WoS İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.14627/6
Browse
13 results
Search Results
Article Myrtus Communis Ameliorates Ionizing Radiation-Induced Cardiopulmonary Injury in Rats: Trod-Grog Study(Keai Publishing Ltd, 2026) Aytekin, Aynur; Isci, Oguzhan; Ozyilmaz, Nagehan; Karaoglu, Sumeyye Yilmaz; Ertas, Busra; Sen, Ali; Atasoy, Beste Melek; Yılmaz Karaoğlu, SümeyyeObjectives: Ionizing radiation (IR), widely used in diagnostic and therapeutic procedures, can damage vital organs such as the heart and lungs through oxidative stress. This study aims to assess the potential radioprotective effect of Myrtus communis (MC) against cardiopulmonary injury. Methods: Thirty female rats were divided into four groups. Control (C) and IR (R) groups received oral saline. The treatment (R+MC) and pretreatment (R+preMC) groups received MC (100 mg/kg) for 4 days (starting on the day of IR) and 8 days (starting 4 days before IR), respectively. All IR-exposed groups (R, R+MC, R+preMC) received a single 10 Gy whole-body irradiation. Histopathological changes were assessed by hematoxylin-eosin staining, while oxidative stress was evaluated by measuring malondialdehyde (MDA), glutathione (GSH), myeloperoxidase (MPO), nitric oxide (NO), superoxide dismutase (SOD), glutathione S-transferase (GST), and tissue factor activity (TFa) levels. Protein profiles in tissues were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Results: Histopathologically, MC reduced alveolar and cardiomyocyte damage in both R+MC and R+preMC groups. IR increased all oxidative stress markers and decreased antioxidant parameters in heart and lung tissues (p < 0.05-0.001). Both MC treatment and pretreatment reversed these effects, significantly reducing oxidative/inflammatory markers and restoring antioxidant enzyme activities (p < 0.05-0.001). The R+preMC group demonstrated a stronger protective effect than the R+MC group. Conclusion: Our study shows that MC has a radioprotective effect on the cardiopulmonary system by decreasing oxidative damage. MC appears to be a promising natural compound for advanced radioprotection research, and further molecular and clinical studies could clarify its mechanisms and potential applications<bold>.</bold>Article Gastroprotective Role of Myrtus Communis in a Dual-Condition Model Combining Diabetes and Postmenopausal Rats: Comparable Outcomes to Estrogen Therapy(Cukurova Univ, Fac. Medicine, 2025) Dagsuyu, Eda; Kadioglu-Yaman, Beril; Sen, Ali; Sener, Goksel; Yanardag, RefiyePurpose: Hormonal changes during menopause, especially the decline in estrogen and progesterone levels, can impair gastrointestinal function by slowing digestion. This study aimed to evaluate the protective effects of Myrtus communis subsp. communis (MC) extract on gastric tissue in a postmenopausal diabetic rat model and to compare its efficacy with estrogen (E2) treatment. Materials and Methods: Female Sprague-Dawley rats were divided into six groups: control (C); ovariectomy (OVX); diabetes (D); ovariectomy and diabetes (OVX+D); ovariectomy, diabetes and estrogen (OVX+D+E2); ovariectomy, diabetes and MC (OVX+D+MC). OVX groups had bilateral ovariectomy utilizing the double dorsolateral method. After a seven-day recovery, diabetes was induced using streptozotocin. OVX+D+E2 and OVX+D+MC groups were treated with 10 mu g/kg s.c. E2 and 100 mg/kg p.o. MC extract, respectively, for four weeks. Stomach tissues were analyzed for biochemical parameters. Results: The OVX, D, and OVX+D showed significant reductions in antioxidant enzyme activities and glutathione levels, and increases in oxidative stress markers and lipid peroxidation compared to the C group. E2 and MC treatments effectively mitigated these alterations, with MC showing comparable or superior effects to E2 in many parameters. MC treatment significantly improved blood glucose levels (-60% reduction in the OVX+D), stomach Na+/K+-ATPase activity (-110% increase in the OVX+D), and glycoprotein component levels. Conclusion: MC extract exhibits potent antioxidant and gastroprotective effects in postmenopausal diabetic rats, comparable to E2 treatment. These findings highlight its potential as a natural therapeutic agent for managing menopause-and diabetes-associated gastric complications.Conference Object The Effect of Myrtus Communis Extract on High Fat Diet Induced Liver Damage in Rats(Wiley, 2025) Sucu, Gizem; Kabatas, Gul