WoS İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.14627/6
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Article Myrtus Communis Ameliorates Ionizing Radiation-Induced Cardiopulmonary Injury in Rats: Trod-Grog Study(Keai Publishing Ltd, 2026) Aytekin, Aynur; Isci, Oguzhan; Ozyilmaz, Nagehan; Karaoglu, Sumeyye Yilmaz; Ertas, Busra; Sen, Ali; Atasoy, Beste Melek; Yılmaz Karaoğlu, SümeyyeObjectives: Ionizing radiation (IR), widely used in diagnostic and therapeutic procedures, can damage vital organs such as the heart and lungs through oxidative stress. This study aims to assess the potential radioprotective effect of Myrtus communis (MC) against cardiopulmonary injury. Methods: Thirty female rats were divided into four groups. Control (C) and IR (R) groups received oral saline. The treatment (R+MC) and pretreatment (R+preMC) groups received MC (100 mg/kg) for 4 days (starting on the day of IR) and 8 days (starting 4 days before IR), respectively. All IR-exposed groups (R, R+MC, R+preMC) received a single 10 Gy whole-body irradiation. Histopathological changes were assessed by hematoxylin-eosin staining, while oxidative stress was evaluated by measuring malondialdehyde (MDA), glutathione (GSH), myeloperoxidase (MPO), nitric oxide (NO), superoxide dismutase (SOD), glutathione S-transferase (GST), and tissue factor activity (TFa) levels. Protein profiles in tissues were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Results: Histopathologically, MC reduced alveolar and cardiomyocyte damage in both R+MC and R+preMC groups. IR increased all oxidative stress markers and decreased antioxidant parameters in heart and lung tissues (p < 0.05-0.001). Both MC treatment and pretreatment reversed these effects, significantly reducing oxidative/inflammatory markers and restoring antioxidant enzyme activities (p < 0.05-0.001). The R+preMC group demonstrated a stronger protective effect than the R+MC group. Conclusion: Our study shows that MC has a radioprotective effect on the cardiopulmonary system by decreasing oxidative damage. MC appears to be a promising natural compound for advanced radioprotection research, and further molecular and clinical studies could clarify its mechanisms and potential applications<bold>.</bold>Article Gastroprotective Role of Myrtus Communis in a Dual-Condition Model Combining Diabetes and Postmenopausal Rats: Comparable Outcomes to Estrogen Therapy(Cukurova Univ, Fac. Medicine, 2025) Dagsuyu, Eda; Kadioglu-Yaman, Beril; Sen, Ali; Sener, Goksel; Yanardag, RefiyePurpose: Hormonal changes during menopause, especially the decline in estrogen and progesterone levels, can impair gastrointestinal function by slowing digestion. This study aimed to evaluate the protective effects of Myrtus communis subsp. communis (MC) extract on gastric tissue in a postmenopausal diabetic rat model and to compare its efficacy with estrogen (E2) treatment. Materials and Methods: Female Sprague-Dawley rats were divided into six groups: control (C); ovariectomy (OVX); diabetes (D); ovariectomy and diabetes (OVX+D); ovariectomy, diabetes and estrogen (OVX+D+E2); ovariectomy, diabetes and MC (OVX+D+MC). OVX groups had bilateral ovariectomy utilizing the double dorsolateral method. After a seven-day recovery, diabetes was induced using streptozotocin. OVX+D+E2 and OVX+D+MC groups were treated with 10 mu g/kg s.c. E2 and 100 mg/kg p.o. MC extract, respectively, for four weeks. Stomach tissues were analyzed for biochemical parameters. Results: The OVX, D, and OVX+D showed significant reductions in antioxidant enzyme activities and glutathione levels, and increases in oxidative stress markers and lipid peroxidation compared to the C group. E2 and MC treatments effectively mitigated these alterations, with MC showing comparable or superior effects to E2 in many parameters. MC treatment significantly improved blood glucose levels (-60% reduction in the OVX+D), stomach Na+/K+-ATPase activity (-110% increase in the OVX+D), and glycoprotein component