WoS İndeksli Yayınlar Koleksiyonu

Permanent URI for this collectionhttps://hdl.handle.net/20.500.14627/6

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  • Article
    Selective Recovery and Preconcentration of Catechins from Wine Industry Residues Using Molecularly Imprinted Polymers: An Eco-Friendly Solid Phase Extraction Approach
    (Springer, 2026) Özen, Seda; Tütem, Esma; Ersoy, Şeyda Karaman
    Molecularly imprinted polymers (MIPs) are engineered materials that provide exceptional selectivity and stability for target molecule recognition. While most previous catechin-imprinted polymer (CAT-MIP) studies have focused on tea matrices, the present study applies CAT-MIP technology to wine industry residues, particularly grape seeds, which represent sustainable and underutilized sources of bioactive compounds. CAT-MIP was synthesized using CAT as the template molecule and polymerization parameters including functional monomer type, porogen composition, and template-to-monomer-to-crosslinker molar ratios were systematically optimized. Among the tested conditions, 4-vinylpyridine (4-VP) and an acetonitrile: dimethyl sulfoxide (ACN: DMSO, 98:2, v/v) solvent system provided the most favorable imprinting performance. Adsorption studies revealed heterogeneous binding behavior consistent with the Freundlich model, while Langmuir parameters were used for comparative evaluation. The optimized CAT-MIP was successfully applied as a molecularly imprinted solid-phase extraction (MISPE) sorbent, achieving selective recovery of 69.20 +/- 2.45% for CAT and 52.50 +/- 1.77% for epicatechin (ECAT) from white grape seed extracts, with comparable performance observed for red grape seed extracts. This MISPE based approach offers the first comprehensive study applying CAT-MIP technology to wine industry waste valorization, comparing red and white grape varieties, and establishing a fully validated HPLC-PDA (high performance liquid chromatography-photodiode array detection) method for CAT quantification. The eco-friendly approach addresses the growing need for sustainable extraction of valuable high-value natural antioxidants. from agro-industrial byproducts.
  • Article
    Metabolic Responses to Benzoic Acid Stress and Glutamine Transport-Dependent Vulnerabilities in Escherichia Coli Revealed by NMR Metabolomics
    (Springer, 2026) Yuksektepe, Ecem; Elgin, Emine Sonay; Onat-Tasdelen, Kadriye Aslihan; Chae, Young Kee; Dogu, Eralp; Catav, Sukru Serter; Ozturkel-Kabakas, Hatice
    Benzoic acid (BA) is a widely used weak organic acid preservative with antimicrobial activity, yet the metabolic basis of its antibacterial action and the determinants of bacterial sensitivity remain incompletely understood. Here we combined growth assays with H-1 NMR metabolomics to characterize BA-induced metabolic responses in Escherichia coli BW25113 and to examine metabolic changes associated with impaired glutamine transport. Wild-type BW25113 and its BA-sensitive isogenic Delta glnP mutant, lacking the membrane-bound glutamine permease of the high-affinity GlnHPQ transport system, were exposed to sublethal BA concentrations. BA slowed growth and significantly altered the levels of 42 metabolites in the wild-type and 38 in Delta glnP, with the mutant showing stronger growth inhibition and reduced BA tolerance. Both strains exhibited metabolic changes consistent with cellular responses to oxidative and acid stress, including alterations in central carbon metabolism, lysine degradation, cysteine and methionine metabolism, pyrimidine metabolism, and one-carbon pool by folate. However, several metabolic responses differed between the two strains. In wild-type cells, BA exposure was associated with changes in glycerolipid metabolism, glycerophospholipid metabolism, nicotinate and nicotinamide metabolism, lysine biosynthesis, glycine, serine and threonine metabolism, and purine metabolism. In contrast, Delta glnP cells showed distinct alterations in D-amino acid metabolism, arginine biosynthesis, and other carbon fixation pathways. In addition, the mutant displayed substantial baseline differences relative to the wild-type, including altered nucleotide and amino acid pools. Together, these results indicate that both BA exposure and deletion of glnP induce broad metabolic adjustments in Escherichia coli. Loss of glnP is associated with distinct metabolic states and altered responses to BA stress, highlighting the importance of glutamine transport in adaptation to weak organic acid stress.
