WoS İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.14627/6
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Article In Vivo and In Silico Evaluation of the Effects of Parsley (Petroselinum Crispum L.) Extract on Small Intestinal Tissue in Scopolamine-Induced Cognitive Dysfunction Model(Wiley-VCH Verlag GmbH, 2025) Ertik, Onur; Sacan, Ozlem; Sener, Goksel; Pazarbasi, Seren Ede; Yanardag, RefiyeThe brain-small intestine connection has become important in neurodegenerative diseases in recent years. In particular, the discovery of the relationship between Alzheimer's disease (AD) and the small intestine and the examination of the effects of AD on this tissue are important in this respect. Our study aimed to understand the effects of the experimentally created AD model in rats on the small intestinal tissue and the protective effect of the extract prepared from parsley leaves (PE). The experimental animals were divided into four groups in the study; Control, Scopolamine (Scop), Scop + PE and Scop + Galantamine (GAL). Oxidative stress parameters and activities of some important enzymes were examined in small intestinal tissues taken as a result of the experimental protocol. Additionally, in silico studies were carried out for bioactive molecules found in parsley leaves using data obtained from in vivo enzyme activity results. It was found that parameters examined for the damaged group, Scop, were reversed by PE and GAL treatment. As a result of in silico studies, it was determined that oxypeucedanin and phylloquinone had higher binding affinity than rutin for acetylcholinesterase (AChE). It has been observed that oxidative damage in the small intestine due to AD can be treated by the PE.Article Citation - WoS: 2Citation - Scopus: 3Petroselinum Crispum Extract Prevents Scopolamine-Induced Lens Damage in Rats(Wiley-v C H verlag Gmbh, 2023) Ertik, Onur; Pazarbasi, Seren Ede; Sener, Goksel; Sacan, Ozlem; Yanardag, RefiyeAlzheimer's disease (AD) is a neurodegenerative disease that occurs especially in advanced ages. It reduces the quality of life of both the patient and their relatives. In addition to its primary effects, AD causes metabolic defects and tissues are damaged due to these effects. Oxidative stress damages cells by disrupting antioxidant/oxidant balance in many tissues, especially due to AD. In individuals with AD and the elderly, lens tissue is damaged due to oxidative stress and may cause vision loss. Therefore, it is very important to investigate herbal products that both prevent/cure AD and reduce AD-related oxidative stress, as they may have fewer side effects. In this study, the protective effects of parsley (Petroselinum crispum) extract on lens tissues of an experimental AD model induced by scopolamine were examined and evaluated through biochemical parameters. The result of biochemical experiments and principal component analysis, was observed that parsley extract had a therapeutic effect by reducing oxidative stress in lens tissues of experimentally induced AD rats. It can be suggested that the phenolic and flavonoid-rich content of parsley extract may have caused the reduction of oxidative damage in lens tissues and can be used to protect lens tissue against oxidative stress due to AD disease.Article The Effects of Chard Extract Against Streptozotocin-Induced Erectile Dysfunction in Rats(Istanbul Univ, Fac Pharmacy, 2024) Aydin, Mustafa; Sacan, Ozlem; Kabasakal, Levent; Cetinel, Sule; Kadihasanoglu, Mustafa; Kendirci, Muammer; Sener, Goksel; Sönmez, Yeşim İpçi; Yanardag, RefıyeBackground and Aims: To analyze the potential therapeutic effects of chard against streptozotocin (STZ) -induced erectile dysfunction (ED) and oxidative damage in the corpus cavernousum in rats. Materials and Methods: In this study, Sprague-Dawley rats (250-300g) were allocated into groups as follows: control, diabetic, diabetic + chard, and diabetic + insulin. In order to induce diabetes, rats were given 65 mg/kg intraperitoneal streptozotocin. Chard extract was given orally at a dose 2 g/kg for 45 days beginning on 15 th days. Sixty days after STZ injection, intracavernosal pressure (ICP) was measured and rats were decapitated. Blood samples were obtained for glucose, asymmetric dimethylarginine (ADMA)levels, and lactate dehydrogenase (LDH) activity while cavernous tissues were taken to analyze luminol and lucigenin chemiluminescence (CL), malondialdehyde and glutathione and along with histological analysis. Results: The results revealed that diabetes caused significant decreases in cavernosal tissue glutathione levels, while luminol and lucigenin CL, and malondialdehyde levels were significantly elevated. Plasma glucose, ADMA levels, and LDH activity were also found to be increased in diabetic group. On the other hand, both chard extract and insulin treatment reversed these biochemical parameters significantly. Furthermore, it was found that the ICP value examined for evaluating erectile functions were lower in the diabetic group, but increased in both treatment groups which were similar to the control values. Conclusion: According to our results, chard extract, similar to insulin, reduced diabetes -induced oxidative damage in cavernosal tissue and protected erectile functions. This effects may be attributed its hypoglycemic and antioxidant properties.