WoS İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.14627/6
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Article Comprehensive Proteomic and Metabolomic Analysis of Novel Substituted Fluoroquinolone Derivatives in Escherichia Coli Isolates(John Wiley and Sons Ltd, 2026) Nigiz Ş.; Kulabaş N.; Türe A.; Kablan S.; Koçak E.; Özkul C.; Küçükgüzel İ.; Koçak, Engin; Nigiz, Şeyma; Kablan, Sevilay Erdoğan; Özkul, Ceren; Kulabaş, Necla; Küçükgüzel, İlkay; Türe, AslıAntimicrobial resistance is one of the most important global problems, and new antibiotic requirements have been emerging as a key point in this issue. In the present work, we focused on the efficiency of two novel promising fluoroquinolone derivatives on resistant Escherichia coli isolates at the molecular level. Their mode of action and adaptation process were evaluated by using proteomics and metabolomics analysis. Proteomics analysis showed that two compounds have an effect mainly on the ribosomal process and energy metabolism. Moreover, we observed compounds that affect various important antimicrobial targets, such as ribosomal subunits, phosphotransacetylase, and chaperone proteins. In metabolomics analysis, we found that compounds altered bacterial metabolism directly. Pathway analysis showed that cofactor biosynthesis and energy metabolism were affected mainly by undertreated groups. Our experiments demonstrated that novel fluoroquinolone derivatives have promising results at the molecular level and results will contribute to further studies. © 2026 John Wiley & Sons Ltd.Article Fully Synthetic, Nature-Inspired Exosome-Mimetics for Melanoma Therapy(Elsevier B.V., 2026) Arda Ozturk N.Z.; Majchrzak O.B.; Ulivi G.; Kirmizibayrak P.B.; Borchard G.; Patrulea V.; Ozer O.; Ozturk, Nahide Zeren Arda; Borchard, Gerrit; Ozer, Ozgen; Patrulea, Viorica; Majchrzak, Oliwia Barbara; Arda Ozturk, Nahide Zeren; Kirmizibayrak, Petek Ballar; Ulivi, GianlucaFully synthetic exosome-mimetics (FSEMs) represent a nature-inspired drug delivery system designed to replicate the key physicochemical and biological properties of natural exosomes, while offering the potential to address limitations in scalability and reproducibility associated with natural exosomes. In this study, we prepared FSEMs at the laboratory scale. We loaded them with (–)-epigallocatechin-3-gallate (EGCG) and microRNA-23a (miR-23a), aiming to co-deliver therapeutic small molecules and nucleic acids for the treatment of melanoma. FSEMs were fabricated using three methods: thin-film hydration, ethanol injection, and microfluidics. They were surface-functionalized with either CD9, a tetraspanin involved in membrane fusion, or TSP-1, an adhesion protein promoting cellular interactions. Through physicochemical characterization via dynamic light scattering, we found that FSEMs were ∼ 100 nm in size, of low polydispersity (∼0.2) and showed a negative zeta potential (∼–55 mV). Both EGCG and miR-23a were efficiently encapsulated. SDS-PAGE analysis confirmed successful protein incorporation and correct positioning. In vitro release studies showed minimal premature leakage, supporting their suitability for cellular uptake-mediated delivery. When tested on melanoma cells (MDA-MB-435) and progenitor human dermal fibroblasts (FE002-SK2), FSEMs selectively killed melanoma cells while sparing fibroblasts. Importantly, EGCG within FSEMs was more effective than the free compound. Compared to conventional DOTAP-based liposomes, FSEMs were more selective and induced less off-target cytotoxicity. This study presents a proof-of-concept for fully synthetic, protein-functionalized FSEMs as dual carriers for both chemical and gene-based agents, offering a safer and potentially more effective alternative to traditional cationic liposomes. These results lay the groundwork for future in vivo validation and translational cancer research. © 2026 The Author(s)Article Components of Design Thinking in Spatial Design Education and a Model Proposal(Konya Technical Univ, Fac Architecture & Design, 2025) Coruk, Ipek YildirimDesign thinking, defined in its simplest form as a creative problem-solving process, is interpreted in various ways in the literature, with differing models and components. This study, motivated by the need to identify gaps in existing approaches and to clarify the concept of design thinking, aims to define its key components within the context of spatial design education. To achieve this objective, the study employed qualitative research methods. To collect data, the study utilized both a literature review and content analysis techniques. By categorizing the collected data, it was concluded that design thinking consists of three fundamental components: cognitive-rational, emotional-intuitive, and practical. These components were elaborated upon with subcategories based on literature data, and a comprehensive model proposal for use in space design education was developed. The proposal put forward in this study is significant in clarifying the concept