Inhibitory Effect of Whey Protein Concentrate on Sars-Cov Furin Activity and Spike Protein-Ace2 Binding in Methotrexate-Induced Lung Damage

dc.authorid Sener, Goksel/0000-0001-7444-6193
dc.authorid Tunali-Akbay, Tugba/0000-0002-2091-9298
dc.authorid DURSUN, Ercan/0000-0001-6025-9565
dc.authorid Tufan, Elif/0000-0003-0684-3693
dc.authorscopusid 57394511800
dc.authorscopusid 57394797300
dc.authorscopusid 57203904440
dc.authorscopusid 57394313900
dc.authorscopusid 57394797400
dc.authorscopusid 6504603410
dc.authorscopusid 55662834600
dc.authorwosid Tunali Akbay, Tugba/AAS-4127-2020
dc.authorwosid Ozbeyli, Dilek/AAC-2498-2020
dc.authorwosid Dursun, Ercan/JXN-9633-2024
dc.contributor.author Yılmaz Karaoğlu, Sümeyye
dc.contributor.author Sivas, Guzin Goksun
dc.contributor.author Gurel-Gokmen, Begum
dc.contributor.author Yilmaz-Karaoglu, Sumeyye
dc.contributor.author Ercan, Dursun
dc.contributor.author Ozbeyli, Dilek
dc.contributor.author Tunali-Akbay, Tugba
dc.contributor.other Eczane Hizmetleri Bölümü
dc.date.accessioned 2025-01-11T13:00:36Z
dc.date.available 2025-01-11T13:00:36Z
dc.date.issued 2022
dc.department Fenerbahçe University en_US
dc.department-temp [Tufan, Elif; Sivas, Guzin Goksun; Gurel-Gokmen, Begum; Yilmaz-Karaoglu, Sumeyye; Ercan, Dursun; Tunali-Akbay, Tugba] Marmara Univ, Dent Fac, Dept Basic Med Sci, Basibuyuk Hlth Campus, TR-34854 Istanbul, Turkey; [Ozbeyli, Dilek] Marmara Univ, Vocat Sch Hlth Serv, Istanbul, Turkey; [Sener, Goksel] Fenerbahce Univ, Vocat Sch Hlth Serv, Istanbul, Turkey en_US
dc.description Sener, Goksel/0000-0001-7444-6193; Tunali-Akbay, Tugba/0000-0002-2091-9298; DURSUN, Ercan/0000-0001-6025-9565; Tufan, Elif/0000-0003-0684-3693 en_US
dc.description.abstract This study aims to investigate the effects of whey proteins on SARS CoV-2 in methotrexate-induced lung tissue damage in rats. To determine the possible effects, rats were divided into four groups as control, control + whey, methotrexate (20 mg/kg, i.p.) and methotrexate + whey. Whey protein concentrate (2 g/kg, oral gavage) was administered for 10 days. Cytokine levels were measured and protein electrophoresis was carried out in serum samples. Lipid peroxidation, nitric oxide and glutathione level, and superoxide dismutase and glutathione S transferase activities were determined in lung samples. Inhibition of SARS CoV-2-targeted lung furin activity and SARS CoV-2 spike protein-angiotensin converting enzyme binding with whey protein concentrate were also measured in each group. In conclusion, whey protein concentrate improved methotrexate-induced lung damage and inhibited lung furin activity targeting SARS-CoV-2 S1/S2 site cleavage and SARS CoV-2 spike protein-angiotensin converting enzyme binding. Whey proteins are potential protective candidates that inhibit SARS CoV-2-related interactions, even in methotrexate-induced lung injury. Practical applications Whey proteins have anticarcinogenic, antihypertensive, antioxidant, antibacterial, antiviral, and immunomodulating properties due to the protein, bioactive peptide, and essential amino acid content. Methotrexate is a folate antagonist and inhibits cell proliferation and purine synthesis. The combined use of whey protein concentrate and methotrexate may be an alternative in the development of new strategies to the treatment approaches against COVID-19. In addition, according to the results of this study, it is thought that the protective effect of whey proteins in healthy conditions before encountering the SARS CoV-2 may be higher than those who have never used it. en_US
dc.description.woscitationindex Science Citation Index Expanded
dc.identifier.citation 5
dc.identifier.doi 10.1111/jfbc.14039
dc.identifier.issn 0145-8884
dc.identifier.issn 1745-4514
dc.identifier.issue 6 en_US
dc.identifier.pmid 34981557
dc.identifier.scopus 2-s2.0-85122143136
dc.identifier.scopusquality Q2
dc.identifier.uri https://doi.org/10.1111/jfbc.14039
dc.identifier.uri https://hdl.handle.net/20.500.14627/41
dc.identifier.volume 46 en_US
dc.identifier.wos WOS:000737574900001
dc.identifier.wosquality Q2
dc.language.iso en en_US
dc.publisher Wiley-hindawi en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.scopus.citedbyCount 4
dc.subject Covid-19 en_US
dc.subject Cytokines en_US
dc.subject Lung en_US
dc.subject Methotrexate en_US
dc.subject Sars Cov-2 en_US
dc.subject Whey Protein Concentrate en_US
dc.title Inhibitory Effect of Whey Protein Concentrate on Sars-Cov Furin Activity and Spike Protein-Ace2 Binding in Methotrexate-Induced Lung Damage en_US
dc.type Article en_US
dc.wos.citedbyCount 5
dspace.entity.type Publication
relation.isAuthorOfPublication 8c521165-5eda-4dde-9444-690cc90fe1f6
relation.isAuthorOfPublication.latestForDiscovery 8c521165-5eda-4dde-9444-690cc90fe1f6
relation.isOrgUnitOfPublication 133d3937-41df-4bab-8a3e-df9596f91b0e
relation.isOrgUnitOfPublication.latestForDiscovery 133d3937-41df-4bab-8a3e-df9596f91b0e

Files