  • Article
    Ibuprofen and Nimesulide Derivatives Selectively Induce Apoptosis in HER2-Positive Breast Cancer via Inhibition of the PLA2-COX-2-NF-κB Pathway
    (Springer, 2026) Bedir, Ipek; Cakirli, Egemen; Kucukguzel, S. Guniz; Yilmaz, Ozgur; Biliz, Yagmur; Telci, Dilek
    Background Chronic inflammation contributes to breast cancer development through the phospholipase A(2) (PLA(2))-cyclooxygenase-2 (COX-2)-nuclear factor kappa B (NF-kappa B) cascade, which regulates prostaglandin synthesis, oxidative stress, and transcription of pro-inflammatory and anti-apoptotic genes. This pathway is particularly active in HER2-positive breast cancer, promoting proliferation, invasion, and resistance to apoptosis. Non-steroidal anti-inflammatory drugs such as ibuprofen and nimesulide target COX enzymes and have shown potential in suppressing inflammation-driven tumorigenesis. In this study, we evaluated the anticancer and anti-inflammatory activity of newly synthesized, structurally modified ibuprofen and nimesulide derivatives designed to modulate PLA(2)-COX-2-NF-kappa B axis. Methods and Results Cytotoxicity was assessed in HER2-positive breast cancer cells (AU565 and SKBR3) and compared with normal dermal fibroblasts (HDF) and breast epithelial cells (MCF-12A), using WST-1 assays. Apoptosis, cell cycle distribution, caspase-3/7 activation, and ROS generation were analyzed by imaging-based assays, flow cytometry, and fluorescence methods. Gene expression of PLA2G2A and PTGS2 was quantified by qRT-PCR, and NF-kappa B translocation was analyzed by immunocytochemistry. Two ibuprofen triazole derivative (D1) and ibuprofen thioether derivative (D7) and one nimesulide derivative (D8) significantly reduced cell viability in a dose-dependent manner without affecting normal cells. These derivatives induced G(0)/G(1) arrest, caspase-3/7 activation, ROS reduction, and increased late apoptosis. Downregulation of PLA2G2A and PTGS2 expression and inhibition of NF-kappa B translocation confirmed disruption of the PLA(2)-COX-2-NF-kappa B cascade. Conclusion These findings demonstrate that structurally optimized ibuprofen and nimesulide derivatives exert dual anti-inflammatory and anticancer effects in HER2-positive breast cancer by suppressing PLA(2)-COX-2-NF-kappa B pathway and promoting apoptotic cell death.
  • Article
    Determination of the Susceptibility of Onychomycosis Agents Isolated in Sakarya to Octenidine Dihydrochloride and Hypochlorous Acid
    (Springer, 2026) Köroğlu, Mehmet; Çiftci, Ihsan Hakkı; Gül, Merve; Yağmur, Ahmet Can; Aydemir, Özlem; Kılbaş, İmdat; Erman, Gülay
    The aim of this study was to investigate the susceptibility of onychomycosis agents to octenidine dihydrochloride (OCT-D) and hypochlorous acid (HOCl) antiseptics using agar dilution, tube dilution, and flow cytometry methods. In this study, onychomycosis agents including dermatophytes (Trichophyton spp., Microsporum spp., Candida spp.) and secondary agents such as Aspergillus niger and Fusarium solani were cultured on Sabouraud dextrose agar (SDA). Species identification was confirmed using MALDI-TOF MS. The minimum inhibitory concentration (MIC) values of OCT-D, HOCl, and terbinafine for dermatophytes grown on culture plates were determined using the agar dilution method and the broth microdilution (tube dilution) method in accordance with the European Committee on Antimicrobial Susceptibility Testing (EUCAST) guidelines. Following MIC determination, the minimum fungicidal concentration (MFC) was assessed. Based on the MFC results, flow cytometry analysis was performed using SYTO 9 and propidium iodide (PI) dyes to investigate the fungicidal effects of OCT-D, HOCl, and terbinafine.As a result of antifungal susceptibility testing of onychomycosis agents, approximate MIC values obtained by the agar dilution method were 250 mg/L for OCT-D, 128 mg/L for HOCl, and 0.064 mg/L for terbinafine. Using the tube dilution method, approximate MIC values were 62.5 mg/L, 64 mg/L, and 0.032 mg/L for OCT-D, HOCl, and terbinafine, respectively. Flow cytometry-based viability analyses showed that the results obtained using the tube dilution method were more consistent. Since OCT-D and HOCl demonstrated MIC values far below their commercially available concentrations and exhibited high fungicidal activity, further studies suggest that these agents may be strong alternatives for topical treatment.
