WoS İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.14627/6
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Article The Effects of Chard Extract Against Streptozotocin-Induced Erectile Dysfunction in Rats(Istanbul Univ, Fac Pharmacy, 2024) Aydin, Mustafa; Sacan, Ozlem; Kabasakal, Levent; Cetinel, Sule; Kadihasanoglu, Mustafa; Kendirci, Muammer; Sener, Goksel; Sönmez, Yeşim İpçi; Yanardag, RefıyeBackground and Aims: To analyze the potential therapeutic effects of chard against streptozotocin (STZ) -induced erectile dysfunction (ED) and oxidative damage in the corpus cavernousum in rats. Materials and Methods: In this study, Sprague-Dawley rats (250-300g) were allocated into groups as follows: control, diabetic, diabetic + chard, and diabetic + insulin. In order to induce diabetes, rats were given 65 mg/kg intraperitoneal streptozotocin. Chard extract was given orally at a dose 2 g/kg for 45 days beginning on 15 th days. Sixty days after STZ injection, intracavernosal pressure (ICP) was measured and rats were decapitated. Blood samples were obtained for glucose, asymmetric dimethylarginine (ADMA)levels, and lactate dehydrogenase (LDH) activity while cavernous tissues were taken to analyze luminol and lucigenin chemiluminescence (CL), malondialdehyde and glutathione and along with histological analysis. Results: The results revealed that diabetes caused significant decreases in cavernosal tissue glutathione levels, while luminol and lucigenin CL, and malondialdehyde levels were significantly elevated. Plasma glucose, ADMA levels, and LDH activity were also found to be increased in diabetic group. On the other hand, both chard extract and insulin treatment reversed these biochemical parameters significantly. Furthermore, it was found that the ICP value examined for evaluating erectile functions were lower in the diabetic group, but increased in both treatment groups which were similar to the control values. Conclusion: According to our results, chard extract, similar to insulin, reduced diabetes -induced oxidative damage in cavernosal tissue and protected erectile functions. This effects may be attributed its hypoglycemic and antioxidant properties.Article Citation - WoS: 6Citation - Scopus: 6<i>beta Vulgaris</I> L. Var. Cicla Improves Memory Deficits in Intracerebroventricular Streptozotocin Injected Rats: Role on Neuroinflammation(Marmara Univ, 2021) Ertas, Busra; Topal, Fadime; Gulhan, Rezzan; Yanardag, Refiye; Sacan, Ozlem; Sener, Goksel; Aker, Rezzan GulhanAlzheimer's disease is a challenging disease for patients due to progressive loss of cognition and behavioral disorders. Disruption of cholinergic transmission and neuroinflammation are the most important mechanisms underlying cognitive damage. Beta vulgaris L. var. cicla (BV) has been reported to have various pharmacological effects associated with its rich antioxidant content. In addition, anti-cholinesterase and antiinflammatory activities of BV have been demonstrated in vitro. The aim of this study is to elucidate the therapeutic effect of BV against cognitive impairment, reduction in cholinergic transmission and neuroinflammation caused by intracerebroventricular (ICV) administration of streptozotocin (STZ). STZ was administered bilaterally at a dose of 3 mg/kg via ICV to rats, and BV treatment at a dose of 2 g/kg for 21 days was administered orally to STZ-induced animals. After behavioral tests, AChE activity, TNF-alpha and IL-1 beta levels were measured in hippocampus and cortex tissues excised from decapitated animals. Novel object recognition and passive avoidance test showed that the treatment of BV reverted the ICV-STZ induced memory dysfunctions in rats. Furthermore, increased AChE levels in the hippocampal and cortical tissues of STZ-induced rats were significantly reduced with 21 days of BV treatment. In conclusion, these results confirm that STZ administration caused cholinergic hypofunction, neuronal inflammation and cognitive dysfunction in rats, and BV therapy significantly inhibited these changes with its potential neuroprotective activity.Article Citation - WoS: 18<i>petroselinum Crispum</I> Extract Ameliorates Scopolamine-Induced Cognitive Dysfunction: Role on Apoptosis, Inflammation and Oxidative Stress(Tsinghua Univ Press, 2022) Sener, Goksel; Karakadioglu, Gozde; Ozbeyli, Dilek; Ede, Seren; Yanardag, Refiye; Sacan, Ozlem; Aykac, AsliThis study was designed to investigate whether Petroselinum crispum (PC) extract has protective effects on the brain in the scopolamine-induced Alzheimer's disease (AD) rut model. The rats were divided into; control, scopolamine (1 mg/kg, i.p.), galantamine (1.5 mg/kg, i.p.) and PC extract (2 g/kg, p.o.)-treated scopolamine groups. On day 14, the novel object recognition test (NORT) and Morris water maze test (MWMT) were performed and then the rats were sacrificed. Scopolamine-induced cognitive impairments observed in the NORT and MWMT, significantly improved with PC extract and galantamine treatments. Scopolamine reduced M, receptor expression, Bcl-2/Bax ratio, and glutathione levels in the hippocampus and frontal cortex, while malondialdehyde levels, caspase-3/9 expressions, and acetylcholinesterase (AChE) activity were found to be increased. On the other band, PC and galantamine treatments reversed these changes. In conclusion, PC extract has shown an ameliorative effect on the spatial and recognition memory, M-1 receptor expression, apoptosis, oxidative stress, and increased AChE activity. Thus, it was concluded that PC could prevent AD-like conditions and can be used as a functional food. However, since animal models do not completely mimic those of humans, based on the data obtained in this study, the importance of PC on human AD should be demonstrated in future studies. (C) 2022 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.