Scopus İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.14627/7
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Article Comprehensive Proteomic and Metabolomic Analysis of Novel Substituted Fluoroquinolone Derivatives in Escherichia Coli Isolates(John Wiley and Sons Ltd, 2026) Nigiz Ş.; Kulabaş N.; Türe A.; Kablan S.; Koçak E.; Özkul C.; Küçükgüzel İ.; Koçak, Engin; Nigiz, Şeyma; Kablan, Sevilay Erdoğan; Özkul, Ceren; Kulabaş, Necla; Küçükgüzel, İlkay; Türe, AslıAntimicrobial resistance is one of the most important global problems, and new antibiotic requirements have been emerging as a key point in this issue. In the present work, we focused on the efficiency of two novel promising fluoroquinolone derivatives on resistant Escherichia coli isolates at the molecular level. Their mode of action and adaptation process were evaluated by using proteomics and metabolomics analysis. Proteomics analysis showed that two compounds have an effect mainly on the ribosomal process and energy metabolism. Moreover, we observed compounds that affect various important antimicrobial targets, such as ribosomal subunits, phosphotransacetylase, and chaperone proteins. In metabolomics analysis, we found that compounds altered bacterial metabolism directly. Pathway analysis showed that cofactor biosynthesis and energy metabolism were affected mainly by undertreated groups. Our experiments demonstrated that novel fluoroquinolone derivatives have promising results at the molecular level and results will contribute to further studies. © 2026 John Wiley & Sons Ltd.Article Fully Synthetic, Nature-Inspired Exosome-Mimetics for Melanoma Therapy(Elsevier B.V., 2026) Arda Ozturk N.Z.; Majchrzak O.B.; Ulivi G.; Kirmizibayrak P.B.; Borchard G.; Patrulea V.; Ozer O.; Ozturk, Nahide Zeren Arda; Borchard, Gerrit; Ozer, Ozgen; Patrulea, Viorica; Majchrzak, Oliwia Barbara; Arda Ozturk, Nahide Zeren; Kirmizibayrak, Petek Ballar; Ulivi, GianlucaFully synthetic exosome-mimetics (FSEMs) represent a nature-inspired drug delivery system designed to replicate the key physicochemical and biological properties of natural exosomes, while offering the potential to address limitations in scalability and reproducibility associated with natural exosomes. In this study, we prepared FSEMs at the laboratory scale. We loaded them with (–)-epigallocatechin-3-gallate (EGCG) and microRNA-23a (miR-23a), aiming to co-deliver therapeutic small molecules and nucleic acids for the treatment of melanoma. FSEMs were fabricated using three methods: thin-film hydration, ethanol injection, and microfluidics. They were surface-functionalized with either CD9, a tetraspanin involved in membrane fusion, or TSP-1, an adhesion protein promoting cellular interactions. Through physicochemical characterization via dynamic light scattering, we found that FSEMs were ∼ 100 nm in size, of low polydispersity (∼0.2) and showed a negative zeta potential (∼–55 mV). Both EGCG and miR-23a were efficiently encapsulated. SDS-PAGE analysis confirmed successful protein incorporation and correct positioning. In vitro release studies showed minimal premature leakage, supporting their suitability for cellular uptake-mediated delivery. When tested on melanoma cells (MDA-MB-435) and progenitor human dermal fibroblasts (FE002-SK2), FSEMs selectively killed melanoma cells while sparing fibroblasts. Importantly, EGCG within FSEMs was more effective than the free compound. Compared to conventional DOTAP-based liposomes, FSEMs were more selective and induced less off-target cytotoxicity. This study presents a proof-of-concept for fully synthetic, protein-functionalized FSEMs as dual carriers for both chemical and gene-based agents, offering a safer and potentially more effective alternative to traditional cationic liposomes. These results lay the groundwork for future in vivo validation and translational cancer research. © 2026 The Author(s)Article Social Media and Financial Decisions: The Influence of Socio-Demographics and Financial Literacy(John Wiley and Sons Inc, 2026) Altinbas, H.This study investigates the predictors of individuals' reliance on social media for financial decision-making within the context of Türkiye's high-inflation environment and the associated surge in retail investor participation. Data were collected via an online survey utilizing the OECD's financial literacy toolkit. The results indicate that gender, family structure, high-risk asset preferences, and financial literacy predict social media usage for financial information. Specifically, males, individuals who invest in stocks or cryptocurrencies, and those with higher financial literacy demonstrate a greater propensity