WoS İndeksli Yayınlar Koleksiyonu

Permanent URI for this collectionhttps://hdl.handle.net/20.500.14627/6

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Access Right: Closed AccessPublisher: search.filters.publisher.Marmara Univ, Fac Medicine

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  • Article
    Evaluation of the Neuroprotective Effects of Korean Ginseng Root Extract in an Experimental Model of Multiple Sclerosis
    (Marmara Univ, Fac Medicine, 2025) Donmez, Muhammet Oguzhan; Sener, Goksel; Akbay, Tugba Tunali; Sivas, Guzin Goksun; Akakin, Dilek; Unlu, Hilal; Goren, Mehmet Zafer; Goksun Sivas, Guzin; Tunali Akbay, Tugba
    Objective: Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system characterised by demyelination. The aim of this study was to evaluate the neuroprotective effects of Korean ginseng root extract (KGE) using a cuprizone-induced demyelination model. Materials and Methods: C57BL/6 mice were divided into control, demyelination and remyelination groups and each group was treated with KGE. Demyelination was induced with 0.2% cuprizone in the diet for four weeks. KGE (100 mg/kg) was administered by gavage during or after the cuprizone exposure. Body weight, food and water intake, and motor performance parameters were investigated. In addition, glutathione (GSH), glutathione-S-transferase (GST), superoxide dismutase (SOD) malondialdehyde (MDA), oligodendrocyte transcription factor-2 (OLIG2) and myelin basic protein (MBP) levels were measured in brain samples, while MBP and glial fibrillary acidic protein (GFAP) expression was assessed by immunohistochemistry and myelin status was examined using Luxol Fast Blue staining. Results: Korean ginseng root extract prevented myelin loss, promoted remyelination, and improved motor performance. It reduced oxidative stress by increasing GSH, GST, and SOD levels while decreasing MDA. KGE also suppressed demyelination by reducing astrogliosis and restoring OLIG2 and MBP levels. Conclusion: Korean ginseng root extract exhibits neuroprotective properties during demyelination and promotes remyelination, highlighting its therapeutic potential for MS.
  • Article
    NADPH Oxidase-2 Inhibitor Apocynin Attenuates High-Fat Diet-Induced Kidney and Bladder Injury
    (Marmara Univ, Fac Medicine, 2025) Kanpalta Mustafaoglu, Fatma; Ertas, Busra; Sener, Goksel; Ercan, Feriha
    Objective: This study aimed to evaluate the effects of NADPH oxidase-2 (NOX-2) inhibitor apocynin (APC) on high-fat diet (HFD)induced renal and bladder injury. Materials and Methods: Wistar albino rats were divided into 4 groups: Control, HFD, HFD+dimetyl sulfoxide (DMSO), and HFD+APC. Rats in HFD, HFD+DMSO, and HFD+APC groups were fed with HFD for sixteen weeks. In the last 4 weeks of the experiment, either DMSO or APC (25 mg/kg, dissolved in DMSO) was applied to the HFD+DMSO or HFD+APC groups. Lipid profiles and leptin values were measured in blood serum. Renal and bladder oxidant/antioxidant parameters, histological changes in the tissues, NOX-2-, nuclear factor kappa B (NF-& kgreen;B)-immunopositive and apoptotic cells were evaluated. Results: At the end of the experiment, leptin, cholesterol, and triglyceride levels were higher and high-density lipoprotein levels were lower in the HFD and HFD+DMSO groups compared to controls. In these experimental groups, an increase in malondialdehyde, 8-hydroxy-deoxyguanosine and myeloperoxidase levels and a decrease in glutathione levels, as well as an increase in collagen, NOX-2-and NF & kgreen;-B-immunopositive and apoptotic cells were found. Also, a deterioration in kidney and bladder morphology was observed. All these biochemical and histopathological findings improved in the HFD+APC group. Conclusion: High-fed diet causes renal and bladder injury by increasing NOX-2 activity and inflammation via oxidative stress. APC might alleviate tissue injury by inhibiting oxidative stress.