WoS İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.14627/6
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Article Protective Effects of Panax Ginseng against Bisphenol A-Induced Testicular Toxicity in Rats(Springer Nature, 2026) Cesur, Yasemin; Cam, Kamil; Pazarbasi, Seren Ede; Dorucu, Dogancan; Sener, Goksel; Abas, Burcin Irem; Cevik, OzgeBisphenol A (BPA) is an endocrine-disrupting chemical known to cause testicular toxicity through oxidative stress and apoptosis. Panax ginseng (PG) is a natural product with anti-inflammatory effects. This study aimed to evaluate the protective effect of PG against BPA-induced testicular damage in rats. Thirty-two Wistar Albino rats aged 10-12 weeks were divided into four groups: Control, PG, BPA, and BPA + PG. BPA (50 mg/kg/day) and PG (100 mg/kg/day) were orally administered for 6 weeks. BPA significantly increased serum total oxidant status and decreased antioxidant status (p < 0.001, p < 0.001). Also, the levels of superoxide dismutase (an antioxidant enzyme) (p = 0.0005) and androgen receptor-mRNA (an androgen signaling marker) decreased (p = 0.014). However, caspase 3 (an apoptosis marker) (p = 0.0067), 8-hydroxy-2'-deoxyguanosine (a marker of oxidative DNA damage) (p < 0.001), and tumor necrosis factor-alpha (a proinflammatory cytokine) (p < 0.001) levels increased in testicular tissues. Rats treated with PG showed improvements in all oxidative and inflammatory markers and significantly restored androgen receptor expression. Histopathological examination revealed degeneration in seminiferous tubules and reduced spermatozoa in the BPA group, while the BPA + PG group showed marked improvement. These findings suggest that PG may alleviate oxidative stress-related testicular damage at both molecular and histological levels and may offer insights for future clinical studies.Article Montelukast Attenuates Abdominal Aortic Aneurysm in Rats: Anti-Inflammatory and Antioxidant Effects(Elsevier, 2026) Tekin, Gozde; Cevik, Ozge; Cetinel, Sule; Sener, Goksel; Kizilay, MehmetObjective: Oxidative stress and inflammation are widely recognized as central mechanisms in the pathogenesis of abdominal aortic aneurysm. This study sought to examine the potential protective properties of montelukast in a rat model of aortic aneurysm. Methods: Male Sprague-Dawley rats were randomly allocated into three experimental groups. Abdominal aortic aneurysm was induced using the calcium chloride (CaCl2) model, in which gauze soaked in 0.5 M CaCl2 was placed directly onto the adventitial surface of the infrarenal abdominal aorta for 15 minutes. After induction, the treatment group received daily intraperitoneal injections of montelukast (10 mg/kg) for 4 consecutive weeks. At the study end point, animals were euthanized, and infrarenal aortic tissues were harvested for biochemical and histological evaluations. Measured parameters included matrix metalloproteinase (MMP)-2 and MMP-9 expression, myeloperoxidase (MPO) activity, and 8-hydroxy-2 '-deoxyguanosine levels. Antioxidant capacity was assessed through superoxide dismutase (SOD) activity assays. Histopathological examinations were performed, and statistical analysis was conducted using GraphPad Prism v.5. Results: Exposure to CaCl2 triggered pronounced oxidative injury and inflammation, as evidenced by elevated 8-hydroxy-2 '-deoxyguanosine levels, increased MPO activity, reduced SOD activity, and upregulated MMP-2 and MMP-9 expression. Montelukast administration markedly attenuated these changes, normalizing oxidative and inflammatory markers while improving histopathological architecture. Conclusions: Montelukast effectively counteracted CaCl2-induced aortic damage. The protective effects of montelukast appear to be mediated through suppression of MMP activity, restoration of SOD levels, and reduction of MPO-driven oxidative injury. By mitigating both inflammatory and oxidative mechanisms, montelukast contributes to the preservation of aortic wall structure. Clinical Relevance: Abdominal aortic aneurysm remains a major vascular disorder without an effective pharmacological therapy to slow its progression. In this experimental study, montelukast, a leukotriene receptor antagonist widely used in asthma, attenuated abdominal aortic aneurysm formation in rats and was associated with increased superoxide dismutase activity, reduced myeloperoxidase levels, and suppressed matrix metalloproteinase activation. These combined antioxidant, anti-inflammatory, and matrix-stabilizing effects preserved aortic wall integrity. Given montelukast's established safety and clinical availability, these findings support its potential for future clinical investigation as a pharmacological approach to limit aneurysm progression. (JVS-Vascular Science 2026;7:100405.)Article In Vivo and In Silico Evaluation of the Effects of Parsley (Petroselinum Crispum L.) Extract