WoS İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.14627/6
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Article In Vitro Efficacy of Sumac (Rhus coriaria) Extracts Against Leishmania Tropicana and Leishmania Mexicana: A Preliminary Study from Turkiye(Istanbul Univ, 2025) Mete, Ergun; Ozel, Yener; Bardakci, Hilal; Durmuskahya, Cenk; Koseler, Aylin; Kurt, OzgurObjective: Cutaneous leishmaniasis (CL) is a common clinical manifestation of leishmaniasis. Here, the in vitro anti-leishmanial efficacy of sumac extracts was tested for the first time on both Leishmania (L.) tropica and L. mexicana isolates using Rhus (R.) coriaria plant, which was collected in western Anatolia. Materials and Methods: The dried and powdered fruits of R. coriaria were macerated in acetone, ethyl alcohol, and ethyl alcohol-water mixture at room temperature for two days. The pooled extracts were evaporated under reduced pressure and lyophilized form for the study. Isolates of L. tropica and L. mexicana in Acibadem University R&D Laboratory were initially thawed and cultivated in NNN medium. Assessments were made using the haemocytometer and MTT methods at 24 and 48 h, compared with meglumine antimoniate as the control group. Results: For L. tropica, the effective concentration ranges of the extracts and the infusion were found to be 578.13-289.06 pg/mL and 289.06-144.53 pg/mL, respectively. For L. mexicana, the ranges were found to be 289.06-144.53 pg/mL and 144.53-72.27 pg/mL, respectively. It was shown that all extracts of R. coriaria were effective against both L. tropica and L. mexicana in higher doses, compared to meglumine antimoniate. Conclusion: An interesting finding was that higher sumac doses were required to eliminate L. tropica of the Old World, compared to L. mexicana of the New World. In addition, the aqueous alcohol extract showed efficacy that lasted for 48 h in half doses compared to others in L. tropica. Further assessments for both the identification of the active compounds within R. coriaria and their efficacy in vivo are planned.Article Protective Effects of Cuscuta Sp. Against Cardiorenal Injury in Bile Duct-Ligated Rats(Istanbul Univ, 2025) Hatipoglu, Bilge Nur; Ozbeyli, Dilek; Sen, Ali; Cevik, Ozge; Ercan, Feriha; Albayrak, Omercan; Sener, GokselObjective: Bile duct ligation (BDL) obstructs bile flow, resulting in bile and toxic substances buildup that causes liver damage. This study investigated the protective effects of Cuscuta sp. methanol extract (CUS) against cardiorenal injury in bile duct-ligated rats. Materials and Methods: Rats were categorised into four groups: Control (C), CUS, BDL, and BDL+CUS. The C and BDL groups received saline, whereas the other groups received oral 250 mg/kg CUS. After 28 days, blood, kidney, and heart tissue samples were collected for biochemical and histological analyses. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), direct bilirubin (DB), and total bilirubin (TB) levels were analysed to determine liver function, while Transforming growth factor-beta (TGF-beta) and hydroxyproline (HYP) levels were evaluated for fibrosis, and 8-hydroxy-2-deoxyguanosine (8-OHdG) levels alongside Na+/K+-ATPase activity were analysed to assess oxidative stress and membrane injury in the heart and renal tissues. Results: AST, ALT, DB, and TB levels were significantly elevated in the BDL group compared with the C group; however, the levels were distinctly lower in the BDL+CUS group than in the BDL group. Additionally, in both tissues, TGF-beta, HYP, and 8-OHdG levels were higher in the BDL group than in the C group, but decreased in the BDL+CUS group, with Na+/K+-ATPase activity being lower in BDL group compared with the C group and significantly increased in BDL+CUS group. Conclusion: CUS has protective effects against oxidative damage and offers antioxidant and anti-inflammatory benefits against cholestasis-induced tissue injury.Article Citation - WoS: 3Citation - Scopus: 2Morphological and Biochemical Investigation of the Protective Effects of Panax Ginseng on Methotrexate-Induced Testicular Damage(Istanbul Univ, 2023) Karakaya-Cimen, Fatma Bedia; Macit, Caglar; Sivas, Guzin Goksun; Akbay, Tugba Tunali; Sener, Goksel; Ercan, Feriha; Cımen, Fatma Bedia KarakayaObjective: Methotrexate (MTX) is a chemotherapeutic agent that causes testicular toxicity used in the cure of various types of cancer. The anti-oxidant and anti-cancer effects of Panax ginseng (PxG) have been reported in both experimental and clinical studies. This study aims to examine the healing effect of PxG on testicular damage induced by MTX. Materials and Methods: Sprague Dawley male rats (8-week-olds) were used in the study. A single dose ofMTXdissolved in saline (20 mg/kg) was given to MTX and MTX+PxG groups by intraperitoneal injection. PxG dissolved in saline (100 mg/kg) was given by orogastric gavage once a day for 5 days to the MTX+PxG group. Saline was given to the control and MTX groups orally during the experiments. After decapitation, the testis sampleswere obtained. Seminiferous tubules and basement membranewere evaluated histopathologically. Seminiferous tubule diameter and germinal epithelium thickness were measured. Furthermore, oxidative