Scopus İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.14627/7
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Article Citation - Scopus: 1Aqueous Parsley (Petroselinum crispum) Extract Ameliorated Methotrexate-Induced Brain and Small Intestine Damage in Rats(Ankara Univ, 2025) Saçan, Ozlem; Şener, Göksel; Yanardag, Refıye; Tunali-Akbay, Tugba; Sivas, Guzin Goksun; Karaoğlu, Sümeyye Yılmaz; Dursun, Ercan; Akbay, Tugba Tunali; Yilmaz Karaoğlu, Sümeyye; Tufan, Elif; Tunali Akbay, TugbaMethotrexate (MTX) is a widely used antiarthritic and chemotherapeutic agent known to cause damage to various tissues. This study investigated the potential protective effects of parsley extract against MTX-induced brain and intestinal tissue damage. Sprague-Dawley rats were divided into control, control + parsley, MTX, and MTX + parsley. MTX (20 mg/kg, i.p.) was administered to the MTX and MTX + parsley groups. The control + parsley, and MTX + parsley groups were administered 2 g/kg parsley extract by oral gavage for five consecutive days. After the fifth day, brain and small intestinal tissues were taken. Total protein, nitric oxide, lipid peroxidation, glutathione levels, tissue factor, superoxide dismutase, and glutathione S-transferase activities were determined in these tissues. The protein profiles of the tissues were evaluated using SDS polyacrylamide gel electrophoresis. Parsley administration caused a decrease in lipid peroxidation levels in both tissues of the MTX group. On the other hand, glutathione level, glutathione-S-transferase, and superoxide dismutase activities were found to be increased. On the other hand, parsley decreased the nitric oxide level which was increased in the intestinal tissues of the MTX group. There was no significant change in brain nitric oxide level and tissue factor activity between groups. MTX and parsley administration altered protein expression, leading to the appearance or disappearance of specific bands in intestinal and brain tissues. In conclusion, parsley alleviated MTX-induced damage in brain and intestinal tissues by reducing lipid peroxidation and modulating antioxidant defenses.Article Lactobacillus Rhamnosus GG Alleviates Bisphenol-A Induced Oxidative Stress in Serum(Marmara University, Faculty of Pharmacy, 2025) Şener, Göksel; Tunali-Akbay, Tugba; Dorucu, Dogancan; Ede-Pazarbasi, Seren; Dede, Pınar; Ede-Pazarbas, SerenThe objective of this investigation was to identify changes in the serum oxidant-antioxidant balance of rats exposed to bisphenol A (BPA) and to investigate the impact of Lactobacillus rhamnosus GG (LGG) administration on those changes. Twenty-four rats (Wistar Albino, 250-300 grams, male) were divided into control, BPA, and BPA+LGG groups with an equal number of rats. BPA and LGG were applied to the rats in the relevant groups for six weeks, five days each week. Six weeks later, the blood samples were withdrawn and serum samples were prepared. Total oxidant and antioxidant status (TAS), glutathione, and lipid peroxidation determinations were determined in serum samples, and the oxidative stress index was calculated. BPA exposure decreased serum total antioxidant status and increased serum total oxidative status, oxidative stress index, and lipid peroxidation level compared to the control group. LGG administration improved the increased serum oxidative stress caused by BPA. Administration of LGG to BPA-treated rats reversed oxidative stress-induced changes. In conclusion, administration of Lactobacillus rhamnosus GG to rats for 30 consecutive days prevented oxidative stress in serum caused by bisphenol A.Article The Protective Effects of Myrtus Communis Subsp. on Ovariectomized Diabetic Rats’ Renal and Intestinal Tissues: in Vivo and in Silico Approaches(Taylor and Francis Ltd., 2025) Ertik, O.; Kadıoğlu-Yaman, Beril; Şen, Ali; Şener, Göksel; Yanardag, RefiyeIntroduction: Postmenopausal diabetes is a condition that affects millions of women and their quality of life. Also, kidney and small intestine tissues are damaged due to diabetes. The present study aimed to examine the protective effects of an extract prepared from Myrtus communis leaves on kidney and small intestine tissues against experimentally created postmenopausal diabetes. Methods: For this