATPase and SOD levels. Our results suggest that MC has beneficial effects on cognitive and neuronal functions through its anticholinesterase and antioxidant properties.Article Citation - WoS: 10Citation - Scopus: 11The Effect of <i>cotinus Coggygria</I> L. Ethanol Extract in the Treatment of Burn Wounds(Marmara Univ, 2022) Erta, Busra; Okuyan, Betul; Sen, Ali; Ercan, Feriha; Onel, Huseyin; Goger, Fatih; Sener, Goksel; Ertas, BüsraThe overall aim of the present research is to evaluate for the first time the curative effect of Cotinus coggygria leaves on burn injury in an experimental burn model along with its anti-inflammatory and antioxidant activity potential. Also, phenolic compounds of C. coggygria were characterised by LC-MS/MS. Wistar albino rats weighing 200-250 g were exposed to 90 degrees C bath for 10 s to induce burn injury, involving 30% of the total body surface area. In the treatment groups, 5% C. coggygria ethanol extract was applied topically as a cream immediately after the burn. Blood and skin tissue samples were taken after decapitation at the 4th and 48th hours following the burn procedure. Interleukin 1-beta (IL-1 beta) and tumour necrosis factor (TNF-alpha) were determined in serum samples, and hydroxyproline, prostoglandin E2 (PGE2), and myeloperoxidase (MPO) activity and 8-hydroxy-2'-deoxy-guanosine (8-OHdG) levels were determined in skin tissue samples. Increased levels of serum cytokines were decreased with C. coggygria treatment in both periods. MPO activity, prostaglandine (PGE2), and 8-OhdG levels increased, while hydroxyproline levels decreased due to burn damage. On the other hand, these parameters were returned to its normal levels with C. coggygria treatment. In addition, the tissue histology of animals treated with C. coggygria showed a complete epithelialization with increased collagenation. As a result, C. coggygria may be an alternative treatment approach for burns-induced skin damage and wounds.