TR-Dizin İndeksli Yayınlar Koleksiyonu
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Article Citation - WoS: 1Citation - Scopus: 1A Comparative Study of Biochemical, Antimicrobial Effects and Phytochemical Composition Analysis of Glycyrrhiza Glabra L. Varieties Root Extracts(Marmara University, 2025) Sen, Ali; Servı, Hüseyın; Barak, Timur Hakan; Tekin, Fethullah; Şener, Azize; Marzi, Mahdi; Gülmez, GizemPlants are the significant global interest as alternative treatment sources with their biologically activecompounds. This study compares the chemical composition and the antioxidant, antidiabetic, and antimicrobialproperties of ethanol extracts of G. glabra L. two different varieties from different regions. The phytochemicalcompositions was determined using GC-MS. Additionaly, total phenolic (TPC), flavonoid (TFC) and triterpene (TTC)contents were determined. Glycyrrhizic acid contents were analysed by HPLC. G. glabra var. glandulifera (GF1) showedthe highest antioxidant activity. All extracts had strong antidiabetic effects, besides GF1 showing the highest effect. TheMIC values was determined against 8 bacterial and 1 yeast strain and values ranged from 2.500 to 0.500; 2.500 to 0.714;2.500 to 0.714 for G. glabra var. glabra (GB), GF1, G. glabra var. glandulifera (GF2) respectively. Phytochemical studies haveshown that TPC was 100.60±5.06, 127.90±0.30, 69.01±0.30 mg GAE /g extract; TFC was 80.07±0.15, 25.35±0.0, 16.58±0.31mg KE/g and TTC was 217.30±6.05,172.40±2.17, 126.30±4.50 mg OE/g extract for GB, GF1, GF2, respectively. GF1 inparticular has the highest glycyrrhizic acid content. This study will contribute to the creation of new treatment strategiesand potential therapeutic agents in addition to the use of G. glabra L. in traditional treatments. Our study is also apreliminary study for future studies.Article Citation - Scopus: 4Dodder (Cuscuta Sp.) Extract Ameliorates Liver Fibrosis in Bile Duct-Ligated Rats(Istanbul University Press, 2022) Albayrak, O.; Ozbeyli, D.; Sen, A.; Cevik, O.; Erdogan, O.; Ercan, F.; Sener, G.; Ede-pazarbasi, SerenObjective: The aim is to examine the possible protective effect of Cuscuta sp. extract against liver damage induced by biliary obstruction in rats. Materials and Methods: To induce biliary obstruction, the bile duct ligation (BDL) method was used. Sprague Dawley rats were allocated to 4 groups: Control (C), Cuscuta (CUS), bile duct ligation (BDL), and bile duct ligation + Cuscuta (BDL+CUS). Control and BDL rats were given physiological saline (SF), while CUS and BDL+CUS groups were administered 250 mg/ kg of Cuscuta extract by oral gavage. At the end of 28th day, the rats were decapitated, serum and tissue samples were collected, and aspartate aminotransferase (AST), alanine transaminase (ALT), and direct and total bilirubin (DB and TB) levels were determined in blood samples. In liver tissues, transforming growth factor-β (TGF-β), 8-hydroxyguanosine (8-OHdG), hydroxyproline, and sodium-potassium ATPase (Na+/K+-ATPase) levels were determined. Results: Serum samples of rats with cholestasis had high ALT, AST, DB, and TB levels, while TGF-β, 8-OHdG, and hydroxyproline concentrations were found to be significantly high in tissues. Hepatic Na+/K+-ATPase levels were decreased through biliary obstruction. Biochemical parameters were drastically reversed by Cuscuta care; also, this was supported histologically. Conclusion: Results showed that Cuscuta extract, through its antioxidant and anti-inflammatory properties, provided protection against oxidative injury by biliary obstruction. Also, these results confirm the traditional use of Cuscuta sp. as hepatoprotective. © Ankara Medical Journal.All rights reserved.Article Citation - Scopus: 6Myrtus Communis L. Extract Ameliorates High Fat Diet Induced Kidney and Bladder Damage by Inhibiting Oxidative Stress and Inflammation(Istanbul University Press, 2022) Mustafaoglu, F.K.; Ertas, B.; Sen, A.; Akakin, D.; Sener, G.; Ercan, F.Objective: Obesity is associated with many diseases, including urinary system disorders such as chronic kidney disease and overactive bladder syndrome. Myrtus communis L. (MC) extract has been reported to have antioxidant and anti-inflammatory effects. The aim of this