Sinemcan; Ertas, Busra; Sen, Ali; Sener, Goksel; Ercan, Feriha; Akakin, DilekArticle Citation - WoS: 3Citation - Scopus: 5<i>cotinus Coggygria</I> Scop. Attenuates Acetic Acid-Induced Colitis in Rats by Regulation of Inflammatory Mediators(Springer, 2023) Sen, Ali; Ertas, Busra; Cevik, Ozge; Yildirim, Aybeniz; Kayali, Damla Gokceoglu; Akakin, Dilek; Sener, GokselIn traditional medicine, many medicinal plants are used in the treatment of various diseases caused by inflammation. The objective of the present study is to elucidate for the first time the effects of Cotinus coggygria (CC) ethanol extract (CCE) on colonic structure and inflammation of acetic acid-induced ulcerative colitis in rats. Colonic damage was assessed using disease activity index score, enzyme-linked immunosorbent assay, and hematoxylin-eosin staining. Also, in vitro antioxidant activity of CCE was investigated by ABTS methods. Total phytochemical content of CCE was measured spectroscopically. Acetic acid caused colonic damage according to disease activity index and macroscopic scoring. CCE significantly reversed these damages. While the levels of proinflammatory cytokines TNF-alpha, IL-1beta, IL-6, and TGF-1beta increased in tissue with UC, IL-10 level decreased. CCE increased inflammatory cytokine levels to values close to the sham group. At the same time, while markers indicating disease severity such as VEGF, COX-2, PGE2, and 8-OHdG indicated the disease in the colitis group, these values returned to normal with CCE. Histological research results support biochemical analysis. CCE exhibited significant antioxidant against ABTS radical. Also, CCE was found to have a high content of total polyphenolic compounds. These findings provide evidence that CCE might be benefit as a promising novel therapy in the treatment of UC in humans due to high polyphenol content and justify the use of CC in folkloric medicine for treatment of inflamed diseases.Article Citation - WoS: 1Neuroprotective Effect Of<i> Myrtus</I><i> Communis</I> Against Ionizing Radiation-Induced Brain Injury: Insights From Histopathological and Biochemical Analysis in Rats(Elsevier, 2024) Aslan, Dicle; Alan, Burcu; Yay, Nagehan Ozyilmaz; Karaoglu, Sumeyye Yilmaz; Ertas, Buesrara; Sen, Ali; Atasoy, Beste M.Aim: To investigate the potential radioprotective effects of Myrtus communis on brain tissue. Methods: Thirty female rats were divided into four groups. The control group (C) was applied with oral saline solution (SF) for four days. Myrtus communis (MC) groups started to receive MC (100 mg/kg, oral) either four days before (R + preMC) or immediately after (R + MC) irradiation for four days. Irradiation was applied 10 Gy in a single fraction. All rats were sacrificed on the fourth day of irradiation. Malondialdehyde (MDA), nitric oxide (NO), and glutathione (GSH) levels, myeloperoxidase (MPO), superoxide dismutase (SOD), and tissue factor activities (TFa) were determined for biochemical analysis. Hematoxylin&Eosin &Eosin staining was done for histopathological analyses, and electrophoretic analyses were performed. Results: NO, MDA, and MPO levels were higher in all irradiated groups compared with the C group. MC administration decreased NO, MDA, and MPO levels in R + preMC and R + MC groups. MC administration increased GSH levels. TFa activity decreased in R groups but did not change with MC administration compared to the C group. Radiation-induced brain tissue injury decreased, and morphologically normal neurons were observed in both MC-added groups. Conclusion: Myrtus communis has a potential neuroprotective effect on brain tissue, attributed to its antioxidative, anti-inflammatory, and anti-lipid peroxidative properties.Article Citation - WoS: 1Histological and Biochemical Effects of an Ethanolic Extract of <i>myrtus Communis</I> Leaf on the Pancreases of Rats Fed High Fat Diets(Taylor & Francis Ltd, 2024) Kabatas, Gul Sinemcan; Ertas, Busra; Sen, Ali; Sener, Goksel; Ercan, Feriha; Akakin, DilekWe investigated the effects of an ethanolic extract of Myrtus communis subsp. communis (MC) leaves on the pancreases of rats fed with a high fat diet (HFD). Wistar albino rats were fed either with standard lab chow (Control group) or with a 45% fat diet (HFD and HFD+MC groups) for 4 months, with the MC extract (100 mg/kg) being administered by orogastric gavage to rats in the HFD+MC group during the last month. Blood and pancreas samples were collected from all experimental groups at