The Effect of Vitamin D and Paricalcitol on Protein Disulfide Isomerase

dc.contributor.author Koksal, Muhammed Murat
dc.contributor.author Sekerler, Turgut
dc.contributor.author Sener, Azize
dc.date.accessioned 2025-04-11T19:35:48Z
dc.date.available 2025-04-11T19:35:48Z
dc.date.issued 2024
dc.department Fenerbahçe University en_US
dc.department-temp [Koksal, Muhammed Murat] Marmara Univ, Fac Pharm, Dept Biochem, Istanbul, Turkiye; [Sekerler, Turgut; Sener, Azize] Fenerbahce Univ, Fac Pharm, Dept Biochem, Istanbul, Turkiye en_US
dc.description.abstract Protein disulfide isomerase (PDI), a multifunctional protein plays an important role as oxidoreductase, isomerase and chaperone in the cell. Prior studies have identified PDI is highly expressed in many different cancer types and presented as a new potential target for cancer treatment. Here, we investigated vitamin D and its analogue paricalcitol in silico interaction of the human PDI and inhibition of PDI reductase activity in vitro. We observed a non-covalent mechanism where the main skeleton of the vitamin D3 and paricalcitol structure is located at the hydrophobic site in the b' domain of PDI and forms a hydrogen bond with a residue (His138) in this domain. They also form multiple weak hydrophobic interactions with various chemical groups of the b' subunit. For the first time, we demonstrate that 1,25dihydroxyvitamin D3 (1 alpha,25(OH)2 vitamin D3) and paricalcitol inhibit the PDI reductase activity in vitro and their IC50 values are 20.79 +/- 1.43 nmol/L and 32.82 +/- 3.15 nmol/L respectively. The two compounds can also block the denitrosation activity of PDI. en_US
dc.description.woscitationindex Emerging Sources Citation Index
dc.identifier.endpage 2291 en_US
dc.identifier.issn 2630-6344
dc.identifier.issue 6 en_US
dc.identifier.scopusquality Q4
dc.identifier.startpage 2282 en_US
dc.identifier.volume 28 en_US
dc.identifier.wos WOS:001407427700038
dc.identifier.wosquality N/A
dc.language.iso en en_US
dc.publisher Marmara Univ, Fac Pharmacy en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/closedAccess en_US
dc.subject Protein Disulfide Isomerase en_US
dc.subject Paricalcitol en_US
dc.subject Vitamin D3 en_US
dc.subject Molecular Docking en_US
dc.subject Activity Of Enzymes en_US
dc.title The Effect of Vitamin D and Paricalcitol on Protein Disulfide Isomerase en_US
dc.type Article en_US
dc.wos.citedbyCount 0
dspace.entity.type Publication

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