study was to investigate the protective effects of MC extract on high-fat diet (HFD)-induced kidney and bladder damage. Materials and Methods: Wistar albino male rats were divided into three experimental groups: control, HFD and HFD+MC. Experimental groups were fed a standard diet (control group) or HFD (HFD and HFD+MC groups) for 16 weeks. MC extract (100 mg/kg) was administered to the HFD+MC group orally during the last 4 weeks (5 days/week) of the experiment. High-density lipoprotein, total cholesterol, triglyceride and leptin levels were measured in blood serum. Tissue malondialdehyde (MDA), glutathione (GSH), 8-hydroxy-2'-deoxyguanosine (8-OHdG) and myeloperoxidase (MPO) levels were evaluated biochemically. Kidney and bladder morphology, NADPH oxidase-2 (NOX-2) and nuclear factor-kappa B (NF-ҡB)-positive and apoptotic cells were evaluated histologically. Results: Lipid profiles altered and leptin levels increased in blood serum. MDA, 8-OHdG and MPO levels increased and GSH level decreased in kidney and bladder in the HFD group. Moreover, degenerated kidney and bladder morphology, increased NOX-2 and NF-ҡB-positive and apoptotic cells were observed in this group. All of these biochemical and histological parameters were ameliorated in the HFD+MC group. Conclusion: HFD-induced obesity causes kidney and bladder damage by oxidative and inflammatory processes. MC extract may reduce oxidative stress and inflammation and play a protective role in obesity-related kidney and bladder damage. © 2022 by the Author(s).Article Citation - WoS: 3Citation - Scopus: 2Morphological and Biochemical Investigation of the Protective Effects of Panax Ginseng on Methotrexate-Induced Testicular Damage(Istanbul Univ, 2023) Karakaya-Cimen, Fatma Bedia; Macit, Caglar; Sivas, Guzin Goksun; Akbay, Tugba Tunali; Sener, Goksel; Ercan, Feriha; Cımen, Fatma Bedia KarakayaObjective: Methotrexate (MTX) is a chemotherapeutic agent that causes testicular toxicity used in the cure of various types of cancer. The anti-oxidant and anti-cancer effects of Panax ginseng (PxG) have been reported in both experimental and clinical studies. This study aims to examine the healing effect of PxG on testicular damage induced by MTX. Materials and Methods: Sprague Dawley male rats (8-week-olds) were used in the study. A single dose ofMTXdissolved in saline (20 mg/kg) was given to MTX and MTX+PxG groups by intraperitoneal injection. PxG dissolved in saline (100 mg/kg) was given by orogastric gavage once a day for 5 days to the MTX+PxG group. Saline was given to the control and MTX groups orally during the experiments. After decapitation, the testis sampleswere obtained. Seminiferous tubules and basement membranewere evaluated histopathologically. Seminiferous tubule diameter and germinal epithelium thickness were measured. Furthermore, oxidative stress parameters such as malondialdehyde, glutathione, superoxide dismutase, and glutathione-S-transferase were measured. Results: MTX treatment caused seminiferous tubule degeneration with a decrease in Johnsen's score, the seminiferous tubule's diameter, and the germinal epithelium's thickness. Parallel with the histopathological results increased testicular oxidative stress with an increase in malondialdehyde level and a decrease of endogenous anti-oxidant activity with a decrease in glutathione level and glutathione-S-transferase and superoxide dismutase activities. PxG treatment improved these histological and biochemical parameters in MTX-induced testis cytotoxicity. Conclusion: MTX treatment causes testicular damage via the oxidative processes. PxG treatment ameliorates MTX-induced testicular damage by inhibiting oxidative stress.Article Dodder (Cuscuta sp.) Extract Prevents Cognitive Deficits in a Rat Model of Hepatic Encephalopathy(Kare Publ, 2024) Cevik, Ozge; Sen, Ali; Şener, Göksel; Ercan, Feriha; Doğan, Ahmet; Özbeyli, Dilek; Hatipoğlu, Bilge Nur; Albayrak, Omercan; Koyuncuoglu, TurkanOBJECTIVE: In our study, the protective effect of dodder plant extract against encephalopathy induced by cholestatic liver disease model was investigated. METHODS: Spraque Dawley rats were used in the study. For the cholestatic liver disease model, the bile duct ligation (BDL) was applied. The