WoS İndeksli Yayınlar Koleksiyonu

Permanent URI for this collectionhttps://hdl.handle.net/20.500.14627/6

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  • Article
    Sub-Cytotoxic Mitochondrial Stress in Cardiomyocytes and Whole-Organism Toxicity in C. Elegans Induced by Molnupiravir
    (SAGE Publications Inc, 2026) Gunaydin-Akyildiz, Aysenur; Keskin, Zehra; Ozhan, Gul; Kuran, Ebru Didem; Gulec, Meltem
    Many antiviral agents are known to induce off-target mitochondrial toxicity due to the prokaryotic origin of mitochondria. Mitochondrial dysfunction is frequently linked to cardiotoxicity. We aimed to elucidate the mitochondrial toxicity profile of molnupiravir via focusing on mitochondrial dynamics, biogenesis, and oxidative stress in cardiac cells. Mitochondrial function was evaluated by luminometric measurement of ATP (adenosine triphosphate) content, and flow cytometric analysis of mitochondrial membrane potential, and mitochondrial mass. The expression levels of genes involved in mitochondrial fusion-fission were assessed by RT-PCR. In addition, molecular docking analysis was performed to evaluate the interaction between molnupiravir and the dynamin related-protein DRP1. Protein carbonylation was determined as an oxidative stress parameter. Toxicity evaluation was further investigated in Caenorhabditis elegans to support the in vitro findings at the organismal level. Molnupiravir exposure led to a significant dose-dependent reduction in intracellular ATP level and mitochondrial mass, accompanied by increased protein carbonylation. Mitochondrial membrane potential remained slightly increased. Alterations in the expression of genes regulating mitochondrial dynamics suggested an imbalance between fusion and fission processes, while mitochondrial biogenesis-related signaling was progressively suppressed. C. elegans exposed to higher concentrations of the drug (20-500 & micro;M) exhibited significant lifespan reduction at all doses. Molecular docking analysis demonstrated a moderate binding affinity of molnupiravir to DRP1, supporting a potential direct interaction with mitochondrial fission machinery. In conclusion, our results demonstrate that molnupiravir induces mitochondrial stress through oxidative damage, impaired biogenesis, and altered dynamics, emphasizing the need for careful evaluation of mitochondrial safety of molnupiravir in cardiac tissue.
  • Article
    Content and Quality Analysis of YouTube Videos on Therapeutic Exercises for Parkinson’s Disease
    (SAGE Publications Inc, 2026) Tosun, Anıl; Reyhanioglu, Duygu Aktar
    Background Parkinson's disease (PD) is the most common movement disorder, and patients increasingly use YouTube to obtain health-related information. Objective This study aimed to assess the content quality and informational reliability of YouTube videos on PD exercises. Methods A total of 150 English-language YouTube videos were screened using the search terms Parkinson exercises, Parkinson physiotherapy exercises, and Parkinson home exercise program. For each video, the source, upload date, number of views, likes, dislikes, and comments were recorded. The Video Power Index (VPI) was assessed using the view ratio (views/day) and like ratio (likes & times; 100 / [likes + dislikes]). The clinical quality, reliability, and educational value of PD-specific exercise videos were assessed using the Global Quality Scale (GQS), modified DISCERN (mDISCERN), and guideline-based criteria derived from the European Physiotherapy Guideline for Parkinson's Disease (PD-GEC).Results A total of 29 videos met the inclusion criteria and were analyzed. Videos explaining how and why exercises were performed demonstrated higher mDiscern and GQS scores, while providing repetition, duration, and intensity information was associated with higher GQS scores but not mDiscern (p = 0.080); no differences were observed for disease specificity, functional linkage, or safety warnings (all p > 0.05). PD-GEC scores were not significantly related to video engagement metrics. Conclusion Higher-quality videos tended to provide clear explanations of exercise rationale and dosage, while guideline-based clinical features, including PD-GEC criteria, were not associated with viewer engagement.