WoS İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.14627/6
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Article Synergistic Effects of Amniotic Membrane and Human Milk Exosomes on Burn Wound Healing(Elsevier Sci Ltd, 2025) Isik, Ferda; Tufan, Elif; Sivas, Guzin Goksun; Ak, Esin; Muhan, Aleyna; Sener, Goksel; Tunali-Akbay, TugbaBackground: Thermal burns are one of the most common burns. Studies are ongoing to develop synthetic or biological wound dressings to ensure painless and scarless healing of burn wounds. Objectives: This study aimed to combine the human amniotic membrane with breast milk-based exosomes and investigate their effects on burn wound healing. Methods: 24 Wistar Albino rats weighing 200-250 g and of both genders were used. Rats were divided into control, burn, burn+human amniotic membrane (hAM) and burn+hAM+Exosomes (hAM+Exo) groups. Burn injury was induced by exposing the back of rats to 90 degrees C water for 10 s. Rats were treated with hAM and hAM+ Exo for seven days after injury. At the end of the 7th day, the skin samples were taken and analyzed biochemically and histologically. TNF-alpha, IL-1(i, type III collagen, malondialdehyde (MDA), glutathione (GSH), total protein, superoxide dismutase (SOD), and tissue factor (TF) activity were determined in skin samples. Results: In the burn group, skin TNF-alpha levels increased, IL-1(i and type III collagen levels decreased. Wound healing therapy reversed these results. In the hAM+Exo group, the TNF-alpha level was lower, and IL-1 beta and type III collagen levels were higher than in the hAM group. MDA and total protein levels increased, and GSH, tissue factor, and SOD activities decreased in the burn group. In hAM and hAM+Exo groups, MDA levels decreased, and GSH and SOD activity increased compared to the burn group. The GSH levels were significantly higher in the hAM+Exo group compared to the hAM group. Conclusion: In conclusion, combining exosomes and amniotic membrane induced changes consistent with better wound healing than amniotic membrane alone.Article Evaluation of the Neuroprotective Effects of Korean Ginseng Root Extract in an Experimental Model of Multiple Sclerosis(Marmara Univ, Fac Medicine, 2025) Donmez, Muhammet Oguzhan; Sener, Goksel; Akbay, Tugba Tunali; Sivas, Guzin Goksun; Akakin, Dilek; Unlu, Hilal; Goren, Mehmet Zafer; Goksun Sivas, Guzin; Tunali Akbay, TugbaObjective: Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system characterised by demyelination. The aim of this study was to evaluate the neuroprotective effects of Korean ginseng root extract (KGE) using a cuprizone-induced demyelination model. Materials and Methods: C57BL/6 mice were divided into control, demyelination and remyelination groups and each group was treated with KGE. Demyelination was induced with 0.2% cuprizone in the diet for four weeks. KGE (100 mg/kg) was administered by gavage during or after the cuprizone exposure. Body weight, food and water intake, and motor performance parameters were investigated. In addition, glutathione (GSH), glutathione-S-transferase (GST), superoxide dismutase (SOD) malondialdehyde (MDA), oligodendrocyte transcription factor-2 (OLIG2) and myelin basic protein (MBP) levels were measured in brain samples, while MBP and glial fibrillary acidic protein (GFAP) expression was assessed by immunohistochemistry and myelin status was examined using Luxol Fast Blue staining. Results: Korean ginseng root extract prevented myelin loss, promoted remyelination, and improved motor performance. It reduced oxidative stress by increasing GSH, GST, and SOD levels while decreasing MDA. KGE also suppressed demyelination by reducing astrogliosis and restoring OLIG2 and MBP levels. Conclusion: Korean ginseng root extract exhibits neuroprotective properties during demyelination and promotes remyelination, highlighting its therapeutic potential for MS.Article Citation - WoS: 5Citation - Scopus: 5Inhibitory Effect of Whey Protein Concentrate on Sars-Cov Furin Activity and Spike Protein-Ace2 Binding in Methotrexate-Induced Lung Damage(Wiley-hindawi, 2022) Tufan, Elif; Sivas, Guzin Goksun; Gurel-Gokmen, Begum; Yilmaz-Karaoglu, Sumeyye; Ercan, Dursun; Ozbeyli, Dilek; Tunali-Akbay, TugbaThis study aims to investigate the effects of whey proteins on SARS CoV-2 in methotrexate-induced lung tissue damage in rats. To determine the possible effects, rats were divided into four groups as control, control + whey, methotrexate (20 mg/kg, i.p.) and methotrexate + whey. Whey protein concentrate (2 g/kg, oral gavage) was administered for 10 days. Cytokine levels were measured and protein electrophoresis was carried out in serum samples. Lipid peroxidation, nitric oxide and glutathione level, and superoxide dismutase and glutathione S transferase activities were determined in lung samples. Inhibition of SARS CoV-2-targeted lung furin activity and SARS CoV-2 spike protein-angiotensin converting enzyme binding with whey protein concentrate were also measured in each group. In conclusion, whey protein concentrate improved methotrexate-induced lung damage and inhibited lung furin activity targeting SARS-CoV-2 S1/S2 site cleavage and SARS CoV-2 spike protein-angiotensin converting enzyme binding. Whey proteins are potential protective candidates that inhibit SARS CoV-2-related interactions, even in methotrexate-induced lung injury. Practical applications Whey proteins have anticarcinogenic, antihypertensive, antioxidant, antibacterial, antiviral, and immunomodulating properties due to the protein, bioactive peptide, and essential amino acid content. Methotrexate is a folate antagonist and inhibits cell proliferation and purine synthesis. The combined use of whey protein concentrate and methotrexate may be an alternative in the development of new strategies to the treatment approaches against COVID-19. In addition, according to the results of this study, it is thought that the protective effect of whey proteins in healthy conditions before encountering the SARS CoV-2 may be higher than those who have never used it.