WoS İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.14627/6
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Article Citation - WoS: 1Citation - Scopus: 1Synthesis, Characterization, and Antimicrobial Evaluation of Some Novel Hydrazinecarbothioamides(Galenos Publishing House, 2025) Güler, Emrah; Dinç, Harika Öykü; Dincel, Efe Doğukan; Güzeldemirci, Nuray Ulusoy; Başoğlu-ünal, Faika; Kuran, Ebru Dıdem; Başoğlu, Faika; Ulusoy Güzeldemirci, NurayObjectives: This study focused on synthesizing and characterizing novel thiosemicarbazide derivatives containing a 1,2,4-triazole moiety and evaluating their antimicrobial activity against several bacterial strains. The research aimed to identify key structural features that enhance antimicrobial efficacy through structure-activity relationship analysis and identify the minimum inhibitory concentration (MIC) of the most potent compounds to assess their potential for further development as antimicrobial agents. Materials and Methods: Nine novel thiosemicarbazide derivatives containing a 1,2,4-triazole moiety were synthesized by reacting 1,2,4-triazole derivatives with thiosemicarbazide precursors, and the products were characterized using infrared spectroscopy, proton nuclear magnetic resonance (1H-NMR), carbon-13 nuclear magnetic resonance (13C-NMR) spectroscopy, and elemental analysis. The antimicrobial activity of these compounds (5a-i) was tested against Klebsiella pneumoniae (K. pneumoniae), Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, and Pseudomonas aeruginosa (P. aeruginosa), using microdilution, disk diffusion, and broth microdilution methods. Dimethyl sulfoxide was used as a negative control, and Vancomycin and Meropenem were used as positive controls, with all results converted to µM for consistent analysis. Results: The synthesized thiosemicarbazide derivatives (5a-i) were confirmed to be structurally correct through Fourier-transform infrared spectroscopy, 1H-NMR, and 13C-NMR spectroscopy. Among the tested compounds, 5e (4-bromophenyl) and 5g (n-propyl) showed significant antimicrobial activity, with 5g exhibiting the strongest effects against S. aureus and P. aeruginosa. Other derivatives, such as 5b (4-NO2Ph), 5c (4-FPh), and 5d (4-ClPh), showed moderate activity, while no significant activity was observed against K. pneumoniae or E. faecalis. Conclusion: The study successfully synthesized a series of novel thiosemicarbazide derivatives with a 1,2,4-triazole moiety and evaluated their antimicrobial potential. Compounds 5e and 5g exhibited significant antibacterial activity, particularly against S. aureus and P. aeruginosa, with MIC values in the low micromolar range. These findings suggest that the compounds hold promise as potential antimicrobial agents, and further studies should focus on optimizing their efficacy and exploring their mechanism of action.Article Citation - WoS: 3Citation - Scopus: 3Antioxidants, Enzyme Inhibitory Activities, and Phytochemical Profiles of Seven Medicinal Plants Grown With Organic Farming Techniques(Tubitak Scientific & Technological Research Council Turkey, 2023) Zor, Murat; Ozupek, Burcin; Pekacar, Sultan; Deliorman Orhan, Didem; Orhan, Didem DeliormanIn this study, the potential antihyperlipidemic, antidiabetic, and antiobesity effects of extracts obtained via infusion techniques from Artemisia absinthium L., Achillea millefolium L., Calendula officinalis L., Fumaria officinalis L., Mentha piperita L., Origanum onites L., and Urtica dioica L. grown with organic farming techniques were investigated using enzyme inhibition methods (alpha-glucosidase, pan-creatic cholesterol esterase, pancreatic lipase, and alpha-amylase). It is important that natural compounds or extracts to be used in the treat-ment of metabolic problems have antioxidant effects. The antioxidant effects of these species were tested in terms of ABTS and DPPH radical scavenging activity, ferric reducing power, and metal chelating capacity. Extracts (2 mg/mL) of M. piperita (91.43 +/- 0.90%) and O. onites (70.18 +/- 2.02%) showed potent inhibitory effects on the alpha-glucosidase enzyme. Among all species, only O. onites extract ex-erted an inhibitory effect on the cholesterol esterase enzyme close to that of reference compound simvastatin. While all extracts were effective in the tested antioxidant activity methods, it was determined that the M. piperita and O. onites extracts displayed particularly significantly strong activities in terms of ferric reducing power, ABTS radical scavenging effect, and metal chelating capacity. The total flavonoid and phenolic contents of all extracts were determined. The compositions of the most active extracts were analyzed for pheno-lic acids and flavonoids using RP-HPLC. The results of RP-HPLC analysis showed that the levels of ellagic acid (0.923 +/- 0.000 g/100 g extract) in the M. piperita extract and rosmarinic acid (0.813 +/- 0.003 g/100 g extract) in the O. onites extract were high. As a result, all species grown with organic farming techniques except