on Small Intestinal Tissue in Scopolamine-Induced Cognitive Dysfunction Model(Wiley-VCH Verlag GmbH, 2025) Ertik, Onur; Sacan, Ozlem; Sener, Goksel; Pazarbasi, Seren Ede; Yanardag, RefiyeThe brain-small intestine connection has become important in neurodegenerative diseases in recent years. In particular, the discovery of the relationship between Alzheimer's disease (AD) and the small intestine and the examination of the effects of AD on this tissue are important in this respect. Our study aimed to understand the effects of the experimentally created AD model in rats on the small intestinal tissue and the protective effect of the extract prepared from parsley leaves (PE). The experimental animals were divided into four groups in the study; Control, Scopolamine (Scop), Scop + PE and Scop + Galantamine (GAL). Oxidative stress parameters and activities of some important enzymes were examined in small intestinal tissues taken as a result of the experimental protocol. Additionally, in silico studies were carried out for bioactive molecules found in parsley leaves using data obtained from in vivo enzyme activity results. It was found that parameters examined for the damaged group, Scop, were reversed by PE and GAL treatment. As a result of in silico studies, it was determined that oxypeucedanin and phylloquinone had higher binding affinity than rutin for acetylcholinesterase (AChE). It has been observed that oxidative damage in the small intestine due to AD can be treated by the PE.Article The Therapeutic Role of Ginseng in Promoting Hippocampal Neurogenesis and Ameliorating Cognitive Function Following Whole Brain Radiotherapy in Rats(Springer/Plenum Publishers, 2025) Sahin, Sevim; Bayindir, Nihan; Ertas, Busra; Ceylan, Cemile; Elibol, Birsen; Ozkan, Alper; Sener, GokselWhole-brain radiotherapy (WBRT) is a prevalent technique for managing multiple intracranial metastases, however, the cognitive damage in long-term survivors due to WBTR is a critical concern that impacts patients' quality of life. Panax ginseng, a bioactive compound recognized for its neuroprotective benefits, also enhances cognitive functions, including memory and learning. This study aims to examine the potential protective effects of Panax ginseng supplementation on cognitive dysfunction and the levels of neurogenesis-related proteins in the hippocampus of rats that underwent WBRT, which was delivered as 3 fractions of 6 Gy (total dose 18 Gy) using a linear accelerator. Thirty-six male Sprague-Dawley rats were divided into three groups: radiation, ginseng treatment, and control. After 60 days of Panax ginseng administration (100 mg/kg), behavior tests (Morris water maze and novel object recognition) were performed, followed by western blot analysis of the hippocampus. Results indicated that Panax ginseng supplementation ameliorated radiation-induced cognitive impairments. Additionally, western blot analyses revealed that Panax ginseng promoted neuronal recovery and neuroplasticity processes in the hippocampus, simultaneously exhibiting a neuroprotective mechanism by reducing apoptosis and neurotoxicity markers. Panax ginseng ameliorates cognitive dysfunction after WBRT by enhancing neurogenesis and diminishing cell death in the hippocampus.Article Protective Effect of Artichoke (Cynara scolymus L.) Leaf and Receptaculum Extracts Against Hepatic Encephalopathy in Bile Duct Ligated Rats(Springer/Plenum Publishers, 2025) Kam, Ozkan; Bebitoglu, Berna Terzioglu; Sener, Goksel; Oguz, Elif; Erdogan, Nurettin Fatih; Kilickap, Andac; Hatiboglu, NebileHepatic encephalopathy (HE), complication of liver dysfunction, leads to neurocognitive impairments. Artichoke (Cynara scolymus L.) has been traditionally used for its antioxidant, anti-inflammatory and hepatoprotective properties. This study evaluates artichoke leaf and receptaculum extracts in cholestasis and HE in a rat model. Wistar rats were divided into 6 groups: sham-control, bile duct ligation (BDL), and BDL with low/high-dose leaf or receptaculum extracts. After BDL, physiological saline and extracts (250/500 mg/kg) were administered orally for 28 days. Cognitive activity was evaluated using Morris water maze and novel object recognition tests on day 28. Artichoke extract regulated liver enzymes and bilirubin at high-doses and significantly increased antioxidant enzyme activities reduced by BDL. Elevated 8-Hydroxyguanosine (8-OHdG) levels decreased in liver and brain tissues. Similarly, artichoke extracts reduced cytokine and hydroxyproline (HP) levels elevated by cholestasis. Following BDL, Na+/K+-ATPase levels in brain and liver tissues decreased, while artichoke extract reversed this. Artichoke, particularly high-dose receptaculum, improved impaired performance and increased time in the target quadrant after BDL. Both artichoke leaf and receptaculum extracts improved recognition. Artichoke