Yüksektepe, Ecem

Profile Picture
Name Variants
Yuksektepe, Ecem
Job Title
Öğretim Görevlisi
Email Address
ecem.yuksektepe@fbu.edu.tr
ORCID ID
0000-0002-8041-2915
Scopus Author ID
Turkish CoHE Profile ID
Google Scholar ID
WoS Researcher ID
GKQ-5228-2022
Scholarly Output

1

Articles

1

Citation Count

1

Supervised Theses

0

Filters

20241
Reset filters

Settings

Scholarly Output Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    Article
    Citation Count: 1
    Functional Groups Matter: Metabolomics Analysis of escherichia Coli Exposed To Trans-Cinnamic Acid and Its Derivatives Unveils Common and Unique Targets
    (Springer, 2024) Yüksektepe, Ecem; Ozturkel-Kabakas, Hatice; Yuksektepe, Ecem; Catav, Sukru Serter; Guzel, Gulnur; Col, Bekir; Elgin, Emine Sonay; Tıbbi Hizmetler ve Teknikler Bölümü
    Phenolic acids are derivatives of benzoic and cinnamic acids, which possess important biological activities at certain concentrations. Trans-cinnamic acid (t-CA) and its derivatives, such as p-coumaric acid (p-CA) and ferulic acid (FA) have been shown to have antibacterial activity against various Gram-positive and -negative bacteria. However, there is limited information available concerning the antibacterial mode of action of these phenolic acids. In this study, we aimed to ascertain metabolic alterations associated with exposure to t-CA, p-CA, and FA in Escherichia coli BW25113 using a nuclear magnetic resonance (NMR)-based metabolomics approach. The results showed that t-CA, p-CA, and FA treatments led to significant changes (p < 0.05) in the concentration of 42, 55, and 74% of the identified metabolites in E. coli, respectively. Partial least-squares discriminant analysis (PLS-DA) revealed a clear separation between control and phenolic acid groups with regard to metabolic response. Moreover, it was found that FA and p-CA treatment groups were clustered closely together but separated from the t-CA treatment group. Arginine, putrescine, cadaverine, galactose, and sucrose had the greatest impact on group differentiation. Quantitative pathway analysis demonstrated that arginine and proline, pyrimidine, glutathione, and galactose metabolisms, as well as aminoacyl-tRNA and arginine biosyntheses, were markedly affected by all phenolic acids. Finally, the H2O2 content of E. coli cells was significantly increased in response to t-CA and p-CA whereas all phenolic acids caused a dramatic increase in the number of apurinic/apyrimidinic sites. Overall, this study suggests that the metabolic response of E. coli cells to t-CA is relatively different from that to p-CA and FA. However, all phenolic acids had a certain impact on oxidative/antioxidant status, genomic stability, arginine-related pathways, and nucleic acid metabolism.