WoS İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.14627/6
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Article Lactobacillus Rhamnosus GG Alleviates Bisphenol-A Induced Oxidative Stress in Serum(Marmara University, Faculty of Pharmacy, 2025) Şener, Göksel; Tunali-Akbay, Tugba; Dorucu, Dogancan; Ede-Pazarbasi, Seren; Dede, Pınar; Ede-Pazarbas, SerenThe objective of this investigation was to identify changes in the serum oxidant-antioxidant balance of rats exposed to bisphenol A (BPA) and to investigate the impact of Lactobacillus rhamnosus GG (LGG) administration on those changes. Twenty-four rats (Wistar Albino, 250-300 grams, male) were divided into control, BPA, and BPA+LGG groups with an equal number of rats. BPA and LGG were applied to the rats in the relevant groups for six weeks, five days each week. Six weeks later, the blood samples were withdrawn and serum samples were prepared. Total oxidant and antioxidant status (TAS), glutathione, and lipid peroxidation determinations were determined in serum samples, and the oxidative stress index was calculated. BPA exposure decreased serum total antioxidant status and increased serum total oxidative status, oxidative stress index, and lipid peroxidation level compared to the control group. LGG administration improved the increased serum oxidative stress caused by BPA. Administration of LGG to BPA-treated rats reversed oxidative stress-induced changes. In conclusion, administration of Lactobacillus rhamnosus GG to rats for 30 consecutive days prevented oxidative stress in serum caused by bisphenol A.Article The Effect of Vitamin D and Paricalcitol on Protein Disulfide Isomerase(Marmara University, 2025) Koksal, Murat; Şekerler, Turgut; Şener, Azize; Koksal, Muhammed MuratProtein disulfide isomerase (PDI), a multifunctional protein plays an important role as oxidoreductase, isomerase and chaperone in the cell. Prior studies have identified PDI is highly expressed in many different cancer types and presented as a new potential target for cancer treatment. Here, we investigated vitamin D and its analogue paricalcitol in silico interaction of the human PDI and inhibition of PDI reductase activity in vitro. We observed a non-covalent mechanism where the main skeleton of the vitamin D3 ans paricalcitol sturcture is located at the hydrophobic site in the b' domain of PDI and forms a hydrogen bond with a residue (His138) in tihs domain. They also form multiple weak hydrophobic interactions with various chemical groups of the b' subunit. For the first time, we demonstrate that 1,25-dihydroxyvitamin D3 (1a,25(OH)2 vitamin D3) and paricalcitol inhibit the PDI reductase activity in vitro and their IC50 values are 20.79±1.43 nmol/L and 32.83±3.15 nmol/L respectively. The two compounds can also block the denistrosation activity of PDI.Review Citation - WoS: 3Citation - Scopus: 4Oral Pharmabiotic Tablet Formulations(Marmara Univ, 2024) Kandur, Buse; Ugurlu, Timucin; Rayaman, Erkan; Ahbaz, Sevinc S.; Şahbaz, SevinçPharmabiotic is a unique and recent term used to describe formulations containing probiotics. Pharmabiotics are probiotics prepared in a pharmaceutical form used to treat diseases and disorders by making physicochemical changes in human health. Probiotics, prebiotics, and synbiotics are included in this scope, and enzobiotics, which are a rising class of supplements, should be evaluated in this context, as they are a subspecies of synbiotics. Pharmabiotics, unlike nutribiotics, do not necessarily contain live microorganisms. The best examples of these are paraprobiotics and postbiotics. Tablet formulations are suitable dosage forms for pharmabiotics due to their redundant superiority over other solid dosage forms. Tablets are frequently preferred because they can be produced at low cost, are easily transported, and modified, are suitable for large-scale production, and are more stable than other dosage forms. Considering the examples in the literature and the definition of pharmabiotic, several tablet formulations can be mentioned as pharmabiotics. They can be divided into conventional uncoated tablets, chewable tablets, and effervescent tablets. With recent studies, this classification has expanded, and buccal mucoadhesive tablets, ODTs (orally disintegrating tablets) and FDTs (fast disintegrating tablets), layered/multi-layered tablets, and tablets within tablets have also taken their place in the classification. This article focuses on oral tablet formulations that can be classified as pharmabiotics.Article The Effects Of<i> Panax</I><i> Ginseng</I> on Serum Oxidative Stress Following Bisphenol a Exposure(Istanbul Univ, 2024) Fazalyar, Najiullah; Pazarbasi, Seren Ede; Dorucu, Dogancan; Sener, Goksel; Tunali-Akbay, Tugba; Ede-pazarbasi, SerenObjective: Bisphenol A (BPA) is a toxic compound that causes oxidative stress by disrupting antioxidant enzymes and promoting tissue lipid peroxidation. This study aimed to examine the impacts of BPA on serum oxidative stress in rats and to detect the antioxidant feature of Panax ginseng (PxG) in reducing BPA-induced oxidative stress. Materials and Methods: Wistar Albino rats (250-300 g) were divided into control, control + PxG, BPA, and BPA + PxG groups. 