the end of the study. Insulin and leptin levels, and the lipid profile, were analyzed in the blood serum. Pancreatic injury was assessed histologically. Insulin, nuclear factor kappa beta (NF-kappa B), and alpha-smooth muscle actin (alpha-SMA) were assessed using immunohistochemistry. Apoptosis was assessed using terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) immunohistochemistry. In addition, oxidant/antioxidant activity was analyzed by biochemical methods. Increased body weight, serum insulin and leptin levels, blood glucose level and pancreatic tissue malondialdehyde (MDA), 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels, myeloperoxidase (MPO) activity and decreased tissue glutathione (GSH) level were observed in the HFD group compared to the Control group, in addition to dyslipidemia. An increased histopathological damage score, pancreatic islet area, insulin, TUNEL, NF-kappa B and alpha-SMA immunoreactivity were seen in animals from the HFD group compared to the Control group. However, such pathological changes were reduced in the HFD+MC group. Our data indicate further investigation of MC extract as a therapeutic adjuvant for HFD-induced pancreatic injury, acting via anti-inflammatory and antioxidant mechanisms, is worth carrying out.Article Citation - WoS: 1Ameliorative Effects of Myrtus Communis L. Extract Involving the Inhibition of Oxidative Stress on High Fat Diet-Induced Testis Damage in Rats(Taylor & Francis Ltd, 2024) Coskunlu, Busra; Koroglu, M. Kutay; Hersek, Irem; Ertas, Busra; Sen, Ali; Sener, Goksel; Ercan, FerihaThe possible protective effects of Myrtus communis L. (MC) extract on a high fat diet (HFD)-induced testicular injury in a rat model were investigated using histological and biochemical methods. Wistar albino rats were divided into three groups: a standard diet control group; a HFD group; and an HFD+MC group. The HFD and HFD+MC groups were fed with a HFD for 16 weeks. MC extract (100 mg/kg) was given orally five days a week to the rats in the HFD+MC group during the last four weeks of the experiment. Leptin, triglyceride, high-density lipoproteins, cholesterol, estrogen, testosterone, LH and FSH were analyzed in blood serum. Sperm parameters were evaluated from the epididymis. Testicular morphology, proliferative, apoptotic and NADPH oxidase-2 (NOX2)-positive cells were evaluated histologically. Testicular oxidative stress parameters were analyzed biochemically. In the HFD group, lipid and hormone profiles were changed, abnormal spermatozoa, degenerated seminiferous tubules with apoptotic and NOX2-positive cells were increased in number, and sperm motility and germinal proliferative cells decreased compared to the control group. Moreover, testicular malondialdehyde, 8-hydroxy-2-deoxyguanosine and myeloperoxidase levels increased, whereas glutathione and superoxide dismutase levels decreased in the HFD group compared to the control group. All these histological and biochemical features were ameliorated by MC treatment of HFD-fed rats. In conclusion, HFD caused alterations in sperm parameters and testicular morphology by increasing oxidative damage and apoptosis. MC extract may have potential protective effects by inhibiting oxidative damage.Article Citation - WoS: 4Citation - Scopus: 4Effects of <i>crataegus Orientalis</I> Fruit Extract on Lipid Accumulation in Oleic Acid-Induced Hepg2 Cells(Springer, 2024) Algul, Kader Fatma; Sekerler, Turgut; Sen, Ali; Gulmez, Gizem; Sener, AzizeDuring the recent years, much great deal of research has been conducted on potential use of certain herbs and nutraceuticals to manage nonalcoholic fatty liver disease (NAFLD), specifically with those strong biological activities and antioxidant ability. In our research, the goal is to investigation the protective efficacy of the ethanol extract of Crataegus orientalis fruits (COE) aganist lipid accumulation in oleic acid (OA)-induced hepatocellular carcinoma cells (HepG2) at the molecular level. Firstly, antioxidant activity and lipoxygenase activity of the C. orientalis were determined in vitro. For induction of lipid accumulation in HepG2, we established an in vitro model using 1 mM OA. The C. orientalis was evaluated for their ability to prevent from lipid accumulation and effects on sterol regulatory element binding protein-1c (SREBP-1c), acetyl CoA carboxylase (ACC1) and fatty acid synthase (FAS) mRNA levels involved in lipid metabolism. The C. orientalis