WoS İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.14627/6
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Article Dodder (Cuscuta sp.) Extract Prevents Cognitive Deficits in a Rat Model of Hepatic Encephalopathy(Kare Publ, 2024) Cevik, Ozge; Sen, Ali; Şener, Göksel; Ercan, Feriha; Doğan, Ahmet; Özbeyli, Dilek; Hatipoğlu, Bilge Nur; Albayrak, Omercan; Koyuncuoglu, TurkanOBJECTIVE: In our study, the protective effect of dodder plant extract against encephalopathy induced by cholestatic liver disease model was investigated. METHODS: Spraque Dawley rats were used in the study. For the cholestatic liver disease model, the bile duct ligation (BDL) was applied. The groups were determined as control, Cuscuta sp. (CUS), BDL and BDL + CUS. Double ligation was performed in the bile duct in the BDL groups. For the applications, saline (SF) was administered to the control and BDL groups for 28 days while 250 mg/ kg of Cuscuta sp. extract was given by oral gavage to the CUS and BDL + CUS groups. At the end of the experiment, cognitive eval- uations were made by applying new object recognition and Morris water maze tests. After these tests, blood-brain barrier (BBB) measurements were made in half of the groups. In the other half of the groups, brain tissue samples were taken by decapitation and transforming growth factor-beta (TGF-β), 8-hydroxydeoxyguanosine (8-OHdG) and sodium-potassium adenosine triphospha- tase (Na+/K+-ATPase) measurements were made in the tissues. Histological examinations of the tissues were also performed. RESULTS: Cognitive performance was low, and BBB permeability was found to be increased in the group with bile duct liga- tion. In addition, TGF-β and 8-OHdG levels were increased in tissues, while Na+/K+-ATPase enzyme activity was suppressed. Treatment with Cuscuta sp. increased cognitive performance and decreased BBB permeability. Other biochemical parameters examined were significantly (p<0.05–0.001) reversed and supported by histological findings. CONCLUSION: Our findings in the study suggest that dodder plant may be beneficial for the protection of cognitive perfor- mance and brain tissue in encephalopathy caused by cholestasis. Keywords: Cuscuta sp.; cholestasis; encephalopathy; fibrosis.Article Citation - WoS: 5Citation - Scopus: 6Hepatoprotective Effects of Parsley (petroselinum Crispum) Extract in Rats With Bile Duct Ligation(Elsevier, 2023) Ede, Seren; Özbeyli, Dilek; Erdogn, Omer; Cevik, Ozge; Kanpalta, Fatma; Ercan, Feriha; Senerg, Goksel; Şener, Göksel; Erdoğan, ÖmerBackground and study aims: This study aimed to investigate the possible protective effects of parsley extract (Petroselinum Crispum; PC) against oxidative liver damage caused by bile obstruction in rats. Material and methods: Bile duct ligation (BDL) method was used to induce liver injury in rats. The rats were divided into the three groups each consisting of 8 rats; Sham-operated control (C), bile duct ligated + saline treated (BDL), and BDL + PC treated groups. PC extract was given at a dose of 2 g/kg orally for 28 days. Aspartate amino transferase (AST), alanin amino transferase (ALT), and bilirubin levels were analyzed in sera. In order to determine free radicals in liver injury, luminol and lucigenin chemiluminescence tests used. Oxidative stress was evaluated through superoxide dismutase, glutathione, malondialdehyde, Na+/K+-ATPase and 8-hydroxy guanosine levels. Furthermore, inflammation marker myeloperoxidase, apoptosis marker caspase-3, and fibrosis markers TGF- beta and hydoxyproline were investigated. The liver tissues were also examined for histological evaluations.Results: While PC treatment decreased AST and ALT levels which increased with BDL, oxidant damage parameters also decreased with this treatment. Conclusion: The present study, which is the first research for PC extract on cholestasis induced liver damage, demonstrated that PC extract could be a potential therapeutic agent against liver fibrosis and need further studies.