of design thinking and its constituent components. On the other hand, the potential of the proposed model to offer guiding alternatives for the problem-solving process in design studios at various levels and to make design thinking more explicit can be attributed to the pedagogical contributions of this study. From a practical perspective, the study is considered to have the potential to directly inform practice by proposing concrete and applicable steps that can be implemented within the design process. For future research based on this study, it is recommended that the potential contributions of the proposed model be explored through its application in actual design processes. Furthermore, expandingthe theoretical scope by questioning different approaches to the components of design thinking is also suggested.Article Sexual Problems of Women with Kidney Transplant: A Qualitative Study(Galenos Publ House, 2026) Akinci, Naile; Varisoglu, Yeliz Yildirim; Dogan, BayramObjective: This qualitative study aimed to explore the experiences, perspectives, and challenges faced by women who underwent kidney transplantation, particularly regarding the impact of transplantation on their own and their partner's sexual lives. Methods: The study was conducted with 15 women who had received kidney transplants at a private hospital in & Idot;stanbul. Data were gathered using a two-part semi-structured interview form developed by the researcher based on a review of the relevant literature. The data obtained from the interviews were analyzed using content analysis. Data analysis was carried out concurrently with data collection. This study adhered to the consolidated criteria for reporting qualitative research. Results: Based on a thematic analysis of the interviews, four main themes emerged: concerns about reproductive health, including subthemes of fear of infertility and anxiety about pregnancy; disease-associated sexual reluctance, including subthemes of reduced sexual interest, fatigue, weakness, sleep disturbances, and depression; perception of femininity and body image, including subthemes of feelings of incompleteness and inadequacy; concerns about the spouse/partner, including subthemes of fears about being unable to meet the sexual needs of the spouse/partner and feelings of guilt related to their partner's sexual dissatisfaction. Conclusion: In conclusion, sexual dysfunction continues to persist among women even after kidney transplantation due to various physical and psychological factors. To support patients in maintaining a healthy sexual life as part of their overall well-being, sexual health should be routinely assessed by a multidisciplinary team, including transplant surgeons, surgical and obstetric/ gynecology nurses, and psychologists.Article Social Media and Financial Decisions: The Influence of Socio-Demographics and Financial Literacy(John Wiley and Sons Inc, 2026) Altinbas, H.This study investigates the predictors of individuals' reliance on social media for financial decision-making within the context of Türkiye's high-inflation environment and the associated surge in retail investor participation. Data were collected via an online survey utilizing the OECD's financial literacy toolkit. The results indicate that gender, family structure, high-risk asset preferences, and financial literacy predict social media usage for financial information. Specifically, males, individuals who invest in stocks or cryptocurrencies, and those with higher financial literacy demonstrate a greater propensity to access financial information on social media; conversely, households with children exhibit lower reliance on social media information. © 2026 American Association of Family and Consumer Sciences.Article Investigation of the Susceptibility Rates to Ceftazidime-Avibactam and Colistin, Clonal Relationships, and Clinical Data of Patients with Carbapenem-Resistant Klebsiella Pneumoniae Isolates Detected in the ICUs of a Hospital in İstanbul(K Faisal Special Hospital Research Centre, 2026) Akkaya, Yuksel; Aydin, Irfan; Harmankaya, Sebile; Karakul, Mehmet; Aydin, Mehtap; Erdin, Begum Nalca; Kilic, Ibrahim Halil; Karakuş, MehmetBACKGROUND: The increase in carbapenem-resistant K. pneumoniae (CR-Kp) in intensive care units (ICUs) causes treatment difficulties and increases risk in mortality. OBJECTIVES: The aim of this study was to investigate the susceptibility rates of CR-Kp isolates obtained from ICUs to ceftazidime-avibactam (CAZ-AVI) and colistin, carbapenem resistance genes, clonal relationships and clinical characteristics of the patients. DESIGN: Retrospective cohort SETTING: Single-center, University of Health Sciences, & Uuml;mraniye Training and Research Hospital MATERIALS AND METHODS: This study was conducted between June 2023 and December 2024. Isolates were identified using VITEK MS v.3.2, and antibiotic susceptibility testing was performed using the VITEK 2 Compact system. CAZ-AVI susceptibility was determined using disk diffusion, and colistin susceptibility