groups were determined as control, Cuscuta sp. (CUS), BDL and BDL + CUS. Double ligation was performed in the bile duct in the BDL groups. For the applications, saline (SF) was administered to the control and BDL groups for 28 days while 250 mg/ kg of Cuscuta sp. extract was given by oral gavage to the CUS and BDL + CUS groups. At the end of the experiment, cognitive eval- uations were made by applying new object recognition and Morris water maze tests. After these tests, blood-brain barrier (BBB) measurements were made in half of the groups. In the other half of the groups, brain tissue samples were taken by decapitation and transforming growth factor-beta (TGF-β), 8-hydroxydeoxyguanosine (8-OHdG) and sodium-potassium adenosine triphospha- tase (Na+/K+-ATPase) measurements were made in the tissues. Histological examinations of the tissues were also performed. RESULTS: Cognitive performance was low, and BBB permeability was found to be increased in the group with bile duct liga- tion. In addition, TGF-β and 8-OHdG levels were increased in tissues, while Na+/K+-ATPase enzyme activity was suppressed. Treatment with Cuscuta sp. increased cognitive performance and decreased BBB permeability. Other biochemical parameters examined were significantly (p<0.05–0.001) reversed and supported by histological findings. CONCLUSION: Our findings in the study suggest that dodder plant may be beneficial for the protection of cognitive perfor- mance and brain tissue in encephalopathy caused by cholestasis. Keywords: Cuscuta sp.; cholestasis; encephalopathy; fibrosis.Article Citation - Scopus: 1A Systematic Review And Meta-analysis Of The Prevalence, Risk Factors, Agents And Laboratory Diagnosis Of Vulvovaginal Candidiasis İn Türkiye;(Refik Saydam National Public Health Agency (RSNPHA), 2024) Kilbaş, İ.; Kilbaş, E.P.K.; Çİftçİ, İ.H.Objective: Vulvovaginal candidiasis (VVC) is the most common superficial mycosis among women, and it is estimated that 75% of women experience at least one in their lifetime, and about 40-50% have a second exposure. This study aims to determine the prevalence, risk factors, etiological factors and laboratory diagnosis of VVC in Türkiye. Methods: In the study, original scientific articles for the reporting of VVC epidemiological features published in English and Turkish languages in national and international databases (PubMed, Embase, Scopus, Google Scholar, Web of Science and Turkish Medline) between January 1995 and December 2021 were searched. Electronic databases were searched using various combinations of “vaginal yeast infection”, “vaginal candidiasis”, “vulvovaginal candidiasis Turkey”, “candidal vaginitis”, “Candida vaginitis”, “vaginal candidosis”, “Candida species”, “epidemiology” key terms. Results: The mean prevalence of VVC in adult women was found to be 57.91% in all of the 28 included studies, and 68.21% in the 2-18 age group. Considering the causative distribution, the most common Candida albicans (54.76%), Candida glabrata (24.04%), other Candida species (12.29%), Candida krusei (3.68%), Candida kefyr (3.37%), and Candida tropicalis (2.07%) positivity has been reported. In the included studies, the most common predisposing factors of the patients were pregnancy (35.71%) and diabetes (35.71%). When the articles were examined according to the year they were published and the provinces, it was seen that the prevalence of Candida did not differ significantly (p>0.05). In the studies, VITEK®2 (bioMérieux, Marcy l’Etoile, France) automatic identification system (15), Germ tube (7) and CHROMagar (7) diagnostic methods were used to identify strains. As a result of the meta analysis, it was determined that there was a high level of heterogeneity among studies (I2=95.28). Conclusion: As a result of our meta-analysis study, the prevalence of VVC was found to be high in women and children in Turkey. In addition, it was determined that non-albicans species increased in the etiology of VVK. It has been observed that the incidence of C. glabrata species in the etiology of VVC has increased since 1999. Considering that VVK infection increases the susceptibility to other sexually transmitted diseases, as well as the risk of preterm birth and congenital cutaneous