to access financial information on social media; conversely, households with children exhibit lower reliance on social media information. © 2026 American Association of Family and Consumer Sciences.Article “Our Lives Have Gone” Experiences in and After Kahramanmaraş\Türkiye Earthquake: A Qualitative Research(Istanbul University-Cerrahpasa, Florence Nightingale Faculty of Nursing, 2025) Gümüş, F.; Dikeç, G.AIM: This study aimed to examine the experiences and emotions of individuals who experienced the Kahramanmaraş earthquake. METHODS: This study’s data, which were collected using qualitative research methods, were collected through face-to-face individual interviews between March 20 and March 21, 2023, after the earthquake in Hatay and Diyarbakır. The purposive sample method, one of the purposeful random sampling methods, was used in the research. A total of 35 individuals formed the study’s sample. Colaizzi’s phenomenological interpretation method was used in the analysis of the data. RESULTS: The data obtained from the individual interviews with the participants were gathered under three main themes: earthquake moment, emotions, and after the earthquake. In the study, it was determined that the participants stated that they were terrified by the noise and shaking at the time of the earthquake; they could not go out immediately, it was cold when they went out, and they witnessed that everywhere was dark and destroyed. It was determined that the emotions experienced by the participants were anger, helplessness, guilt, hope, and hopelessness, and they expressed their needs after the earthquake, the difficulties in getting help, and the losses they experienced. CONCLUSION: Remarkably, the study results are often parallel to acute stress, loss, and grief reactions, and the needs of individuals are the basic principles of psychological first aid. © 2025, Istanbul University-Cerrahpasa, Florence Nightingale Faculty of Nursing. All rights reserved.Article Peloid Therapy Versus Hot Pack Treatment in Patients with Chronic Low Back Pain: Effects on Pain, Function, and Quality of Life(Romanian Association of Balneology, 2025) Akkurt, B.; Alptekin, H.K.; Aydin, T.This study aimed to compare the efficacy of peloid and hotpack for chronic low back pain (CLBP) due to lumbar disc herniation (LDH). Sixty individuals aged 18–50 years with CLBP due to LDH were randomized into two groups: peloid therapy (n=30) and hot pack therapy (n=30). Treatments were applied five days a week for 15 sessions, and all partici-pants received 20 minutes of lumbar TENS per session. Evaluations were conducted before and after treatment, including pain (VAS), Back Pain Functional Scale (BPFS), Oswestry Disability Index (ODI), quality of life (SF-36), range of motion, and finger-floor distance (FFD). Peloid group showed significant improvements in BPFS, physical functioning, role limitations due to emotional problems, vitality, emotional well-being, pain, and general health compared to the hot pack group (p<0.05). Both groups showed significant reducti-ons in VAS, ODI and FFD scores (p<0.05), with no significant between-group differences (p>0.05). In conclusion, while both treatments were effective in reducing pain and disabili-ty, peloid therapy provided superior improvements in functional status and quality of life compared to hot pack therapy. © 2025 by the authors.Article Investigation of the Susceptibility Rates to Ceftazidime-Avibactam and Colistin, Clonal Relationships, and Clinical Data of Patients with Carbapenem-Resistant Klebsiella Pneumoniae Isolates Detected in the ICUs of a Hospital in İstanbul(K Faisal Special Hospital Research Centre, 2026) Akkaya, Yuksel; Aydin, Irfan; Harmankaya, Sebile; Karakul, Mehmet; Aydin, Mehtap; Erdin, Begum Nalca; Kilic, Ibrahim Halil; Karakuş, MehmetBACKGROUND: The increase in carbapenem-resistant K. pneumoniae (CR-Kp) in intensive care units (ICUs) causes treatment difficulties and increases risk in mortality. OBJECTIVES: The aim of this study was to investigate the susceptibility rates of CR-Kp isolates obtained from ICUs to ceftazidime-avibactam (CAZ-AVI) and colistin, carbapenem resistance genes, clonal relationships and clinical characteristics of the patients. DESIGN: Retrospective cohort SETTING: Single-center, University of Health Sciences, & Uuml;mraniye Training and Research Hospital MATERIALS AND METHODS: This study was conducted between June 2023 and December 2024. Isolates were identified using VITEK MS v.3.2, and antibiotic susceptibility testing was performed using the VITEK 2 Compact system. CAZ-AVI susceptibility was determined using disk diffusion, and colistin susceptibility was