was determined using broth microdilution to determine minimum inhibitory concentration (MIC) values. Carbapenem resistance genes were determined using multiplex real-time polymerase chain reaction (RT-PCR) and clonal relationship arbitrarily primed-polymerase chain reaction (AP-PCR). MAIN OUTCOME MEASURES: Resistance genes of CR-Kp isolates, clonal relationships, CAZ-AVI and colistin resistance, and clinical characteristics of patients SAMPLE SIZE: Ninety-seven isolates from 76 patients RESULTS: Among patients with CR-Kp isolates, central venous catheter use was detected in 59 cases (78%), ventilator-associated pneumonia in 44 cases (58%), and bacteremia in 39 cases (51%), respectively. It was determined that 53 of the patients (70%) died. Using the AP-PCR method, 60 different genotypes were identified among 97 isolates, and clustering was determined in 42 of the isolates (46%). It was determined that 36 (37%) of the isolates were resistant to colistin and 42 (45%) were resistant to CAZ-AVI. NDM+OXA-48, OXA-48, KPC, KPC+NDM, and NDM genes were detected in 40 (43%), 32 (35%), 10 (11%), 2 (2%), and 3 (3%) isolates, respectively. It was determined that 30 (75%) of the isolates with NDM+OXA-48 and only 4 (12%) of the isolates with OXA-48 were resistant to CAZ-AVI. CONCLUSION: In addition to OXA-48, an increase in the frequency of CR-Kp isolates containing the NDM, NDM+OXA-48, KPC+NDM, and OXA-48+KPC genes were also detected. It was also determined that resistance to colistin and CAZ-AVI is increasing. The AP-PCR method can also be used to investigate infections. LIMITATIONS: Single center,Pulsed Field Gel Electrophoresis (PFGE) could not be performed together with AP-PCRArticle Does Localization Change Management in Complicated Right and Left-Sided Diverticulitis(Baishideng Publishing Group Inc, 2025) Acar, Turan; Sur, Yunus; Acar, Nihan; Tekindal, Mustafa Agah; Dilek, Osman NuriBACKGROUND Distribution of the colonic diverticula differs in different populations, and right-sided colon diverticulitis (RCD) and left-sided colon diverticulitis (LCD) manifest distinct clinical features. Complicated diverticulitis (CD) mostly requires hospitalization and can be treated within a spectrum from observation to surgery. Treatment choice is formed depending on the patient's general condition, the presence of diffuse peritonitis, the localization of diverticulitis, Hinchey stage, and responsiveness to the prior treatment. Clinical disparities regarding right and LCD also led to the differences in the incidences of both emergency surgery and future elective surgery. AIM To evaluate the clinical features of CD, display the differences according to colonic localizations, and present treatment approaches. METHODS This was a retrospective study from a single centre analysing data from a prospective database. The 253 patients with history of hospitalization for CD were included and divided into two groups: RCD and LCD. To compare the differences between the two groups, the Student's t-test was used when the parametric test prerequisites were fulfilled, and the Mann-Whitney U test was used when such requirements were not fulfilled. RESULTS The 208 (82.2%) patients were found to have LCD, and 45 (17.8%) had RCD. The majority of the patients had Hinchey 1A diverticulitis (49.8%). Male gender was significantly more common in patients who underwent surgery for LCD. While persistent abdominal pain was the main prior finding in the conservative treatment of both localizations, surgery was most performed due to abscess in RCD and perforation in LCD. The presence of an accompanying malignancy during colonoscopy was significantly more common in LCD cases who underwent surgery. Hartmann's procedure was the most performed technique in emergency settings (56.3%), while laparoscopic colectomy with anastomosis was in elective settings (53.9%). In addition, surgery was found to prolong the mean length of hospital stay in LCD patients. CONCLUSION Although diverticulitis is a benign condition, the need for an individualized and evidence-based approach makes management challenging. Localization of the disease has an important role in determining the appropriate treatment.Article Pre-Service Physical Education Teachers' Teacher Identity Scale (PPET-TI): Adaptation Study to Turkish Culture(Hacettepe University, 2026) Sonmez, Huseyin Ozan; Asci, Fevziye Hulya; Mirzeoglu, Ayse Dilsad; Asci, HülyaIn Turkey, the lack of measures for assessing the teacher identity of pre-service physical education teachers remains a significant challenge. Developing quantitative instruments grounded in a robust theoretical framework is crucial, as such tools would not only advance research in this field but also contribute to improving the quality of physical education teacher education. This study aims to test the reliability and validity of the Pre-service Physical Education Teachers' Teacher Identity Scale (PPET-TI) to Turkish pre-service physical education teachers. The PPET-TI is a 7-point Likert-type scale