TR-Dizin İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.14627/9
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Article Citation - WoS: 1Citation - Scopus: 1A Comparative Study of Biochemical, Antimicrobial Effects and Phytochemical Composition Analysis of Glycyrrhiza Glabra L. Varieties Root Extracts(Marmara University, 2025) Sen, Ali; Servı, Hüseyın; Barak, Timur Hakan; Tekin, Fethullah; Şener, Azize; Marzi, Mahdi; Gülmez, GizemPlants are the significant global interest as alternative treatment sources with their biologically activecompounds. This study compares the chemical composition and the antioxidant, antidiabetic, and antimicrobialproperties of ethanol extracts of G. glabra L. two different varieties from different regions. The phytochemicalcompositions was determined using GC-MS. Additionaly, total phenolic (TPC), flavonoid (TFC) and triterpene (TTC)contents were determined. Glycyrrhizic acid contents were analysed by HPLC. G. glabra var. glandulifera (GF1) showedthe highest antioxidant activity. All extracts had strong antidiabetic effects, besides GF1 showing the highest effect. TheMIC values was determined against 8 bacterial and 1 yeast strain and values ranged from 2.500 to 0.500; 2.500 to 0.714;2.500 to 0.714 for G. glabra var. glabra (GB), GF1, G. glabra var. glandulifera (GF2) respectively. Phytochemical studies haveshown that TPC was 100.60±5.06, 127.90±0.30, 69.01±0.30 mg GAE /g extract; TFC was 80.07±0.15, 25.35±0.0, 16.58±0.31mg KE/g and TTC was 217.30±6.05,172.40±2.17, 126.30±4.50 mg OE/g extract for GB, GF1, GF2, respectively. GF1 inparticular has the highest glycyrrhizic acid content. This study will contribute to the creation of new treatment strategiesand potential therapeutic agents in addition to the use of G. glabra L. in traditional treatments. Our study is also apreliminary study for future studies.Article Citation - Scopus: 4Dodder (Cuscuta Sp.) Extract Ameliorates Liver Fibrosis in Bile Duct-Ligated Rats(Istanbul University Press, 2022) Albayrak, O.; Ozbeyli, D.; Sen, A.; Cevik, O.; Erdogan, O.; Ercan, F.; Sener, G.; Ede-pazarbasi, SerenObjective: The aim is to examine the possible protective effect of Cuscuta sp. extract against liver damage induced by biliary obstruction in rats. Materials and Methods: To induce biliary obstruction, the bile duct ligation (BDL) method was used. Sprague Dawley rats were allocated to 4 groups: Control (C), Cuscuta (CUS), bile duct ligation (BDL), and bile duct ligation + Cuscuta (BDL+CUS). Control and BDL rats were given physiological saline (SF), while CUS and BDL+CUS groups were administered 250 mg/ kg of Cuscuta extract by oral gavage. At the end of 28th day, the rats were decapitated, serum and tissue samples were collected, and aspartate aminotransferase (AST), alanine transaminase (ALT), and direct and total bilirubin (DB and TB) levels were determined in blood samples. In liver tissues, transforming growth factor-β (TGF-β), 8-hydroxyguanosine (8-OHdG), hydroxyproline, and sodium-potassium ATPase (Na+/K+-ATPase) levels were determined. Results: Serum samples of rats with cholestasis had high ALT, AST, DB, and TB levels, while TGF-β, 8-OHdG, and hydroxyproline concentrations were found to be significantly high in tissues. Hepatic Na+/K+-ATPase levels were decreased through biliary obstruction. Biochemical parameters were drastically reversed by Cuscuta care; also, this was supported histologically. Conclusion: Results showed that Cuscuta extract, through its antioxidant and anti-inflammatory properties, provided protection against oxidative injury by biliary obstruction. Also, these results confirm the traditional use of Cuscuta sp. as hepatoprotective. © Ankara Medical Journal.All rights reserved.Article Citation - Scopus: 6Myrtus Communis L. Extract Ameliorates High Fat Diet Induced Kidney and Bladder Damage by Inhibiting Oxidative Stress and Inflammation(Istanbul University Press, 2022) Mustafaoglu, F.K.; Ertas, B.; Sen, A.; Akakin, D.; Sener, G.; Ercan, F.Objective: Obesity is associated with many diseases, including urinary system disorders such as chronic kidney disease and overactive bladder syndrome. Myrtus communis L. (MC) extract has been reported to have antioxidant and anti-inflammatory effects. The aim of this study was to investigate the protective effects of MC extract on high-fat diet (HFD)-induced kidney and bladder damage. Materials and Methods: Wistar albino male rats were divided into three experimental groups: control, HFD and HFD+MC. Experimental groups were fed a standard diet (control group) or HFD (HFD and HFD+MC groups) for 