Küçükgüzel, Şükriye GünizSenkardes, SevilAtlihan, IremCayir, ElifTiber, Pinar MegaOrun, OyaNigiz, SeymaKucukguzel, S. GunizEczacılık Meslek Bilimleri Bölümü2025-01-112025-01-11202301612-18721612-188010.1002/cbdv.2023007662-s2.0-85164983414https://doi.org/10.1002/cbdv.202300766https://hdl.handle.net/20.500.14627/378Mega Tiber, Pinar/0000-0003-0819-0702; NIGIZ, Seyma/0000-0002-8354-5107; Orun, Oya/0000-0003-1581-2207; Gunduz, Miyase Gozde/0000-0002-2287-9509; Ozkul Kocak, Ceren/0000-0002-0921-5863By exploiting the wide biological potential of the hydrazone scaffold, a series of hydrazone derivatives were synthesized, starting from N-(3-hydroxyphenyl)acetamide (metacetamol). The structures of the compounds were determined using IR, H-1 and C-13-NMR, and mass spectroscopic methods. The obtained molecules (3 a-j) were evaluated for their anticancer potential against MDA-MB-231 and MCF-7 breast cancer cell lines. According to the CCK-8 assay, all tested compounds showed moderate to potent anticancer activity. Among them, N-(3-(2-(2-(4-nitrobenzylidene)hydrazinyl)-2-oxoethoxy)phenyl)acetamide (3 e) was found to be the most effective derivative with an IC50 value of 9.89 & mu;M against MDA-MB-231 cell lines. This compound was further tested for its potential effects on the apoptotic pathway. Molecular docking studies was also carried out for 3 e in the colchicine binding pocket of tubulin. Additionally, compound 3 e also demonstrated effective antifungal activity, particularly against Candida krusei (MIC=8 & mu;g/ml), indicating that nitro group at the 4(th) position of the phenyl ring was the most preferable substituent for both cytotoxic and antimicrobial activity. Our preliminary findings suggest that compound 3 e could be exploited as a leading structure for further anticancer and antifungal drug development.eninfo:eu-repo/semantics/closedAccessAntimicrobialCancerHydrazonesMetacetamolMolecular DockingArticleQ3Q4208WOS:00103174080000137417710