candidiasis in pregnant women, it is essential to raise awareness of patients on issues such as treatment follow-up and protection from the disease. © (2024), (Refik Saydam National Public Health Agency (RSNPHA)). All Rights Reserved.Article Citation - Scopus: 2Effects Of Exercise And Calorie Restriction On Brain And Testis İn Natural Aging Model;(Istanbul University Press, 2021) Hakgüder, U.; Üstündağ, Ü.V.; Tüzüner, B.A.; Genç, N.; İpekci, H.; Özçelik, R.; Emekli-Alturfan, E.; Kahraman, Nevin Genc; Alev-tuzuner, Burcin; Şener, Göksel; Yarat, AysenThe aim of our study was to examine the effects of exercise and calorie restriction on various tissue damage and antioxidant parameters in the brain and testis of rats in a natural aging model. For this purpose, male Sprague-Dawley rats were the control group (C), the elderly (A), the elderly with calorie restriction (ACR), the elderly who were exercised (AE) and the elderly who were exercised with calorie restriction (ACRE), they were divided into 5 groups. The control group was composed of three-month-old animals. The other group consisted of 15-month-old rats. Exercise and calorie restriction were applied for 6 weeks. At the end of the experiment, lipid peroxidation (LPO), nitric oxide (NO), glutathione (GSH) levels and superoxide dismutase (SOD), catalase (CAT), glutathione-S-trans-ferase (GST) and tissue factor (TF) were determined in brain and testicular tissues homogenates. As a result of the study, the A group’s brain and testis LPO, NO levels and TF activity increased, GSH levels and SOD, CAT and GST activities decreased, when compared to the C group. As a result of our study, an increase in oxidant damage was observed with TF activity in the brain and testis in the natural aging model, and positive effects of exercise and calorie restriction on the antioxidant levels in the brain were determined, especially in aging. © 2021, Istanbul University Press. All rights reserved.Article Citation - WoS: 3Citation - Scopus: 3Antioxidants, Enzyme Inhibitory Activities, and Phytochemical Profiles of Seven Medicinal Plants Grown With Organic Farming Techniques(Tubitak Scientific & Technological Research Council Turkey, 2023) Zor, Murat; Ozupek, Burcin; Pekacar, Sultan; Deliorman Orhan, Didem; Orhan, Didem DeliormanIn this study, the potential antihyperlipidemic, antidiabetic, and antiobesity effects of extracts obtained via infusion techniques from Artemisia absinthium L., Achillea millefolium L., Calendula officinalis L., Fumaria officinalis L., Mentha piperita L., Origanum onites L., and Urtica dioica L. grown with organic farming techniques were investigated using enzyme inhibition methods (alpha-glucosidase, pan-creatic cholesterol esterase, pancreatic lipase, and alpha-amylase). It is important that natural compounds or extracts to be used in the treat-ment of metabolic problems have antioxidant effects. The antioxidant effects of these species were tested in terms of ABTS and DPPH radical scavenging activity, ferric reducing power, and metal chelating capacity. Extracts (2 mg/mL) of M. piperita (91.43 +/- 0.90%) and O. onites (70.18 +/- 2.02%) showed potent inhibitory effects on the alpha-glucosidase enzyme. Among all species, only O. onites extract ex-erted an inhibitory effect on the cholesterol esterase enzyme close to that of reference compound simvastatin. While all extracts were effective in the tested antioxidant activity methods, it was determined that the M. piperita and O. onites extracts displayed particularly significantly strong activities in terms of ferric reducing power, ABTS radical scavenging effect, and metal chelating capacity. The total flavonoid and phenolic contents of all extracts were determined. The compositions of the most active extracts were analyzed for pheno-lic acids and flavonoids using RP-HPLC. The results of RP-HPLC analysis showed that the levels of ellagic acid (0.923 +/- 0.000 g/100 g extract) in the M. piperita extract and rosmarinic acid (0.813 +/- 0.003 g/100 g extract) in the O. onites extract were high. As a result, all species grown with organic farming techniques except U. dioica were found to have high antioxidant effects. In light of these findings, it was concluded that M. piperita