determined using broth microdilution to determine minimum inhibitory concentration (MIC) values. Carbapenem resistance genes were determined using multiplex real-time polymerase chain reaction (RT-PCR) and clonal relationship arbitrarily primed-polymerase chain reaction (AP-PCR). MAIN OUTCOME MEASURES: Resistance genes of CR-Kp isolates, clonal relationships, CAZ-AVI and colistin resistance, and clinical characteristics of patients SAMPLE SIZE: Ninety-seven isolates from 76 patients RESULTS: Among patients with CR-Kp isolates, central venous catheter use was detected in 59 cases (78%), ventilator-associated pneumonia in 44 cases (58%), and bacteremia in 39 cases (51%), respectively. It was determined that 53 of the patients (70%) died. Using the AP-PCR method, 60 different genotypes were identified among 97 isolates, and clustering was determined in 42 of the isolates (46%). It was determined that 36 (37%) of the isolates were resistant to colistin and 42 (45%) were resistant to CAZ-AVI. NDM+OXA-48, OXA-48, KPC, KPC+NDM, and NDM genes were detected in 40 (43%), 32 (35%), 10 (11%), 2 (2%), and 3 (3%) isolates, respectively. It was determined that 30 (75%) of the isolates with NDM+OXA-48 and only 4 (12%) of the isolates with OXA-48 were resistant to CAZ-AVI. CONCLUSION: In addition to OXA-48, an increase in the frequency of CR-Kp isolates containing the NDM, NDM+OXA-48, KPC+NDM, and OXA-48+KPC genes were also detected. It was also determined that resistance to colistin and CAZ-AVI is increasing. The AP-PCR method can also be used to investigate infections. LIMITATIONS: Single center,Pulsed Field Gel Electrophoresis (PFGE) could not be performed together with AP-PCRArticle Multipl Skleroz Öz Yönetim Ölçeğinin Türk Toplumuna Uyarlanması: Geçerlik ve Güvenirlik Çalışması(Dokuz Eylul University, 2025) Tosun, Anil; Eroglu, NermınGiriş: Multipl Skleroz (MS), fiziksel ve psikolojik hasara neden olan ve oldukça değişken prognoza sahip kronik, otoimmün bir hastalıktır. Bireylerin fiziksel olarak bağımlı hale gelmesine neden olan semptom tedavisi dışında öz bakım becerilerinin geliştirilmesi oldukça önemlidir. Amaç: Araştırma Multipl Skleroz Öz Yönetim Ölçeği (MS-ÖYÖ) Türk toplumuna uyarlanması amacıyla metodolojik olarak planlandı ve uygulandı. Yöntemler: Araştırma, Temmuz 2019-Mayıs 2020 tarihleri arasında bir üniversite hastanesinin nöroloji kliniğine başvuran multipl sklerozlu bireylerle gerçekleştirilmiştir. MSSM-R’nin geçerlik ve güvenirliği 169 katılımcı ile test edilmiştir. Yapı geçerliliği doğrulayıcı faktör analizi (AMOS) ile incelenmiş, test–tekrar test güvenilirliği eşleştirilmiş örneklem t-testleri ve Pearson korelasyon analizi ile değerlendirilmiş ve iç tutarlılık Cronbach’s alpha ile belirlenmiştir. Ayrıca madde analizleri de yapılmıştır. Bulgular: MS-ÖYÖ geçerliği dil geçerliği, yapı geçerliği (DFA) ve kapsam geçerliği ile değerlendirilmiştir. Güvenirliğinde iç tutarlılık analizi (Cronbach’s Alpha) .88 oldukça yüksek bulunmuştur, madde toplam korelasyonu incelenmiş ve herhangi bir maddenin ölçekten çıkarılmamasına karar verilmiştir ve zamana karşı değişmezliğin değerlendirilmesi için test tekrar test güvenilirliği için 30 hastaya iki hafta ara ile ölçek tekrar uygulanmıştır. Sonuç: Multipl Sklerozlu bireylerin öz yönetimlerinin değerlendirmesi amacıyla Türk toplumuna uyarlanan MS-ÖYÖ geçerli ve güvenilir bir araçtır.”Türkçeye uyarlanan MSSM-R, geçerli ve güvenilir bir araç olup, multipl sklerozlu bireylerin öz-yönetim müdahalelerini desteklemek için hem klinik uygulamada hem de araştırmalarda kullanılabilir.Other Akupunkturun İnfertilite Tedavisinde Canlı Doğum ve Gebelik Sonuçlarına Etkisi: Sistematik Derleme(Dokuz Eylul University, 2025) Satılmış, İlkay Güngör; Bursa, Ayşe GülGiriş: İnfertilite tedavisi teknolojinin gelişmesiyle önemli sonuçlar elde etmiş olsa da, başarı sonucunu arttırmak adına infertilite tedavisi ile birlikte kullanılan tamamlayıcı/alternatif tedavilere yönelim artmaktadır. Amaç: Bu sistematik derlemede infertilite tedavisinde akupunktur uygulamasının, gebelik ve canlı doğum sonuçları üzerine etkisini inceleyen araştırma sonuçlarını sistematik olarak değerlendirmek amaçlanmıştır. Yöntem: Bu sistematik derleme başlangıç yıl sınırı yapılmadan Ağustos 2023- Ekim 2023 tarihleri arasında Cochrane, Science Direct ve Pubmed veri tabanları tarandı. Yayınlanan 847 çalışmadan 15 çalışma inceleme kapsamına alındı. Bulgular: Sistematik derlemeye dahil edilen 15 çalışmadan; 6 çalışmada akupunkturun canlı doğum sonucunu olumlu etkilediği 8 çalışmada ise etkilemediği belirlenmiştir. 