16 weeks. MC extract (100 mg/kg) was administered to the HFD+MC group orally during the last 4 weeks (5 days/week) of the experiment. High-density lipoprotein, total cholesterol, triglyceride and leptin levels were measured in blood serum. Tissue malondialdehyde (MDA), glutathione (GSH), 8-hydroxy-2'-deoxyguanosine (8-OHdG) and myeloperoxidase (MPO) levels were evaluated biochemically. Kidney and bladder morphology, NADPH oxidase-2 (NOX-2) and nuclear factor-kappa B (NF-ҡB)-positive and apoptotic cells were evaluated histologically. Results: Lipid profiles altered and leptin levels increased in blood serum. MDA, 8-OHdG and MPO levels increased and GSH level decreased in kidney and bladder in the HFD group. Moreover, degenerated kidney and bladder morphology, increased NOX-2 and NF-ҡB-positive and apoptotic cells were observed in this group. All of these biochemical and histological parameters were ameliorated in the HFD+MC group. Conclusion: HFD-induced obesity causes kidney and bladder damage by oxidative and inflammatory processes. MC extract may reduce oxidative stress and inflammation and play a protective role in obesity-related kidney and bladder damage. © 2022 by the Author(s).Article Citation - WoS: 3Citation - Scopus: 2Morphological and Biochemical Investigation of the Protective Effects of Panax Ginseng on Methotrexate-Induced Testicular Damage(Istanbul Univ, 2023) Karakaya-Cimen, Fatma Bedia; Macit, Caglar; Sivas, Guzin Goksun; Akbay, Tugba Tunali; Sener, Goksel; Ercan, Feriha; Cımen, Fatma Bedia KarakayaObjective: Methotrexate (MTX) is a chemotherapeutic agent that causes testicular toxicity used in the cure of various types of cancer. The anti-oxidant and anti-cancer effects of Panax ginseng (PxG) have been reported in both experimental and clinical studies. This study aims to examine the healing effect of PxG on testicular damage induced by MTX. Materials and Methods: Sprague Dawley male rats (8-week-olds) were used in the study. A single dose ofMTXdissolved in saline (20 mg/kg) was given to MTX and MTX+PxG groups by intraperitoneal injection. PxG dissolved in saline (100 mg/kg) was given by orogastric gavage once a day for 5 days to the MTX+PxG group. Saline was given to the control and MTX groups orally during the experiments. After decapitation, the testis sampleswere obtained. Seminiferous tubules and basement membranewere evaluated histopathologically. Seminiferous tubule diameter and germinal epithelium thickness were measured. Furthermore, oxidative stress parameters such as malondialdehyde, glutathione, superoxide dismutase, and glutathione-S-transferase were measured. Results: MTX treatment caused seminiferous tubule degeneration with a decrease in Johnsen's score, the seminiferous tubule's diameter, and the germinal epithelium's thickness. Parallel with the histopathological results increased testicular oxidative stress with an increase in malondialdehyde level and a decrease of endogenous anti-oxidant activity with a decrease in glutathione level and glutathione-S-transferase and superoxide dismutase activities. PxG treatment improved these histological and biochemical parameters in MTX-induced testis cytotoxicity. Conclusion: MTX treatment causes testicular damage via the oxidative processes. PxG treatment ameliorates MTX-induced testicular damage by inhibiting oxidative stress.Article Citation - Scopus: 1A Systematic Review And Meta-analysis Of The Prevalence, Risk Factors, Agents And Laboratory Diagnosis Of Vulvovaginal Candidiasis İn Türkiye;(Refik Saydam National Public Health Agency (RSNPHA), 2024) Kilbaş, İ.; Kilbaş, E.P.K.; Çİftçİ, İ.H.Objective: Vulvovaginal candidiasis (VVC) is the most common superficial mycosis among women, and it is estimated that 75% of women experience at least one in their lifetime, and about 40-50% have a second exposure. This study aims to determine the prevalence, risk factors, etiological factors and laboratory diagnosis of VVC in Türkiye. Methods: In the study, original scientific articles for the reporting of VVC epidemiological features published in English and Turkish languages in national and international databases (PubMed, Embase, Scopus, Google Scholar, Web of Science and Turkish Medline) between January 1995 and December 2021 were searched. Electronic databases were searched using various combinations of “vaginal yeast infection”, “vaginal candidiasis”, “vulvovaginal candidiasis Turkey”, “candidal vaginitis”, “Candida vaginitis”, “vaginal candidosis”, “Candida species”, “epidemiology” key terms. Results: The mean prevalence of VVC in adult women was found to