treatments, especially high-dose receptaculum, reduced hepatic and neuronal damage and improved histological appearance. These findings highlight the therapeutic potential of artichoke extracts for liver fibrosis and related neurocognitive disorders.Article Synergistic Effects of Amniotic Membrane and Human Milk Exosomes on Burn Wound Healing(Elsevier Sci Ltd, 2025) Isik, Ferda; Tufan, Elif; Sivas, Guzin Goksun; Ak, Esin; Muhan, Aleyna; Sener, Goksel; Tunali-Akbay, TugbaBackground: Thermal burns are one of the most common burns. Studies are ongoing to develop synthetic or biological wound dressings to ensure painless and scarless healing of burn wounds. Objectives: This study aimed to combine the human amniotic membrane with breast milk-based exosomes and investigate their effects on burn wound healing. Methods: 24 Wistar Albino rats weighing 200-250 g and of both genders were used. Rats were divided into control, burn, burn+human amniotic membrane (hAM) and burn+hAM+Exosomes (hAM+Exo) groups. Burn injury was induced by exposing the back of rats to 90 degrees C water for 10 s. Rats were treated with hAM and hAM+ Exo for seven days after injury. At the end of the 7th day, the skin samples were taken and analyzed biochemically and histologically. TNF-alpha, IL-1(i, type III collagen, malondialdehyde (MDA), glutathione (GSH), total protein, superoxide dismutase (SOD), and tissue factor (TF) activity were determined in skin samples. Results: In the burn group, skin TNF-alpha levels increased, IL-1(i and type III collagen levels decreased. Wound healing therapy reversed these results. In the hAM+Exo group, the TNF-alpha level was lower, and IL-1 beta and type III collagen levels were higher than in the hAM group. MDA and total protein levels increased, and GSH, tissue factor, and SOD activities decreased in the burn group. In hAM and hAM+Exo groups, MDA levels decreased, and GSH and SOD activity increased compared to the burn group. The GSH levels were significantly higher in the hAM+Exo group compared to the hAM group. Conclusion: In conclusion, combining exosomes and amniotic membrane induced changes consistent with better wound healing than amniotic membrane alone.Article Protective Effects of Cuscuta Sp. Against Cardiorenal Injury in Bile Duct-Ligated Rats(Istanbul Univ, 2025) Hatipoglu, Bilge Nur; Ozbeyli, Dilek; Sen, Ali; Cevik, Ozge; Ercan, Feriha; Albayrak, Omercan; Sener, GokselObjective: Bile duct ligation (BDL) obstructs bile flow, resulting in bile and toxic substances buildup that causes liver damage. This study investigated the protective effects of Cuscuta sp. methanol extract (CUS) against cardiorenal injury in bile duct-ligated rats. Materials and Methods: Rats were categorised into four groups: Control (C), CUS, BDL, and BDL+CUS. The C and BDL groups received saline, whereas the other groups received oral 250 mg/kg CUS. After 28 days, blood, kidney, and heart tissue samples were collected for biochemical and histological analyses. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), direct bilirubin (DB), and total bilirubin (TB) levels were analysed to determine liver function, while Transforming growth factor-beta (TGF-beta) and hydroxyproline (HYP) levels were evaluated for fibrosis, and 8-hydroxy-2-deoxyguanosine (8-OHdG) levels alongside Na+/K+-ATPase activity were analysed to assess oxidative stress and membrane injury in the heart and renal tissues. Results: AST, ALT, DB, and TB levels were significantly elevated in the BDL group compared with the C group; however, the levels were distinctly lower in the BDL+CUS group than in the BDL group. Additionally, in both tissues, TGF-beta, HYP, and 8-OHdG levels were higher in the BDL group than in the C group, but decreased in the BDL+CUS group, with Na+/K+-ATPase activity being lower in BDL group compared with the C group and significantly increased in BDL+CUS group. Conclusion: CUS has protective effects against oxidative damage and offers antioxidant and anti-inflammatory benefits against cholestasis-induced tissue injury.Article Evaluation of the Neuroprotective Effects of Korean Ginseng Root Extract in an Experimental Model of Multiple Sclerosis(Marmara Univ, Fac Medicine, 2025) Donmez, Muhammet Oguzhan; Sener, Goksel; Akbay, Tugba Tunali; Sivas, Guzin Goksun; Akakin, Dilek; Unlu, Hilal; Goren, Mehmet Zafer; Goksun Sivas, Guzin; Tunali Akbay, TugbaObjective: Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system characterised by demyelination. The aim of this study was to evaluate the neuroprotective effects of Korean ginseng root extract (KGE) using a cuprizone-induced demyelination model. Materials and Methods: C57BL/6 mice were divided into control, demyelination and remyelination groups and each group was treated with KGE. Demyelination was induced with 0.2% cuprizone in the diet for four weeks. KGE (100 mg/kg) was administered by gavage during