WoS İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.14627/6
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Article Dodder (Cuscuta sp.) Extract Prevents Cognitive Deficits in a Rat Model of Hepatic Encephalopathy(Kare Publ, 2024) Cevik, Ozge; Sen, Ali; Şener, Göksel; Ercan, Feriha; Doğan, Ahmet; Özbeyli, Dilek; Hatipoğlu, Bilge Nur; Albayrak, Omercan; Koyuncuoglu, TurkanOBJECTIVE: In our study, the protective effect of dodder plant extract against encephalopathy induced by cholestatic liver disease model was investigated. METHODS: Spraque Dawley rats were used in the study. For the cholestatic liver disease model, the bile duct ligation (BDL) was applied. The groups were determined as control, Cuscuta sp. (CUS), BDL and BDL + CUS. Double ligation was performed in the bile duct in the BDL groups. For the applications, saline (SF) was administered to the control and BDL groups for 28 days while 250 mg/ kg of Cuscuta sp. extract was given by oral gavage to the CUS and BDL + CUS groups. At the end of the experiment, cognitive eval- uations were made by applying new object recognition and Morris water maze tests. After these tests, blood-brain barrier (BBB) measurements were made in half of the groups. In the other half of the groups, brain tissue samples were taken by decapitation and transforming growth factor-beta (TGF-β), 8-hydroxydeoxyguanosine (8-OHdG) and sodium-potassium adenosine triphospha- tase (Na+/K+-ATPase) measurements were made in the tissues. Histological examinations of the tissues were also performed. RESULTS: Cognitive performance was low, and BBB permeability was found to be increased in the group with bile duct liga- tion. In addition, TGF-β and 8-OHdG levels were increased in tissues, while Na+/K+-ATPase enzyme activity was suppressed. Treatment with Cuscuta sp. increased cognitive performance and decreased BBB permeability. Other biochemical parameters examined were significantly (p<0.05–0.001) reversed and supported by histological findings. CONCLUSION: Our findings in the study suggest that dodder plant may be beneficial for the protection of cognitive perfor- mance and brain tissue in encephalopathy caused by cholestasis. Keywords: Cuscuta sp.; cholestasis; encephalopathy; fibrosis.Article Citation - WoS: 2The Effect of <i>myrtus Communis</I> L. Extract on Nephrolithiasis Model in Rats(Kare Publ, 2024) Ertas, Busra; Dorucu, Dogancan; Gulerturk, Oznur; Sen, Ali; Cevik, Ozge; Cetinel, Sule; Sener, GokselOBJECTIVE: Nephrolithiasis is a common urological disease that can lead to renal failure. Oxidative stress has been shown to be a contributing factor for nephrolithiasis and many agents have been studied to prevent and treat oxidative stress-related nephrolithiasis and renal damage. Myrtus communis (MC) extract has been shown to be an important antioxidant in different animal models. In this study, MC extract was administered preventively or therapeutically to rats with kidney stones, and its effectiveness was investigated. METHODS: Wistar albino rats were divided into four groups (n=8); control (C), ethylene glycol (EG), EG+preventive MC, and EG+curative MC groups. The nephrolithiasis model was created by adding 0.75% EG to drinking water for 8 weeks. Ultimately, 24-hour urine was collected to measure calcium, citrate, and creatinine levels. After decapitation, kidney tissues were harvested for histological analyses, measurement of osteopontin and 8-hydroxydeoxyguanosine (8-OHdG) levels, and N-acetyl-beta-glucosaminidase (NAG), myeloperoxidase (MPO) and caspase-3 activities. RESULTS: In 24-hour urine samples, calcium, citrate and creatinine levels were decreased in the EG group, while oxalate levels were increased and in treatment groups these parameters returned to control levels. MPO, 8-OHdG, caspase-3 and NAG activity were significantly increased in tissue and these changes were reversed in both MC groups. Histological findings also supported the biochemical parameters. CONCLUSION: MC can reduce oxidative stress and histopathological changes in kidney tissues in rat nephrolithiasis model when used as either a preventive or therapeutic agent. If supported with further clinical trials, MC might have clinical implications in preventing oxidative renal cell injury and ultimately kidney stone formation.Article Citation - WoS: 6Citation - Scopus: 6Myrtle Improves Renovascular Hypertension-Induced Oxidative Damage in Heart, Kidney, and Aortic Tissue(Springer, 2022) Arslan, Sule; Ozcan, Ozan; Gurel-Gokmen, Begum; Cevikelli-Yakut, Zatiye Ayca; Saygi, Halil Ibrahim; Sen, Ali; Tunali-Akbay, TugbaRenovascular hypertension is defined as the reduction in renal perfusion resulting in sustained hypertension. This study aims to investigate the possible effects of myrtle leaf extract (Myrtus communis L.) on, heart, kidney and aorta tissues in the experimental renovascular hypertension (RVH). 32 male Wistar Albino rats were divided into four groups as control, hypertension, hypertension+ramipril, and hypertension+myrtle leaf extract treatment groups. RVH model was induced by Goldblatt's 2-kidney 1-clip method. 12 weeks later than the treatment blood pressures were recorded and oxidant and antioxidant parameters, tissue factor activity, and histological analysis were determined in the kidney, heart, and aortic tissues. The blood pressure levels of the hypertension group significantly increased compared to the control group. Administration of myrtle leaf extract and ramipril significantly decreased the increased blood pressure. In the hypertension group, oxidative damage increased in the kidney, heart, and aorta tissues. In the histological evaluation of tissues in RVH, heart muscle fibres degenerated. Bowman capsule and glomeruli dilated and tubules damaged in the kidney. Myrtle leaf extract administration regenerated the damages and degenerations. The administration of myrtle leaf extract restored the impaired oxidant-antioxidant balance in the heart, kidney and aorta tissues of hypertensive rats. Myrtle leaf extract can be considered as an alternative antihypertensive treatment target in the prevention of oxidative stress-induced damage in renovascular hypertension.