stress parameters such as malondialdehyde, glutathione, superoxide dismutase, and glutathione-S-transferase were measured. Results: MTX treatment caused seminiferous tubule degeneration with a decrease in Johnsen's score, the seminiferous tubule's diameter, and the germinal epithelium's thickness. Parallel with the histopathological results increased testicular oxidative stress with an increase in malondialdehyde level and a decrease of endogenous anti-oxidant activity with a decrease in glutathione level and glutathione-S-transferase and superoxide dismutase activities. PxG treatment improved these histological and biochemical parameters in MTX-induced testis cytotoxicity. Conclusion: MTX treatment causes testicular damage via the oxidative processes. PxG treatment ameliorates MTX-induced testicular damage by inhibiting oxidative stress.Article The Effects Of<i> Panax</I><i> Ginseng</I> on Serum Oxidative Stress Following Bisphenol a Exposure(Istanbul Univ, 2024) Fazalyar, Najiullah; Pazarbasi, Seren Ede; Dorucu, Dogancan; Sener, Goksel; Tunali-Akbay, Tugba; Ede-pazarbasi, SerenObjective: Bisphenol A (BPA) is a toxic compound that causes oxidative stress by disrupting antioxidant enzymes and promoting tissue lipid peroxidation. This study aimed to examine the impacts of BPA on serum oxidative stress in rats and to detect the antioxidant feature of Panax ginseng (PxG) in reducing BPA-induced oxidative stress. Materials and Methods: Wistar Albino rats (250-300 g) were divided into control, control + PxG, BPA, and BPA + PxG groups. 50 mg/kg BPA and 100 mg/g PxG were given for six weeks. Serum total antioxidant and oxidant status, lipid peroxidation, and glutathione levels were determined. Results: BPA administration increased total oxidant status and lipid peroxidation, while PxG administration to the BPA group decreased these parameters. PxG also increased total antioxidant status and glutathione levels compared to the BPA group. Conclusion: BPA was seen to cause an increase in oxidative parameters and PxG administration to restore the oxidative stress that was generated after BPA exposure, suggesting that this may help to prevent the adverse effects caused by BPA exposure.Article Inequality and the Import Demand in Turkey(Istanbul Univ, 2021) Ozbay Das, Zuhal; Das, Zuhal OzbayThe study aims to see whether the long-run relation between inequality and import demand exists in Turkey. The Autoregressive Distributed Lag (ARDL) cointegration technique is used in this study to estimate the long-run relationships between real imports, income, relative price, real exports, and inequality for the period 1982-2015. The results revealed that the income elasticity of imports is greater than one as the literature suggests. The sign of the coefficient of relative price and its magnitude is also compatible with the literature, while it is not statistically significant. However, the results further reflect that inequality is positively associated with real imports in Turkey contrary to assumptions of the relation between inequality and imports that is found to be negative for lower income countries in some studies. Short-run coefficients reflect that real income and relative prices are associated with real imports, whereas exports and not surprisingly, inequality variables are not in the short-run. The coefficient of income parameter is less in magnitude in the short-run but still greater than one. However, the sign of the coefficient of the relative price turns out to be positive in the short-run.Article Citation - WoS: 1Citation - Scopus: 2The Effect of Whey Proteins on the Brain and Small Intestine Nitric Oxide Levels: Protein Profiles in Methotrexate-Induced Oxidative Stress(Istanbul Univ, 2022) Yilmaz, Sumeyye; Tufan, Elif; Sivas, Guzin Goksun; Gokmen, Begum Gurel; Dursun, Ercan; Ozbeyli, Dilek; Tunali-Akbay, Tugba; Şener, Göksel; Karaoğlu, Sümeyye YılmazObjectives: The aim of this study was to determine the effects of whey proteins on methotrexate (MTX)-induced brain and small intestine damage. Materials and Methods: 30 Sprague Dawley rats (200-300 g) were divided into four groups: Control, control + whey, MTX, and MTX+whey. MTX was administered at 20 mg/kg (single dose) intraperitoneally to the MTX group rats, and 2 mg/kg of whey protein were administered by oral gavage for 10 days to the whey groups. Lipid peroxidation, glutathione, and nitric oxide (NO) levels, as well as glutathione-Stransferase and superoxide dismutase activities were measured in the brain and small intestine. SDS-polyacrylamide gel electrophoresis of the brain and intestine tissues were also carried out. Results: While MTX treatment caused oxidative damage in the brain and small intestine, whey protein administration ameliorated MTXinduced oxidative stress. MTX administration did not change the brain's NO level, while an increase in intestinal NO level was detected. Conclusion: MTX induced oxidative stress in the brain and small intestine changed the protein metabolism in these tissues regardless of reduced food intake. Consecutive 10-day administration of whey proteins has shown its therapeutic effect on MTX-induced brain and small intestine oxidative damage.