U. dioica were found to have high antioxidant effects. In light of these findings, it was concluded that M. piperita and O. onites grown with organic farming techniques showed strong inhibitory effects in terms of the tested activities, especially for alpha-glucosidase and pancreatic cholesterol esterase, and that in vivo studies and activity-guided isolation studies should be conducted on these species in the future.Article Citation - WoS: 2Citation - Scopus: 2Rapid Synthesis of Silver Nanoparticles With<i> Rheum</I><i> Ribes</I> L Fruit Peels: Anticancer and Antimicrobial Effects With Biocompatible Structures(Ankara Univ, Fac Agriculture, 2024) Zor, Murat; Baran, Mehmet Firat; Ipek, Duygu Neval S. A. Y. I. N.; Sayin İpek, Duygu NevalSilver nanoparticles (AgNPs) are substances with a wide range of uses. Utilizing extracts obtained from the peels of Rheum ribes L. (Rr) fruit growing in Erzurum region, silver nanoparticles were rapidly created in this study with a quick, easy, and environmentally friendly technique without harmful processes. In order to evaluate the attributes of the synthesized Rr-AgNPs, FE-SEM or TEM micrographs were utilized to characterize their morphology. A UV -visible spectrophotometer was used to assess the highest absorbance bands of RR-AgNPs. These data were used to define RR-AgNPs, which were characterized as having exclusively negative surface charges of -25 mV, spherical shape, maximum absorbance at 428 nm wavelength, and 96 nm size distribution. The effectiveness of the produced AgNPs for use in medical applications was assessed using the MTT technique with microdilution. Minimum inhibition concentrations of Rr-AgNPs for pathogen strains ranged from 0.03 to 0.50 mg/L. Additionally, it was discovered that AgNPs effectively suppressed malignant cells, with rates of 86.27%, 74.67%, and 73.49%, in the investigation of the anticancer effects of AgNPs. Healthy cells were not subject to any inhibitory effects at the same concentrations.Article Citation - WoS: 14Citation - Scopus: 12Synthesis of Some Novel Hydrazide-Hydrazones Derived From Etodolac as Potential Anti-Prostate Cancer Agents(Marmara Univ, 2022) Koc, Hande Cevher; Atlihan, Irem; Mega-Tiber, Pinar; Orun, Oya; Kucukguzel, S. Guniz; Tiber, Pinar Mega(R,S)-Etodolac [1,8-diethyl-1,3,4,9-tetrahydrapyrano(3,4-b)indole-1-acetic acid] is a nonsteroidal anti-inflammatory drug that contains carboxylic acid group with the structure of pyrano[3,4-h]indole. In this study, a series of novel (R,S)-Etodolac derivatives (3a-1) bearing hydrazide-hydrazone moiety were synthesized. The structures of these compounds were characterized by spectral (H-1-NMR and FT-IR analyses) methods. All synthesized compounds were screened for anticancer activity against androgen-independent prostate adenocarcinoma (PC-3, DU-145) and androgen-dependent prostate adenocarcinoma (LNCaP) cell lines by using WST-8 colorimetric method. This method was used for cell viability and cytotoxicity analysis. Compound 3b (SGK-720) [2-(1,8-diethyl-1,3,4,9-tetrahydropyrano[3,4-b]indole-1-yl)acetic acid[(2,6-dichlorophenyl)methylene]hydrazides] showed 10.36, 5.24, 15.53 mu M anticancer activity against PC3, DU145, LNCaP cancer cell lines, respectively. According to JC-1 mitochondrial membrane potential test and Annexin V/PI staining, 3b was found to have apoptotic effect on these cancer cells. It is concluded that compound 3b containing 2,6-dichloro substituents may be one of the candidate molecules to cope with prostate cancer.Article Brain in Metabolic Syndrome Model: the Effect of Exercises and Caloric Restriction(Marmara Univ, 2022) Alev-Tuzuner, Burcin; Genc-Kahraman, Nevin; Ipekci, Hazal; Ustundag, Unsal Veli; Tunali-Akbay, Tugba; Emekli-Alturfan, Ebru; Yarat, Aysen; Alturfan, Ebru Emeklı; Akbay, Tugba TunalıCaloric restriction (CR) and exercise (EX) have impacts on improving metabolic risk factors. This study aimed to investigate the changes in the brain after EX and/or CR in metabolic syndrome (MeS) induced by a high fructose diet in rats. Sprague-Dawley male rats were divided into five groups. Drinking water including 10% fructose solution was given to rats for 12 weeks to develop a MeS rat model. Animals with MeS were submitted to EX and/or CR for 6 weeks. Blood glucose, and brain tissue damage and antioxidant parameters were measured. Brain lipid peroxidation, sialic acid, mucin, fucose levels increased in the MeS group compared to the control (C) group. These parameters reduced significantly in the metabolic syndrome with caloric restriction (MeS+CR) group, and more significantly in the metabolic syndrome with exercise and caloric restriction group (MeS+EXCR), compared to the MeS group. Glutathione levels, superoxide dismutase and catalase activities decreased in the MeS group compared to the C group, increased both in the MeS+CR group, and MeS+EXCR group compared to the MeS group. High fructose diet consumption can lead to brain tissue damage and decreased antioxidant levels were found to be improved best in the MeS+EXCR group.