  • Article
    The Relationship Between Spiritual Well-Being, Resilience, and Adherence Among Patients Receiving Hemodialysis Treatment in Türkiye
    (Springer, 2026) Bulbul, Elif; Sukut, Ozge; Dikec, Gul
    This study examined the relationship between spiritual well-being, resilience, and adherence of hemodialysis patients and the factors affecting them. The data were collected from 182 hemodialysis patients receiving treatment in a dialysis center who met the inclusion criteria by purposive sampling method in Istanbul. The data were collected with the patient description questionnaire, which measures patient sociodemographic characteristics and characteristics related to the medical diagnosis, the Spiritual Well-Being Scale, the Brief Resilience Scale, and the End-Stage Renal Failure-Adherence Questionnaire. Gender, educational status, employment status, and mean age of patients were found to be correlated with psychological resilience. Marital status, employment status, cohabitants, and mean age of patients were found to be correlated with spiritual well-being. Gender, number of weekly dialysis sessions, and dialysis competencies were found to be correlated with hemodialysis patients' adherence to their treatment. Hemodialysis patients' adherence was positively correlated with both the faith subscale of spiritual well-being and psychological resilience. According to regression analysis, gender and resilience explained 12.8% of the total variance of adherence. This study determined that resilience is an essential factor in increasing the adherence of hemodialysis patients.
  • Article
    Advances and Strategies in Biosensor-Based Diagnostics for Parasitic Infections: A Comprehensive Scoping Review
    (Springer, 2026) Aminizadeh, Selva; Alizadeh, Gita; Alizadeh, Zahra; Khalilzadeh, Balal; Abidin, Zurina Zainal; Marzi, Mahdi; Rafiei-Sefiddashti, Raheleh
    Parasitic diseases are among the most widespread infections worldwide, causing millions of deaths and illnesses each year. So rapid and accurate diagnosis is essential, requiring highly sensitive and specific tests. Biosensors can provide significant advantages over traditional diagnostic methods because of their specificity, sensitivity, speed, simplicity, ease of use, repeatability, and capacity for early-stage disease detection. Recent advances in modern diagnostic tools for detecting parasitic infections use nanomaterials such as gold nanoparticles, carbon nanofibers, and carbon nanotubes. These developments have significantly lowered detection limits to the picogram and femtogram levels. This review will cover recent advancements in biosensor-based diagnostic techniques in parasitology.
  • Article
    Citation - WoS: 2
    Citation - Scopus: 1
    Comprehensive Bibliometric Analysis of Characteristics, Patterns, and Causes of Retractions in Pediatric Literature
    (Springer, 2025) Abo-Elnour, Dina Essam; Helal, Mohamed Mohsen; Albalasy, Abdulrahman Ahmed; Abdul-Hafez, Hamza A.; Abdelkader, Ahmed; El-Sherbini, Eman Raafat; Amer, Samar A.
    This study aimed to systematically identify the key characteristics of retracted articles in pediatric literature and explore the patterns and reasons of pediatric retraction from 1995 to 2024. We searched PubMed and Retraction Watch databases to identify all retracted publications in the field of pediatrics. After the screening process, data were extracted into Excel. Statistical analysis was conducted using Jamovi and Excel. A correlation matrix was used for the important retraction-related factors. After screening, 590 unique retracted pediatric articles were included, with most of them, 572 (96.9%), having retraction notices available; 516 (87.5%) published as open access; 433 (73.4%) from Asia-mostly China; 348 (59%) retracted by the publisher; 301 (51.0%) published by Hindawi; 275 (46.6%) observational studies; and 221 (37.5%) retracted due to misconduct. Articles with four authors showed the highest retraction rate, and the retraction rate generally decreased as the number of authors increased. Most retractions occurred in 2023. The most common pediatric age group included in the retracted papers was children. The median H-index of authors of retracted papers was 8 for first authors and 10 for senior authors. The median time from submission to acceptance of retracted papers was 50 days and that from publication to retraction was 15 months. Additionally, time to retraction was positively correlated with the journal's impact factor (r = 0.106, p = 0.015) and the citation count (r = 0.213, p < 0.001) but showed no significant correlation with time to acceptance (r = - 0.019, p = 0.675). Conclusion: The increasing number of retracted pediatric papers reflects a growing concern with a complex pattern and various determinants. Researchers and publishers should adopt strong regulations and guidelines to improve the integrity of scientific research, especially pediatric research.