1 çalışmada örneklem grubundaki kadınların canlı gebelik oranı etkilenmese de 35 yaş üstü kadınlarda akupunkturun etkili olduğu sonucu elde edilmiştir. Çalışmalarda akupunkturun farklı zamanlarda, farklı bölgelere uygulanması, kontrol gruplarında hiç akupunktur uygulanmayan veya plasebo akupunktur uygulanan (sahte iğne ile gerçek akupunktur grubu ile aynı bölgeye uygulananlar veya akupunktur bölgesi olmayan yerlere uygulananlar) grupların oluşturulması gibi heterojeniteler bulunmaktadır. Sonuç: 15 çalışmanın incelendiği sistematik derlemede; akupunkturun gebelik ve canlı doğum oranlarını arttırdığını belirten ve etkilemediği sonucuna ulaşan çalışma sayıları birbirine çok yakındır. Akupunktur uygulanmasının infertilite tedavisinde yer alması, tartışmalı bir konu olarak devamlılığını korumakta ve konu ile ilgili kanıt düzeyi yüksek randomize kontrollü çalışmalara ihtiyaç duyulmaktadır.Article The Destruction of a Generation by War: Syrian Families, Children’s Education, and the Risks for Turkey(Dr. Esmeralda F. Sanchez, 2025) Terekli, M.S.; Beşiktaş, M.Y.This study examines the phenomenon of migration, focusing on Syrian families under temporary protection who migrated to Turkey following the mass movement of people from Syria after 2011. The research explores the education of Syrian children and the risks faced by Turkey in this context. It also evaluates the economic, social, and cultural impacts of the Syrian refugee crisis on Turkey, highlighting both its positive and negative aspects, as well as related security concerns. Syrian children require increased access to psychological and psychiatric healthcare services. However, many are forced to work under harsh conditions as cheap labor. The employment rates of these children, categorized by age groups, exhibit similarities to those in Turkey but differ significantly from global trends. Applying these observations to Turkey, this study discusses the stages of educational services provided to Syrian children and suggests practical recommendations to improve their quality. The findings reveal that although Syrian children increasingly benefit from educational services each year, their participation rates remain below Turkey's national average. The study also highlights deficiencies in education, healthcare, employment, and socioeconomic conditions for children under temporary protection. Predictably, migration flows intensify during periods of intense conflict. Data from international organizations such as the United Nations High Commissioner for Refugees (UNHCR) and the International Organization for Migration (IOM) have been utilized in this research. © 2025 Elsevier B.V., All rights reserved.Article Machine Learning Model for Predicting Multidrug Resistance in Clinical Escherichia Coli Isolates: A Retrospective General Surgery Study(Multidisciplinary Digital Publishing Institute (MDPI), 2025) Tolan, H.K.; Aydın, İ.; Tanyildizi-Kökkülünk, H.; Karakuş, M.; Akkaya, Y.; Kaya, O.; Işman, F.K.Background/Objectives: Escherichia coli is one of the leading causes of surgical site infections (SSIs) and poses a growing public health concern due to its increasing antimicrobial resistance. High rates of extended-spectrum beta-lactamase (ESBL) production among E. coli strains complicate treatment outcomes and emphasize the need for effective surveillance and control strategies. Methods: A total of 691 E. coli isolates from general surgery clinics (2020–2025) were identified using MALDI-TOF MS. Antibiotic susceptibility data and patient variables were cleaned, encoded, and used to predict resistance using the Random Forest, CatBoost, and Naive Bayes algorithms. SMOTE addressed class imbalance, and model performance was assessed through various validation methods. Results: Among the three machine learning models tested, Random Forest (RF) showed the best performance in predicting antibiotic resistance of E. coli, achieving median accuracy, precision, recall, and F1-scores of 0.90 and AUC values up to 0.99 for key antibiotics. CatBoost performed similarly but was less stable with imbalanced data, while Naive Bayes showed lower accuracy. Feature importance analysis highlighted strong inter-antibiotic resistance links, especially among β-lactams, and some influence of demographic factors. Conclusions: This study highlights the potential of simple, high-performing models using structured clinical data to predict antimicrobial resistance, especially in resource-limited clinical settings. By incorporating machine learning into antimicrobial resistance (AMR) surveillance systems, our goal is to support the advancement of rapid diagnostics and targeted antimicrobial stewardship approaches, which are essential in addressing the growing challenge of multidrug resistance. © 2025 by the authors.