levels. Conclusion: MC extract exhibits potent antioxidant and gastroprotective effects in postmenopausal diabetic rats, comparable to E2 treatment. These findings highlight its potential as a natural therapeutic agent for managing menopause-and diabetes-associated gastric complications.Article Citation - WoS: 2The Effect of <i>myrtus Communis</I> L. Extract on Nephrolithiasis Model in Rats(Kare Publ, 2024) Ertas, Busra; Dorucu, Dogancan; Gulerturk, Oznur; Sen, Ali; Cevik, Ozge; Cetinel, Sule; Sener, GokselOBJECTIVE: Nephrolithiasis is a common urological disease that can lead to renal failure. Oxidative stress has been shown to be a contributing factor for nephrolithiasis and many agents have been studied to prevent and treat oxidative stress-related nephrolithiasis and renal damage. Myrtus communis (MC) extract has been shown to be an important antioxidant in different animal models. In this study, MC extract was administered preventively or therapeutically to rats with kidney stones, and its effectiveness was investigated. METHODS: Wistar albino rats were divided into four groups (n=8); control (C), ethylene glycol (EG), EG+preventive MC, and EG+curative MC groups. The nephrolithiasis model was created by adding 0.75% EG to drinking water for 8 weeks. Ultimately, 24-hour urine was collected to measure calcium, citrate, and creatinine levels. After decapitation, kidney tissues were harvested for histological analyses, measurement of osteopontin and 8-hydroxydeoxyguanosine (8-OHdG) levels, and N-acetyl-beta-glucosaminidase (NAG), myeloperoxidase (MPO) and caspase-3 activities. RESULTS: In 24-hour urine samples, calcium, citrate and creatinine levels were decreased in the EG group, while oxalate levels were increased and in treatment groups these parameters returned to control levels. MPO, 8-OHdG, caspase-3 and NAG activity were significantly increased in tissue and these changes were reversed in both MC groups. Histological findings also supported the biochemical parameters. CONCLUSION: MC can reduce oxidative stress and histopathological changes in kidney tissues in rat nephrolithiasis model when used as either a preventive or therapeutic agent. If supported with further clinical trials, MC might have clinical implications in preventing oxidative renal cell injury and ultimately kidney stone formation.Article Citation - WoS: 1Histological and Biochemical Effects of an Ethanolic Extract of <i>myrtus Communis</I> Leaf on the Pancreases of Rats Fed High Fat Diets(Taylor & Francis Ltd, 2024) Kabatas, Gul Sinemcan; Ertas, Busra; Sen, Ali; Sener, Goksel; Ercan, Feriha; Akakin, DilekWe investigated the effects of an ethanolic extract of Myrtus communis subsp. communis (MC) leaves on the pancreases of rats fed with a high fat diet (HFD). Wistar albino rats were fed either with standard lab chow (Control group) or with a 45% fat diet (HFD and HFD+MC groups) for 4 months, with the MC extract (100 mg/kg) being administered by orogastric gavage to rats in the HFD+MC group during the last month. Blood and pancreas samples were collected from all experimental groups at the end of the study. Insulin and leptin levels, and the lipid profile, were analyzed in the blood serum. Pancreatic injury was assessed histologically. Insulin, nuclear factor kappa beta (NF-kappa B), and alpha-smooth muscle actin (alpha-SMA) were assessed using immunohistochemistry. Apoptosis was assessed using terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) immunohistochemistry. In addition, oxidant/antioxidant activity was analyzed by biochemical methods. Increased body weight, serum insulin and leptin levels, blood glucose level and pancreatic tissue malondialdehyde (MDA), 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels, myeloperoxidase (MPO) activity and decreased tissue glutathione (GSH) level were observed in the HFD group compared to the Control group, in addition to dyslipidemia. An increased histopathological damage score, pancreatic islet area, insulin, TUNEL, NF-kappa