be 57.91% in all of the 28 included studies, and 68.21% in the 2-18 age group. Considering the causative distribution, the most common Candida albicans (54.76%), Candida glabrata (24.04%), other Candida species (12.29%), Candida krusei (3.68%), Candida kefyr (3.37%), and Candida tropicalis (2.07%) positivity has been reported. In the included studies, the most common predisposing factors of the patients were pregnancy (35.71%) and diabetes (35.71%). When the articles were examined according to the year they were published and the provinces, it was seen that the prevalence of Candida did not differ significantly (p>0.05). In the studies, VITEK®2 (bioMérieux, Marcy l’Etoile, France) automatic identification system (15), Germ tube (7) and CHROMagar (7) diagnostic methods were used to identify strains. As a result of the meta analysis, it was determined that there was a high level of heterogeneity among studies (I2=95.28). Conclusion: As a result of our meta-analysis study, the prevalence of VVC was found to be high in women and children in Turkey. In addition, it was determined that non-albicans species increased in the etiology of VVK. It has been observed that the incidence of C. glabrata species in the etiology of VVC has increased since 1999. Considering that VVK infection increases the susceptibility to other sexually transmitted diseases, as well as the risk of preterm birth and congenital cutaneous candidiasis in pregnant women, it is essential to raise awareness of patients on issues such as treatment follow-up and protection from the disease. © (2024), (Refik Saydam National Public Health Agency (RSNPHA)). All Rights Reserved.Article Citation - Scopus: 2Effects Of Exercise And Calorie Restriction On Brain And Testis İn Natural Aging Model;(Istanbul University Press, 2021) Hakgüder, U.; Üstündağ, Ü.V.; Tüzüner, B.A.; Genç, N.; İpekci, H.; Özçelik, R.; Emekli-Alturfan, E.; Kahraman, Nevin Genc; Alev-tuzuner, Burcin; Şener, Göksel; Yarat, AysenThe aim of our study was to examine the effects of exercise and calorie restriction on various tissue damage and antioxidant parameters in the brain and testis of rats in a natural aging model. For this purpose, male Sprague-Dawley rats were the control group (C), the elderly (A), the elderly with calorie restriction (ACR), the elderly who were exercised (AE) and the elderly who were exercised with calorie restriction (ACRE), they were divided into 5 groups. The control group was composed of three-month-old animals. The other group consisted of 15-month-old rats. Exercise and calorie restriction were applied for 6 weeks. At the end of the experiment, lipid peroxidation (LPO), nitric oxide (NO), glutathione (GSH) levels and superoxide dismutase (SOD), catalase (CAT), glutathione-S-trans-ferase (GST) and tissue factor (TF) were determined in brain and testicular tissues homogenates. As a result of the study, the A group’s brain and testis LPO, NO levels and TF activity increased, GSH levels and SOD, CAT and GST activities decreased, when compared to the C group. As a result of our study, an increase in oxidant damage was observed with TF activity in the brain and testis in the natural aging model, and positive effects of exercise and calorie restriction on the antioxidant levels in the brain were determined, especially in aging. © 2021, Istanbul University Press. All rights reserved.Article Panax Ginseng Extract Ameliorates Methotrexate-Induced Multi-Organ Damage Via the Regulation of Oxidative Stress(Marmara Univ, 2023) Macit, Caglar; Ede-Pazarbasi, Seren; Yilmaz-karaoglu, Suemeyye; Tunali-Akbay, Tugba; Karakaya-Cimen, Fatma Bedia; Ercan, Feriha; Sener, Goksel; Akbay, Tugba Tunalı-Oxidative damage plays an important role in organ toxicities caused by methotrexate (MTX). This study aimed to determine the antioxidant effects of Panax ginseng (PxG) extract against MTX-induced liver, lung, ileum and kidney damage. Twenty-four Sprague Dawley male rats (weight 250-300 g) were used in the study. The animals were randomly separated into three groups: a) Control, b) MTX-treated (MTX) and c) MTX+PxG-treated (MTX+PxG) groups. MTX was administered intraperitoneally at 20 mg/kg, as a single dose to MTX and MTX+PxG groups. PxG was administered orally at 100 mg/kg to the MTX+PxG group for five days. Saline was given to the control and MTX groups for 5 days. At the end of the experiment, liver, lung, ileum, and kidney samples were obtained. Malondialdehyde (MDA) and glutathione (GSH) levels, superoxide dismutase (SOD), glutathione-S-transferase (GST) and tissue factor (TF) activities were determined in all