purpose, experimental rats were randomly divided into six groups (Control; ovariectomy:OVX, diabetic:D, ovariectomy + diabetic:OVX + D, ovariectomy + diabetic + oestrogen:OVX + D+E2, ovariectomy + diabetic + MC: OVX + D+MC) and kidney and small intestine tissues were taken after the experimental procedure. Results: Evaluations of biochemical parameters (glutathione and glutathione-related enzymes, antioxidant enzymes, etc.) showed that MC had a protective effect on kidney and small intestine tissues in diabetes and ovariectomy groups. Conclusion: It can be suggested that MC extract has a protective effect on small intestine and kidney tissues in postmenopausal diabetes and may be a good herbal source for this purpose. © 2024 Informa UK Limited, trading as Taylor & Francis Group.Article Citation - WoS: 1Citation - Scopus: 2The Effect of Whey Proteins on the Brain and Small Intestine Nitric Oxide Levels: Protein Profiles in Methotrexate-Induced Oxidative Stress(Istanbul Univ, 2022) Yilmaz, Sumeyye; Tufan, Elif; Sivas, Guzin Goksun; Gokmen, Begum Gurel; Dursun, Ercan; Ozbeyli, Dilek; Tunali-Akbay, Tugba; Şener, Göksel; Karaoğlu, Sümeyye YılmazObjectives: The aim of this study was to determine the effects of whey proteins on methotrexate (MTX)-induced brain and small intestine damage. Materials and Methods: 30 Sprague Dawley rats (200-300 g) were divided into four groups: Control, control + whey, MTX, and MTX+whey. MTX was administered at 20 mg/kg (single dose) intraperitoneally to the MTX group rats, and 2 mg/kg of whey protein were administered by oral gavage for 10 days to the whey groups. Lipid peroxidation, glutathione, and nitric oxide (NO) levels, as well as glutathione-Stransferase and superoxide dismutase activities were measured in the brain and small intestine. SDS-polyacrylamide gel electrophoresis of the brain and intestine tissues were also carried out. Results: While MTX treatment caused oxidative damage in the brain and small intestine, whey protein administration ameliorated MTXinduced oxidative stress. MTX administration did not change the brain's NO level, while an increase in intestinal NO level was detected. Conclusion: MTX induced oxidative stress in the brain and small intestine changed the protein metabolism in these tissues regardless of reduced food intake. Consecutive 10-day administration of whey proteins has shown its therapeutic effect on MTX-induced brain and small intestine oxidative damage.Article Citation - WoS: 5Citation - Scopus: 7Bitter Melon (Momordica charantia) Fruit Extract Ameliorates Methotrexate-Induced Reproductive Toxicity in Male Rats(Marmara University, 2021) Kanpalta, Fatma; Özbeyli, Dilek; Sen, Ali; Cevik, O. Dağdeviren; Şener, Göksel; Ercan, FerihaObjective: Methotrexate (MTX) is a drug commonly used for the treatment of malign neoplastic and inflammatory diseases. Anti-oxidant and anti-inflammatory effects of bitter melon (BM) were reported. The aim of this study was to examine the effects of BM fruit extract on MTX-induced testicular and epididymal damage. Materials and Methods: Sprague Dawley male rats were divided into three groups (n=8) as control, MTX and MTX+BM. A single dose of MTX (20 mg/kg) was injected intraperitoneally to the MTX and MTX+BM groups. BM fruit extract (600 mg/kg) was applied to the MTX+BM group orally for 5 days. Testes were examined for general histopathology, proliferating and apoptotic cells. The epididymis samples were used for the evaluation of sperm morphology. Oxidative and inflammatory markers were analysed biochemically. Results: Increased abnormal spermatozoa, degenerated seminiferous tubules with increased apoptotic cells and decreased proliferative cells were observed in the MTX group. TNF-α, IL-1β, 8-hydroxy-2-deoxyguanosine and caspase-3 levels increased, superoxide dismutase and catalase levels decreased in both testis and epididymis samples. All these histological and biochemical parameters were ameliorated in the MTX+BM group. Conclusion: Methotrexate causes testis damage by decreasing spermatogenic cells and increasing apoptosis through oxidative stress and inflammation. BM extract improves testis and epididymis damage with its possible anti-oxidant and anti-inflammatory effects. © 2025 Elsevier B.V., All rights reserved.