or after the cuprizone exposure. Body weight, food and water intake, and motor performance parameters were investigated. In addition, glutathione (GSH), glutathione-S-transferase (GST), superoxide dismutase (SOD) malondialdehyde (MDA), oligodendrocyte transcription factor-2 (OLIG2) and myelin basic protein (MBP) levels were measured in brain samples, while MBP and glial fibrillary acidic protein (GFAP) expression was assessed by immunohistochemistry and myelin status was examined using Luxol Fast Blue staining. Results: Korean ginseng root extract prevented myelin loss, promoted remyelination, and improved motor performance. It reduced oxidative stress by increasing GSH, GST, and SOD levels while decreasing MDA. KGE also suppressed demyelination by reducing astrogliosis and restoring OLIG2 and MBP levels. Conclusion: Korean ginseng root extract exhibits neuroprotective properties during demyelination and promotes remyelination, highlighting its therapeutic potential for MS.Article NADPH Oxidase-2 Inhibitor Apocynin Attenuates High-Fat Diet-Induced Kidney and Bladder Injury(Marmara Univ, Fac Medicine, 2025) Kanpalta Mustafaoglu, Fatma; Ertas, Busra; Sener, Goksel; Ercan, FerihaObjective: This study aimed to evaluate the effects of NADPH oxidase-2 (NOX-2) inhibitor apocynin (APC) on high-fat diet (HFD)induced renal and bladder injury. Materials and Methods: Wistar albino rats were divided into 4 groups: Control, HFD, HFD+dimetyl sulfoxide (DMSO), and HFD+APC. Rats in HFD, HFD+DMSO, and HFD+APC groups were fed with HFD for sixteen weeks. In the last 4 weeks of the experiment, either DMSO or APC (25 mg/kg, dissolved in DMSO) was applied to the HFD+DMSO or HFD+APC groups. Lipid profiles and leptin values were measured in blood serum. Renal and bladder oxidant/antioxidant parameters, histological changes in the tissues, NOX-2-, nuclear factor kappa B (NF-& kgreen;B)-immunopositive and apoptotic cells were evaluated. Results: At the end of the experiment, leptin, cholesterol, and triglyceride levels were higher and high-density lipoprotein levels were lower in the HFD and HFD+DMSO groups compared to controls. In these experimental groups, an increase in malondialdehyde, 8-hydroxy-deoxyguanosine and myeloperoxidase levels and a decrease in glutathione levels, as well as an increase in collagen, NOX-2-and NF & kgreen;-B-immunopositive and apoptotic cells were found. Also, a deterioration in kidney and bladder morphology was observed. All these biochemical and histopathological findings improved in the HFD+APC group. Conclusion: High-fed diet causes renal and bladder injury by increasing NOX-2 activity and inflammation via oxidative stress. APC might alleviate tissue injury by inhibiting oxidative stress.Article Citation - WoS: 3Citation - Scopus: 2Morphological and Biochemical Investigation of the Protective Effects of Panax Ginseng on Methotrexate-Induced Testicular Damage(Istanbul Univ, 2023) Karakaya-Cimen, Fatma Bedia; Macit, Caglar; Sivas, Guzin Goksun; Akbay, Tugba Tunali; Sener, Goksel; Ercan, Feriha; Cımen, Fatma Bedia KarakayaObjective: Methotrexate (MTX) is a chemotherapeutic agent that causes testicular toxicity used in the cure of various types of cancer. The anti-oxidant and anti-cancer effects of Panax ginseng (PxG) have been reported in both experimental and clinical studies. This study aims to examine the healing effect of PxG on testicular damage induced by MTX. Materials and Methods: Sprague Dawley male rats (8-week-olds) were used in the study. A single dose ofMTXdissolved in saline (20 mg/kg) was given to MTX and MTX+PxG groups by intraperitoneal injection. PxG dissolved in saline (100 mg/kg) was given by orogastric gavage once a day for 5 days to the MTX+PxG group. Saline was given to the control and MTX groups orally during the experiments. After decapitation, the testis sampleswere obtained. Seminiferous tubules and basement membranewere evaluated histopathologically. Seminiferous tubule diameter and germinal epithelium thickness were measured. Furthermore, oxidative stress parameters such as malondialdehyde, glutathione, superoxide dismutase, and glutathione-S-transferase were measured. Results: MTX treatment caused seminiferous tubule degeneration with a decrease in Johnsen's score, the seminiferous tubule's diameter, and the germinal epithelium's thickness. Parallel with the histopathological results increased testicular oxidative stress with an increase in malondialdehyde level and a decrease of endogenous anti-oxidant activity with a decrease in glutathione level and glutathione-S-transferase and superoxide dismutase activities. PxG treatment improved these histological and biochemical parameters in MTX-induced testis cytotoxicity. Conclusion: MTX treatment causes testicular damage via the oxidative processes. PxG treatment ameliorates MTX-induced testicular damage by inhibiting oxidative stress.