50 mg/kg BPA and 100 mg/g PxG were given for six weeks. Serum total antioxidant and oxidant status, lipid peroxidation, and glutathione levels were determined. Results: BPA administration increased total oxidant status and lipid peroxidation, while PxG administration to the BPA group decreased these parameters. PxG also increased total antioxidant status and glutathione levels compared to the BPA group. Conclusion: BPA was seen to cause an increase in oxidative parameters and PxG administration to restore the oxidative stress that was generated after BPA exposure, suggesting that this may help to prevent the adverse effects caused by BPA exposure.Article Citation - WoS: 1Citation - Scopus: 1Molecular Modelling Studies To Suggest Novel Scaffolds Against Sars-Cov Target Enzymes(Marmara University, 2021) Şahin, A.F.; Küçükgüzel, Ş.G.; Akdemir, A.In this study, molecular modelling study of previously synthesized compounds against SARS-CoV-2 target enzyme was performed. A subset of 156 compounds from an in-house database has been subjected to molecular modelling studies against the SARS-CoV-2 ADP-ribose phosphatase (ADRP, NSP3), Papain-like protease (PLpro ), and uridine specific endoribonuclease (NSP15) enzymes. We have identified one compound that is expected to inhibit the SARS-CoV-2 ADRP enzyme and one compound that is expected to inhibit the NSP15 enzyme. © 2021 Marmara University Press ISSN: 2630-6344.Article Citation - WoS: 7Effect of Flurbiprofen Derivative (sgk597) on Cell Proliferation and Apoptosis of Breast Cancer Cell Lines(Istanbul Univ, Fac Pharmacy, 2022) Atlihan, Irem; Sevinc, Sevgi Kocyigit; Orun, Oya; Yilmaz, Ozgur; Kucukguzel, S. Guniz; Tiber, Pinar MegaBackground and Aims: The incidence of breast cancer is increasing day by day, especially in women. The search for new drugs against breast cancer is the focus of attention in research. Breast cancer and prostate cancer have remarkable biological similarities. Therefore, the 4-(4-chlorophenyl)-3-(1-(2-fluoro-[1,1'-biphenyl]-4-yl)ethyl)-5-((4-fluorobenzyl)thio)-4H-1,2,4-triazole (SGK597) compound that is suppressing cell proliferation in prostate cancer, was studied in MCF-7 breast can-cer and MCF-10A mammary epithelial cell lines. Methods: The WST-8 method was used to determine cell viability and cytotoxicity of SGK597 in MCF-7 and MCF10-A cell lines. The JC-1 test was applied to determine changes in mitochondrial membrane potential. The protein expression levels of Bax, Bcl-2, and c-PARP associated with apoptosis were determined using Western blot analysis.Results: After 24 and 48 hours of incubation of SGK597, the IC50 values were 28.74 pM and 17.28 pM for MCF-7; 65.9 pM and 50.5 pM for MCF-10A, respectively. Mitochondrial membrane potential showed a tendency toward depolarization in MCF-7 cells as a result of increasing concentration of SGK597, while the same tendency was not seen for MCF-10A. As a result of western blot experiments, no increase in the Bax/Bcl-2 ratio and c-PARP expression level was observed, indicating no apoptosis.Conclusion: It was observed that the compound SGK597 suppressed MCF-7 cell proliferation. These results indicate that SGK597 may be a candidate compound for use as an anticancer agent. Keywords: Apoptosis, breast cancer, flurbiprofen, thioether, triazoleArticle Citation - WoS: 1The Protective Effects Of<i> Momordica</I><i> Charantia</I> Fruit Extract in Methotrexate Induced Liver Damage in Rats(Galenos Publ House, 2022) Ozbeyli, Dilek; Sen, Ali; Cevik, Ozge; Erdogan, Omer; Kaya, Ozlem Tugce Cilingir; Ede, Seren; Sener, Goksel; Ede-pazarbasi, Seren; Cilingir-kaya, Ozlem TugceBACKGROUND/AIMS: Methotrexate (MTX), a cytotoxic therapeutic agent, is used for the cure of malignancies and rheumatologic disorders. However, the significant side effects of MTX limits its use. In this study, we aim to assess the hepatoprotective properties of Momordica charantia (MC) against MTX-induced liver damaged in rats.MATERIALS AND METHODS: Following one dose of MTX (20 mg/kg), the rats were given either distilled water or MC extract (300 mg/kg, po) for 5 days. After the dissection of the rats, the liver was removed to analyse tumour necrosis factor -a (TNF-a), interleukin-113 (IL-113), transforming growth factor 13 (TGF-13) and 8-hydroxy-2'-deoxy-guanosine (8-OhdG) levels and superoxide dismutase (SOD), catalase (CAT), and caspase-3 activities. The tissues were also examined histopathologically.RESULTS: The hepatic TNF-a, IL-113, TGF-13, 8-OhdG levels, and Caspase-3 activity in the MTX group were found to be significantly increased compared to the control group. However, MC extract was able to significantly decrease TNF-a, TGF-13, 8-OhdG levels, and Caspase-3 activity. Also, both the SOD and CAT activity of the MTX group decreased compared to the control group. Although only the SOD levels elevated significantly with MC treatment, the SOD and CAT activities of the MC treated group were similar to the control group. Supporting these biochemical parameters, MTX-induced histologic alterations in the liver were also ameliorated via MC treatment.CONCLUSION: Our results demonstrated that MC has a protective role against MTX-induced hepatic tissue injury by reducing apoptosis, oxidative damage, and the expression of pro-inflammatory cytokines.