B and alpha-SMA immunoreactivity were seen in animals from the HFD group compared to the Control group. However, such pathological changes were reduced in the HFD+MC group. Our data indicate further investigation of MC extract as a therapeutic adjuvant for HFD-induced pancreatic injury, acting via anti-inflammatory and antioxidant mechanisms, is worth carrying out.Article Citation - WoS: 1Ameliorative Effects of Myrtus Communis L. Extract Involving the Inhibition of Oxidative Stress on High Fat Diet-Induced Testis Damage in Rats(Taylor & Francis Ltd, 2024) Coskunlu, Busra; Koroglu, M. Kutay; Hersek, Irem; Ertas, Busra; Sen, Ali; Sener, Goksel; Ercan, FerihaThe possible protective effects of Myrtus communis L. (MC) extract on a high fat diet (HFD)-induced testicular injury in a rat model were investigated using histological and biochemical methods. Wistar albino rats were divided into three groups: a standard diet control group; a HFD group; and an HFD+MC group. The HFD and HFD+MC groups were fed with a HFD for 16 weeks. MC extract (100 mg/kg) was given orally five days a week to the rats in the HFD+MC group during the last four weeks of the experiment. Leptin, triglyceride, high-density lipoproteins, cholesterol, estrogen, testosterone, LH and FSH were analyzed in blood serum. Sperm parameters were evaluated from the epididymis. Testicular morphology, proliferative, apoptotic and NADPH oxidase-2 (NOX2)-positive cells were evaluated histologically. Testicular oxidative stress parameters were analyzed biochemically. In the HFD group, lipid and hormone profiles were changed, abnormal spermatozoa, degenerated seminiferous tubules with apoptotic and NOX2-positive cells were increased in number, and sperm motility and germinal proliferative cells decreased compared to the control group. Moreover, testicular malondialdehyde, 8-hydroxy-2-deoxyguanosine and myeloperoxidase levels increased, whereas glutathione and superoxide dismutase levels decreased in the HFD group compared to the control group. All these histological and biochemical features were ameliorated by MC treatment of HFD-fed rats. In conclusion, HFD caused alterations in sperm parameters and testicular morphology by increasing oxidative damage and apoptosis. MC extract may have potential protective effects by inhibiting oxidative damage.Article Citation - WoS: 5Citation - Scopus: 7Bitter Melon (Momordica charantia) Fruit Extract Ameliorates Methotrexate-Induced Reproductive Toxicity in Male Rats(Marmara University, 2021) Kanpalta, Fatma; Özbeyli, Dilek; Sen, Ali; Cevik, O. Dağdeviren; Şener, Göksel; Ercan, FerihaObjective: Methotrexate (MTX) is a drug commonly used for the treatment of malign neoplastic and inflammatory diseases. Anti-oxidant and anti-inflammatory effects of bitter melon (BM) were reported. The aim of this study was to examine the effects of BM fruit extract on MTX-induced testicular and epididymal damage. Materials and Methods: Sprague Dawley male rats were divided into three groups (n=8) as control, MTX and MTX+BM. A single dose of MTX (20 mg/kg) was injected intraperitoneally to the MTX and MTX+BM groups. BM fruit extract (600 mg/kg) was applied to the MTX+BM group orally for 5 days. Testes were examined for general histopathology, proliferating and apoptotic cells. The epididymis samples were used for the evaluation of sperm morphology. Oxidative and inflammatory markers were analysed biochemically. Results: Increased abnormal spermatozoa, degenerated seminiferous tubules with increased apoptotic cells and decreased proliferative cells were observed in the MTX group. TNF-α, IL-1β, 8-hydroxy-2-deoxyguanosine and caspase-3 levels increased, superoxide dismutase and catalase levels decreased in both testis and epididymis samples. All these histological and biochemical parameters were ameliorated in the MTX+BM group. Conclusion: Methotrexate causes testis damage by decreasing spermatogenic cells and increasing apoptosis through oxidative stress and inflammation. BM extract improves