WoS İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.14627/6
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Article Citation - WoS: 2Analysis of Selected Steroid Hormones in Sea of Marmara Sediment Samples by Lc-esi/Ms-ms(Istanbul Univ, Fac Pharmacy, 2023) Aysel, Esra; Yurdun, TurkanBackground and Aims: Sediment is the general name given to the muddy structure located at the bottom of aquatic environments such as the sea. In our study, the amounts of steroid hormones were investigated in the sediment samples taken from the Marmara Sea. According to other studies, it has been determined that the excess of the hormone load in the sediments may be an indicator of human/animal sourced pollution, as well as the negative effects of the hormones mixed in the seas with the ecological cycle on the health of humans and animals.Methods: In our study, 31 selected human/animal, plant, natural and synthetic hormone-steroids were studied using Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry (LC-ESI/MS-MS). Methanol and QuEChERS were used as extraction methods. Sediment samples were taken from a total of 27 points selected for sampling at the Marmara Sea.Results: According to the results we found, the androgens: androsterone (24.50-1718.18 ng g-1), testosterone (86.30-1600.32 ng g-1); the estrogens: mestranol (33.73-228.32 ng g-1), equilin (53.44-1232.53 ng g-1); the progestagens; pregnenolone (37.50374.76 ng g-1), progesterone (39.96-405.60 ng g-1); levonorgestrel (325.25 and 937.93 ng g-1); the fecal sterols: cholestanone (57.57-1726.32 ng g-1), coprostanol + epicoprostanol (51.43-1370.33 ng g-1); and the plant sterol; campesterol (35.30-1859.90 ng g-1) were the compounds detected.Conclusion: Estrogens and progestogens are active components of birth control pills, and cholestanone and coprostanol + epicoprostanol are steroids that are indicative of human/animal pollution. Coprostanol + epicoprostanol and cholestanone, which are indicators of fecal pollution, were detected in all sediment samples. In our study, steroid hormones were detected for the first time in Sea of Marmara sediments and possible environmental risks were evaluated.Article Citation - WoS: 3Citation - Scopus: 3Antioxidants, Enzyme Inhibitory Activities, and Phytochemical Profiles of Seven Medicinal Plants Grown With Organic Farming Techniques(Tubitak Scientific & Technological Research Council Turkey, 2023) Zor, Murat; Ozupek, Burcin; Pekacar, Sultan; Deliorman Orhan, Didem; Orhan, Didem DeliormanIn this study, the potential antihyperlipidemic, antidiabetic, and antiobesity effects of extracts obtained via infusion techniques from Artemisia absinthium L., Achillea millefolium L., Calendula officinalis L., Fumaria officinalis L., Mentha piperita L., Origanum onites L., and Urtica dioica L. grown with organic farming techniques were investigated using enzyme inhibition methods (alpha-glucosidase, pan-creatic cholesterol esterase, pancreatic lipase, and alpha-amylase). It is important that natural compounds or extracts to be used in the treat-ment of metabolic problems have antioxidant effects. The antioxidant effects of these species were tested in terms of ABTS and DPPH radical scavenging activity, ferric reducing power, and metal chelating capacity. Extracts (2 mg/mL) of M. piperita (91.43 +/- 0.90%) and O. onites (70.18 +/- 2.02%) showed potent inhibitory effects on the alpha-glucosidase enzyme. Among all species, only O. onites extract ex-erted an inhibitory effect on the cholesterol esterase enzyme close to that of reference compound simvastatin. While all extracts were effective in the tested antioxidant activity methods, it was determined that the M. piperita and O. onites extracts displayed particularly significantly strong activities in terms of ferric reducing power, ABTS radical scavenging effect, and metal chelating capacity. The total flavonoid and phenolic contents of all extracts were determined. The compositions of the most active extracts were analyzed for pheno-lic acids and flavonoids using RP-HPLC. The results of RP-HPLC analysis showed that the levels of ellagic acid (0.923 +/- 0.000 g/100 g extract) in the M. piperita extract and rosmarinic acid (0.813 +/- 0.003 g/100 g extract) in the O. onites extract were high. As a result, all species grown with organic farming techniques except U. dioica were found to have high antioxidant effects. In light of these findings, it was concluded that M. piperita and O. onites grown with organic farming techniques showed strong inhibitory effects in terms of the tested activities, especially for alpha-glucosidase and pancreatic cholesterol esterase, and that in vivo studies and activity-guided isolation studies should be conducted on these species in the future.Article Citation - WoS: 2Citation - Scopus: 2Preparation and Application of Caffeic Acid Imprinted Polymer(Tubitak Scientific & Technological Research Council Turkey, 2023) Ersoy, Seyda K. A. R. A. M. A. N.; Tutem, Esma; Baskan, Kevser SoeZGEN; Apak, ResatIn the present study, molecularly imprinted polymers were synthesized using caffeic acid (CA) as a template molecule and then used for the extraction of CA and chlorogenic acid (CLA) from complex matrices. Syntheses were carried out in tetrahydrofuran as porogenic solvent using 4-vinyl pyridine, methacrylic acid, acrylamide, and 1-vinyl imidazole as monomers, ethylene glycol dimethacrylate as crosslinker and 2,2'-azobisisobutyronitrile as initiator. In polymerization processes, different ratios of the template:monomer:crosslinker (T:M:CrL) were used to obtain the most suitable polymer. Caffeic acid:4-vinylpiridine:ethylene glycol dimethacrylate's 1:4:16 mole ratio of MIP was determined as the most convenient polymer for CA recognition. In addition, nonimprinted polymers (NIPs) without templates were prepared. Dynamic and static adsorption tests were applied to determine the absorption features of the NIPs and CA-MIPs. Separation and purification studies of CA and CLA were performed with molecular imprinted solid phase extraction (MISPE) application. All steps of MISPE (loading, washing, elution) were optimized by HPLC analysis.