and O. onites grown with organic farming techniques showed strong inhibitory effects in terms of the tested activities, especially for alpha-glucosidase and pancreatic cholesterol esterase, and that in vivo studies and activity-guided isolation studies should be conducted on these species in the future.Article Panax Ginseng Extract Ameliorates Methotrexate-Induced Multi-Organ Damage Via the Regulation of Oxidative Stress(Marmara Univ, 2023) Macit, Caglar; Ede-Pazarbasi, Seren; Yilmaz-karaoglu, Suemeyye; Tunali-Akbay, Tugba; Karakaya-Cimen, Fatma Bedia; Ercan, Feriha; Sener, Goksel; Akbay, Tugba Tunalı-Oxidative damage plays an important role in organ toxicities caused by methotrexate (MTX). This study aimed to determine the antioxidant effects of Panax ginseng (PxG) extract against MTX-induced liver, lung, ileum and kidney damage. Twenty-four Sprague Dawley male rats (weight 250-300 g) were used in the study. The animals were randomly separated into three groups: a) Control, b) MTX-treated (MTX) and c) MTX+PxG-treated (MTX+PxG) groups. MTX was administered intraperitoneally at 20 mg/kg, as a single dose to MTX and MTX+PxG groups. PxG was administered orally at 100 mg/kg to the MTX+PxG group for five days. Saline was given to the control and MTX groups for 5 days. At the end of the experiment, liver, lung, ileum, and kidney samples were obtained. Malondialdehyde (MDA) and glutathione (GSH) levels, superoxide dismutase (SOD), glutathione-S-transferase (GST) and tissue factor (TF) activities were determined in all tissues. In addition, histological examinations were done through light microscopy. GraphPad Prism 5v. was used for statistics, and p<0.05 were considered significant. Administration of MTX caused severe injury in tissues. Findings showed that MDA level, SOD, and GST activities were significantly normalized in the MTX+PxG group compared to the control group. A significant reduction in GSH level observed in the MTX group was reversed with PxG administration In addition, TF activity and total protein levels were found to be impaired in the MTX group, but TF activity was significantly improved in liver and lung tissues and total protein level was significantly reversed in lung and ileum in MTX+PxG group. The results of histological examinations showed that MTX-induced damage was ameliorated with the PxG administration. In conclusion, this study shows that Panax ginseng, thanks to its antioxidant properties, reversed MTX-induced tissue damage and therefore may be beneficial against side effects in patients undergoing chemotherapy.Article Citation - WoS: 1Citation - Scopus: 2The Effect of Whey Proteins on the Brain and Small Intestine Nitric Oxide Levels: Protein Profiles in Methotrexate-Induced Oxidative Stress(Istanbul Univ, 2022) Yilmaz, Sumeyye; Tufan, Elif; Sivas, Guzin Goksun; Gokmen, Begum Gurel; Dursun, Ercan; Ozbeyli, Dilek; Tunali-Akbay, Tugba; Şener, Göksel; Karaoğlu, Sümeyye YılmazObjectives: The aim of this study was to determine the effects of whey proteins on methotrexate (MTX)-induced brain and small intestine damage. Materials and Methods: 30 Sprague Dawley rats (200-300 g) were divided into four groups: Control, control + whey, MTX, and MTX+whey. MTX was administered at 20 mg/kg (single dose) intraperitoneally to the MTX group rats, and 2 mg/kg of whey protein were administered by oral gavage for 10 days to the whey groups. Lipid peroxidation, glutathione, and nitric oxide (NO) levels, as well as glutathione-Stransferase and superoxide dismutase activities were measured in the brain and small intestine. SDS-polyacrylamide gel electrophoresis of the brain and intestine tissues were also carried out. Results: While MTX treatment caused oxidative damage in the brain and small intestine, whey protein administration ameliorated MTXinduced oxidative stress. MTX administration did not change the brain's NO level, while an increase in intestinal NO level was detected. Conclusion: MTX induced oxidative stress in the brain and small intestine changed the protein metabolism in these tissues regardless of reduced food intake. Consecutive 10-day administration of whey proteins has shown its therapeutic effect on MTX-induced brain and small intestine oxidative damage.