  • Article
    Google Trend Index as an Investor Sentiment Proxy in Cryptomarket: Nonlinear Relationships With Cryptomarket and Predicting Bitcoin Returns With Machine Learning Approach
    (Springer, 2025) Koy, Ayben; Demir, Semra; Colak, Andac Batur
    This study investigates the utility of Google trend indices as proxies of investor sentiment, examining their relationships with cryptocurrency market prices and their potential for return prediction. Employing several nonlinear econometric models including the momentum threshold autoregressive AR (MTAR), Kapetanios, Shin, and Snell, and exponential smooth transition autoregressive vector error correction model, the research the relationships between Google trend indices and BTC prices. Additionally, the study evaluates the performance of three developed artificial neural network models in predicting bitcoin returns based on investor sentiment derived from Google trend indices. The findings highlight that the MTAR model effectively captures significant relationships between the variables studied. However, predicting bitcoin returns remains challenging due to their typically small values, which represent the changes between observation points.
  • Article
    Recognition of Clove (Eugenia Caryophyllata) Oil Adulteration by Monitoring Phenolic and Antioxidative Fingerprint Markers Utilizing Online HPLC Post Column and Spectrophotometric Assays With Chemometric Analysis
    (Springer, 2025) Ersoy, Seyda Karaman; Kaya, Elif Nilay; Celik, Saliha Esin; Senol, Onur; Apak, Resat; Karaman Ersoy, Şeyda
    This research aims to develop a reliable and versatile methodology for authenticating clove (Eugenia caryophyllata) oil (CLO). Key variables included total antioxidant capacity (TAC), total phenolic content (TPC), quantitative analyses and/or individual antioxidant capacity of fingerprint markers including eugenol (EUG) and tocopherol isomers (alpha-T, gamma-T, and delta-T) using reversed-phase liquid-chromatography (RP-HPLC) with post-column detection. A total of 28 commercial CLO samples (certified as 100% pure and other commercial samples), potential adulterants like vegetable oils (sunflower oil (SFO) and corn oil (CO)), and 14 synthetically adulterated CLO samples blended with varying proportions (ranging from 5 to 50%) of SFO and CO were analyzed. The EUG content and antioxidant capabilities of each marker were ascertained by online RP-HPLC analysis with post column detection utilizing CUPRAC (cupric reducing antioxidant capacity). EUG content in commercial CLO ranged from 1.46 to 98.06 mg g(-1), while TAC ranged between 18.54 and 748.46 mu mol g(-1) Trolox equivalents (TE), as determined by the online HPLC-CUPRAC method. Depending on the type and ratio of adulterating oils, a considerable decrease in the TAC values of virgin CLO was recorded. Classification of the commercial and synthetically adulterated CLOs (total of 42 samples) was performed using partial least square discrimination analysis (PLS-DA) and hierarchical cluster analysis (HCA). Adulteration levels above > 5% were successfully detected with 95% confidence. Thus, the proposed chemometric strategy combining selected chemical markers and TAC data demonstrated high potential for authenticating CLO. This technique provides a more focused investigation alternative for determining the authenticity and quality of commercial CLO.
  • Article
    Unraveling the Persistent Renal Impact of Intrauterine Growth Restriction and Catch-Up Growth: Integrating Morphological Insights with Metabolomic Profiling
    (Springer, 2025) Esrefoglu, Mukaddes; Koktasoglu, Fatmanur; Bayindir, Nihan; Cimen, Fatma Bedia Karakaya; Kirmizikan, Seda; Hekimoglu, Emine Rumeysa; Selek, Sahabettin
    The study aimed to investigate the long-term effects of IUGR and consequent catch-up growth on metabolic health by using a comprehensive approach that included histopathological, immunohistochemical, biochemical, and metabolomics analyses. Sprague-Dawley pregnant rats either undergo bilateral uterine artery ligation or a sham surgery on the 19th day of gestation. The offspring reached catch-up growth, kidney samples were collected at postnatal weeks 2, 4, and 8 for analysis. IUGR rats exhibited a spectrum of changes including reduced glomeruli number, proliferating cell number, altered oxidative stress markers, various enzymes involved in Krebs cycle, mitochondrial dynamics, and energy metabolism. Examination of the 8-week-old cohort identified a broader spectrum of metabolic alterations, notably in the biosynthesis of phenylalanine, tyrosine, and tryptophan, phenylalanine, tyrosine, glyoxylate, dicarboxylate, pyruvate, alanine, aspartate, and glutamate metabolism, glycolysis/gluconeogenesis and citrate (TCA) cycle. Our metabolomics analysis provides insights into the potential disease susceptibility of individuals born with IUGR, including obesity, diabetes, hypertriglyceridemia, cardiovascular diseases, and mental retardation. These findings underscore the intricate interplay between intrauterine conditions and long-term metabolic health outcomes, highlighting the need for further investigation into preventive and therapeutic strategies to mitigate the risk of metabolic diseases in individuals with a history of IUGR.