comprising 17 items and three subscales: self-definitions, teaching goals, and professional responsibilities. Two hundred fifty pre-service physical education teachers (96 females and 154 males; (x) over bar (age) = 21.25 +/- 2.36) voluntarily participated in the study. Confirmatory Factor Analysis (CFA) and Exploratory Structural Equation Modeling (ESEM) were conducted to evaluate the construct validity. The results of the CFA indicated that S-B chi(2)/df 1.90, CFI 0.95, TLI 0.94, RMSEA 0.06, and SRMR 0.05. The results of the ESEM indicated that S-B chi(2)/df 2.79, CFI 0.98, TLI 0.97, RMSEA 0.08, and SRMR 0.03. The item factor loadings ranged from 0.58 to 0.97 in the CFA and ranged from 0.45 to 0.82 in the ESEM. The findings indicated that the scale exhibited both discriminant and convergent validity, with CR and Cronbach's alpha being strong. An evaluation of the fit indices, item factor loadings, and inter-item relationships revealed that the CFA results were more consistent with the original structure of the scale. Based on all the findings, it can be concluded that the Turkish version of the scale is suitable for assessing the teacher identities of pre-service physical education teachers.Article The Relation Between Personality Traits and Chemotherapy Symptoms of Cancer Patients(Marmara Univ, Inst Health Sciences, 2025) Özkan, İlknur; Taylan, Seçil; Eroglu, Nermın; Kolac, NurcanObjective:This study is important as it is one of the first studies to evaluate the relationship between personality traits and symptoms. Methods:This study was planned as descriptive and cross-sectional. The study was conducted with 468 patients who were voluntary to participate in the study and applied to the chemotherapy unit in a private oncology hospital in Istanbul between January-June 2019. Data were obtained using the Patient Information Form, Chemotherapy Symptom Assessment Scale (C-SAS), and Big Five Inventory. Results:The data were evaluated on computer environment. The mean age of the patients was 59.46±11.78 years, 59.8% are female, 35.7% were secondary school graduate, and 36.5% were housewives.When the mean scores of the big five inventory were examined, it was found that the mean scores were 29.72±3.38 in Extraversion subscale, 28.31±5.62 in Agreeableness subscale, 27.14±4.44 in Conscientiousness subscale, 25.26±3.31 in Neuroticism subscale, and 28.31±5.62 in Openness subscale. One-unit increase in neuroticism was determined to increase post-treatment nausea by 1.14 times, diarrhea by 1.28 times, change in sexual life by 1.14 times, feeling pessimistic and sad by 1.071 times, and feeling anxious and distressed by 1.08 times. Conclusion:It was observed that the personality traits of cancer patients were correlated with the symptoms they experienced related to chemotherapy and the symptoms decreased with the increase of openness, extraversion, agreeableness and conscientiousness characteristics and the symptoms increased with the increase of neuroticism characteristic.These results indicated that healthcare professionals should consider personnel characteristics of cancer patients while evaluating the symptoms they experienced and providing care.Article Measuring Narcissism in Cyberspace: Psychometric Evaluation of the Turkish Online Narcissism Personality Inventory (ONPI)(Istanbul Univ, Methodology & Sociology Research Center, 2025) Aytac, Sevinc Serpil; Bilir, Kadriye Burcu Ongen; Aydin, Gulden Cetin; Yuzaki, EnginThe widespread use of digital technologies and the increasing prevalence of internet usage in a globalized world have significantly influenced individuals' personality traits. In particular, the restriction of face-to-face interactions during global pandemics has led individuals to maintain their lives in digital environments. These developments have made it increasingly important to investigate the relationship between internet use and personality characteristics. This study aims to adapt the Online Narcissistic Personality Inventory (ONPI), developed by Chou and Farn (2015) based on the 40-item Narcissistic Personality Inventory by Raskin and Terry (1988), into Turkish and to examine its psychometric properties. The study sample consists of 397 individuals from diverse age groups and socio-demographic backgrounds. Data was analyzed using SPSS 30 and AMOS 30 software. According to the results of the exploratory factor analysis, the item factor loadings ranged from 0.466 to 0.812, and the total variance explained by the scale was found to be 58.36%. Confirmatory factor analysis results (Chi-square/df:2.584; RMSEA:0.063; NFI: 0.84; IFI: 0.90; TLI: 0.87; CFI: 0.90; GFI: 0.90) indicated that the scale exhibits a five-factor structure with 27 items. The internal consistency of the scale was high, with a Cronbach's alpha coefficient of 0.93. Additionally, the item total correlations ranged between 0.326 and 0.700. The findings demonstrate that the Turkish adaptation of the Online Narcissistic Personality Inventory is a valid and reliable measurement tool.