tissues. In addition, histological examinations were done through light microscopy. GraphPad Prism 5v. was used for statistics, and p<0.05 were considered significant. Administration of MTX caused severe injury in tissues. Findings showed that MDA level, SOD, and GST activities were significantly normalized in the MTX+PxG group compared to the control group. A significant reduction in GSH level observed in the MTX group was reversed with PxG administration In addition, TF activity and total protein levels were found to be impaired in the MTX group, but TF activity was significantly improved in liver and lung tissues and total protein level was significantly reversed in lung and ileum in MTX+PxG group. The results of histological examinations showed that MTX-induced damage was ameliorated with the PxG administration. In conclusion, this study shows that Panax ginseng, thanks to its antioxidant properties, reversed MTX-induced tissue damage and therefore may be beneficial against side effects in patients undergoing chemotherapy.Article Citation - WoS: 1Citation - Scopus: 2The Effect of Whey Proteins on the Brain and Small Intestine Nitric Oxide Levels: Protein Profiles in Methotrexate-Induced Oxidative Stress(Istanbul Univ, 2022) Yilmaz, Sumeyye; Tufan, Elif; Sivas, Guzin Goksun; Gokmen, Begum Gurel; Dursun, Ercan; Ozbeyli, Dilek; Tunali-Akbay, Tugba; Şener, Göksel; Karaoğlu, Sümeyye YılmazObjectives: The aim of this study was to determine the effects of whey proteins on methotrexate (MTX)-induced brain and small intestine damage. Materials and Methods: 30 Sprague Dawley rats (200-300 g) were divided into four groups: Control, control + whey, MTX, and MTX+whey. MTX was administered at 20 mg/kg (single dose) intraperitoneally to the MTX group rats, and 2 mg/kg of whey protein were administered by oral gavage for 10 days to the whey groups. Lipid peroxidation, glutathione, and nitric oxide (NO) levels, as well as glutathione-Stransferase and superoxide dismutase activities were measured in the brain and small intestine. SDS-polyacrylamide gel electrophoresis of the brain and intestine tissues were also carried out. Results: While MTX treatment caused oxidative damage in the brain and small intestine, whey protein administration ameliorated MTXinduced oxidative stress. MTX administration did not change the brain's NO level, while an increase in intestinal NO level was detected. Conclusion: MTX induced oxidative stress in the brain and small intestine changed the protein metabolism in these tissues regardless of reduced food intake. Consecutive 10-day administration of whey proteins has shown its therapeutic effect on MTX-induced brain and small intestine oxidative damage.Article Citation - WoS: 6Citation - Scopus: 6<i>beta Vulgaris</I> L. Var. Cicla Improves Memory Deficits in Intracerebroventricular Streptozotocin Injected Rats: Role on Neuroinflammation(Marmara Univ, 2021) Ertas, Busra; Topal, Fadime; Gulhan, Rezzan; Yanardag, Refiye; Sacan, Ozlem; Sener, Goksel; Aker, Rezzan GulhanAlzheimer's disease is a challenging disease for patients due to progressive loss of cognition and behavioral disorders. Disruption of cholinergic transmission and neuroinflammation are the most important mechanisms underlying cognitive damage. Beta vulgaris L. var. cicla (BV) has been reported to have various pharmacological effects associated with its rich antioxidant content. In addition, anti-cholinesterase and antiinflammatory activities of BV have been demonstrated in vitro. The aim of this study is to elucidate the therapeutic effect of BV against cognitive impairment, reduction in cholinergic transmission and neuroinflammation caused by intracerebroventricular (ICV) administration of streptozotocin (STZ). STZ was administered bilaterally at a dose of 3 mg/kg via ICV to rats, and BV treatment at a dose of 2 g/kg for 21 days was administered orally to STZ-induced animals. After behavioral tests, AChE activity, TNF-alpha and IL-1 beta levels were measured in hippocampus and cortex tissues excised from decapitated animals. Novel object recognition and passive avoidance test showed that the treatment of BV reverted the ICV-STZ induced memory dysfunctions in rats. Furthermore, increased AChE levels in the hippocampal and cortical tissues of STZ-induced rats were significantly reduced with 21 days of BV treatment. In conclusion, these results confirm that STZ administration caused cholinergic hypofunction, neuronal inflammation and cognitive dysfunction in rats, and BV therapy significantly inhibited these changes with its potential neuroprotective activity.