exhibited moderate antioxidant activity against ABTS and DPPH radicals and a significant anti-inflammatory effect against the 5-lipoxygenase enzyme. The results demonstrated that the COE (100 mu g/ml) inhibited lipid accumulation (p < 0.01) and was effective in reducing triglyceride (TG) concentration (p < 0.001) and production of reactive oxygen species (ROS) in HepG2 cells (p < 0.05). C. orientalis suppressed mRNA expression of lipid metabolism enzymes (ACC1, FAS) and transcription factor SREBP-1c (p < 0.001, p < 0.001 and p < 0.01, respectively). Our findings recommend that C. orientalis fruits are potentially protective against lipid accumulation in hepatocytes and may have beneficial effects in the treatment of the NAFLD. [GRAPHICS]Article Citation - WoS: 1Morphological Andbiochemical Evaluation of Effects Of<i> Myrtus</I><i> Communis</I> L. Extract on Heart and Aorta in High Fat-Diet Obese Rats(Marmara Univ, Fac Medicine, 2023) Yay, Nagehan Ozyilmaz; Ayci, Nurdan Bulbul; Kaya, Rumeysa Keles; Sen, Ali; Sener, Goksel; Ercan, FerihaObjective: The purpose of this study was to examine the protective effects of Myrtus communis L. (MC) extract on high fat-diet (HFD) induced heart and aorta damage by evaluating oxidative stress and the endothelial nitric oxide system (eNOS).Materials and Methods: Wistar albino male rats were divided into 3 groups (n=7) as control, HFD, and HFD+MC. Rats in HFD and HFD+MC groups were HFD fed for 16 weeks and in the last 4 weeks saline or MC (100 mg/kg) was administered orally (5 days/week). Triglyceride, cholesterol, and high-density lipoprotein (HDL) were estimated in blood serum. Tissue oxidative stress and inflammatory parameters were evaluated biochemically. Tissue morphologies, eNOS, inducible NOS (iNOS), and NADPH oxidase-2 (NOX-2)-immunopositive and apoptotic cells were evaluated histologically.Results: Altered serum lipid profiles, degenerated heart, and aorta morphology, increased malondialdehyde, 8-hydroxy-2-deoxyguanosine, tumor necrosis factor-alpha, monocyte chemoattractant protein-1 and myeloperoxidase levels, and iNOS, NOX-2 immunopositive and apoptotic cells, decreased NO levels, eNOS-immunopositive cells in both tissues were observed in HFD group. All these parameters improved in the HFD+MC group. Conclusion: This study revealed that HFD-induced obesity increased iNOS activation and oxidative stress in the cardiac and aortic tissues of the rats. MC improved oxidant/antioxidant balance and prevented heart and aorta damage via eNOS involvement.Article Citation - WoS: 1Citation - Scopus: 3Investigation of Possible Neuroprotective Effects of Some Plant Extracts on Brain in Bile Duct Ligated Rats(Wiley, 2021) Ozel, Armagan Begum; Cilingir-Kaya, Ozlem Tugce; Sener, Goksel; Ozbeyli, Dilek; Sen, Ali; Sacan, Ozlem; Yarat, AysenThis study aimed to investigate the possible neuroprotective effects of bitter melon (BM), chard, and parsley extracts on oxidative damage that may occur in the brain of rats with bile duct ligation (BDL)-induced biliary cirrhosis. It was observed that lipid peroxidation (LPO), sialic acid (SA), and nitric oxide (NO) levels increased; glutathione (GSH) levels, catalase (CAT) activity, and tissue factor (TF) activity decreased significantly in the BDL group. However, in groups with BDL given BM, chard, and parsley extracts LPO, SA, NO levels decreased; GSH levels and CAT activities increased significantly. No significant differences were observed between groups in total protein, glutathione-S-transferase, superoxide dismutase, and boron. Histological findings were supported by the biochemical results. BM, chard, and parsley extracts were effective in the regression of oxidant damage caused by cirrhosis in the brain tissues. Practical applications Bitter melon (BM), chard, and parsley have antioxidant properties due to their bioactive compounds which are involved in scavenging free radicals, suppressing their production, and stimulating the production of endogenous antioxidant compounds. Since BM, chard, and parsley extracts were found to be effective in the regression of oxidant damage caused by cirrhosis in the brain tissues, these plant extracts may be an alternative in the development of different treatment approaches against brain damage in cirrhosis. At the same time, these species have been used as food by the people for many years. Therefore, they can be used safely as neuroprotective agents in treatment.