testis and epididymis damage with its possible anti-oxidant and anti-inflammatory effects. © 2025 Elsevier B.V., All rights reserved.Article Citation - WoS: 1The Protective Effects Of<i> Momordica</I><i> Charantia</I> Fruit Extract in Methotrexate Induced Liver Damage in Rats(Galenos Publ House, 2022) Ozbeyli, Dilek; Sen, Ali; Cevik, Ozge; Erdogan, Omer; Kaya, Ozlem Tugce Cilingir; Ede, Seren; Sener, Goksel; Ede-pazarbasi, Seren; Cilingir-kaya, Ozlem TugceBACKGROUND/AIMS: Methotrexate (MTX), a cytotoxic therapeutic agent, is used for the cure of malignancies and rheumatologic disorders. However, the significant side effects of MTX limits its use. In this study, we aim to assess the hepatoprotective properties of Momordica charantia (MC) against MTX-induced liver damaged in rats.MATERIALS AND METHODS: Following one dose of MTX (20 mg/kg), the rats were given either distilled water or MC extract (300 mg/kg, po) for 5 days. After the dissection of the rats, the liver was removed to analyse tumour necrosis factor -a (TNF-a), interleukin-113 (IL-113), transforming growth factor 13 (TGF-13) and 8-hydroxy-2'-deoxy-guanosine (8-OhdG) levels and superoxide dismutase (SOD), catalase (CAT), and caspase-3 activities. The tissues were also examined histopathologically.RESULTS: The hepatic TNF-a, IL-113, TGF-13, 8-OhdG levels, and Caspase-3 activity in the MTX group were found to be significantly increased compared to the control group. However, MC extract was able to significantly decrease TNF-a, TGF-13, 8-OhdG levels, and Caspase-3 activity. Also, both the SOD and CAT activity of the MTX group decreased compared to the control group. Although only the SOD levels elevated significantly with MC treatment, the SOD and CAT activities of the MC treated group were similar to the control group. Supporting these biochemical parameters, MTX-induced histologic alterations in the liver were also ameliorated via MC treatment.CONCLUSION: Our results demonstrated that MC has a protective role against MTX-induced hepatic tissue injury by reducing apoptosis, oxidative damage, and the expression of pro-inflammatory cytokines.Article Citation - WoS: 6Citation - Scopus: 6Myrtle Improves Renovascular Hypertension-Induced Oxidative Damage in Heart, Kidney, and Aortic Tissue(Springer, 2022) Arslan, Sule; Ozcan, Ozan; Gurel-Gokmen, Begum; Cevikelli-Yakut, Zatiye Ayca; Saygi, Halil Ibrahim; Sen, Ali; Tunali-Akbay, TugbaRenovascular hypertension is defined as the reduction in renal perfusion resulting in sustained hypertension. This study aims to investigate the possible effects of myrtle leaf extract (Myrtus communis L.) on, heart, kidney and aorta tissues in the experimental renovascular hypertension (RVH). 32 male Wistar Albino rats were divided into four groups as control, hypertension, hypertension+ramipril, and hypertension+myrtle leaf extract treatment groups. RVH model was induced by Goldblatt's 2-kidney 1-clip method. 12 weeks later than the treatment blood pressures were recorded and oxidant and antioxidant parameters, tissue factor activity, and histological analysis were determined in the kidney, heart, and aortic tissues. The blood pressure levels of the hypertension group significantly increased compared to the control group. Administration of myrtle leaf extract and ramipril significantly decreased the increased blood pressure. In the hypertension group, oxidative damage increased in the kidney, heart, and aorta tissues. In the histological evaluation of tissues in RVH, heart muscle fibres degenerated. Bowman capsule and glomeruli dilated and tubules damaged in the kidney. Myrtle leaf extract administration regenerated the damages and degenerations. The administration of myrtle leaf extract restored the impaired oxidant-antioxidant balance in the heart, kidney and aorta tissues of hypertensive rats. Myrtle leaf extract can be considered as an alternative antihypertensive treatment target in the prevention of oxidative stress-induced damage in renovascular hypertension.