PubMed İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.14627/8
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Article Modulation of Brain Antioxidant Defense, Inflammation, and SIRT1 Activity by a Sunflower Oil-Based High-Fat Diet: Protective Role of L-Arginine in Rats(Springer, 2026) Şekerler, Turgut; Şener, Azize; Çavuşoğlu, Nuray; Doğan, ÖzgeBackgroundChronic consumption of omega-6-enriched dietary fat may disturb brain redox balance and neuroinflammatory homeostasis. Among the sirtuins, sirtuin 1 (SIRT1) exerts critical neuroprotective functions by suppressing oxidative stress and inflammatory signaling; however, the impact of sunflower oil-based high-fat diets (SO-HFD) on brain SIRT1 activity has not been investigated.ObjectiveThis study aimed to investigate the effects of SO-HFD on oxidative stress parameters, inflammatory markers, and SIRT1 activity in rat brain tissue, and to evaluate the potential modulatory role of L-arginine supplementation.MethodsFour-week-old female Sprague-Dawley rats were allocated into three groups: control, SO-HFD, and SO-HFD + L-arginine. Both SO-HFD groups were fed a diet containing sunflower oil for 16 weeks; from week 10 onward, 1.5% L-arginine was supplemented in the drinking water of the SO-HFD + L-arginine group. Following the 16-week protocol, serum and brain specimens were collected. Serum biochemical parameters and adiponectin were quantified; brain homogenates were assayed for lipid peroxidation (MDA), reduced glutathione (GSH), protein thiols (protein-SH), nitric oxide (NO), tumor necrosis factor-alpha (TNF-alpha) levels, and SIRT1 activity.ResultsAlthough brain MDA levels were not significantly elevated, SO-HFD animals exhibited reduced GSH and protein-SH content together with diminished SIRT1 activity. The SO-HFD increased TNF-alpha and NO levels. L-arginine supplementation decreased MDA and increased GSH, protein-SH, and SIRT1 activity. L-arginine also suppressed TNF-alpha levels in brain tissue compared to the SO-HFD group. NO levels in the SO-HFD + L-arginine group were lower than in the SO-HFD group, though not significantly.ConclusionThese findings suggest that chronic exposure to an omega-6-dominant dietary environment disturbs redox regulation and inflammatory balance in brain tissue, accompanied by reduced SIRT1 activity. L-arginine may attenuate cerebral oxidative stress and neuroinflammation by reinforcing endogenous antioxidant mechanisms, highlighting its potential as a nutritional strategy against SO-HFD-induced brain oxidative stress.Article Citation - WoS: 4Citation - Scopus: 4Collagen Peptides and Saccharomyces Boulardii Cncm I-745 Attenuate Acetic Acid-Induced Colitis in Rats by Modulating Inflammation and Barrier Permeability(Wiley, 2025) Altinok, Oyku; Bas, Murat; Dolanbay, Elif Gelenli; Kolgazi, Meltem; Mert, Tugay; Uslu, Unal; Gelenli Dolanbay, ElifUlcerative colitis (UC) is an inflammatory bowel disease characterized by recurrent episodes of inflammation and tissue damage, with limited treatment options. This study aimed to investigate the effects of collagen peptides and Saccharomyces boulardii on acetic acid (AA)-induced colitis. Thirty-six male Sprague-Dawley rats were randomly divided into the following four groups: normal control (NC), colitis control (CC), collagen peptide (CP; 0.6 g/kg/day), and S. boulardii (SB; 250 mg/day). Colitis was induced by an intrarectal administration of AA in all groups except NC, and treatments were administered daily for 7 days. The therapeutic effects were evaluated by assessing the disease activity index (DAI), colon mass index, macroscopic and microscopic tissue damage, histopathological changes, zonula occludens (ZO)-1 protein expression, and myeloperoxidase (MPO) activity. The results showed that CP and SB treatments substantially alleviated DAI scores (p < 0.05) and reduced the colon mass index. Colon macroscopic and microscopic damages improved compared to the CC group (p < 0.01). Histologically, both treatments reduced inflammatory cell infiltration, crypt damage, and ulceration, with CP showing a slightly more pronounced effect. Immunohistochemical analysis revealed significant restoration of ZO-1 protein expression in the treated groups, indicating improvement in intestinal barrier integrity (p < 0.01). Furthermore, MPO activity was reduced in both CP and SB groups, significantly in the SB group (p < 0.01). These findings are consistent with previous studies that highlight the anti-inflammatory and barrier-enhancing effects of collagen peptides and probiotics in UC models.