Effect of Flurbiprofen Derivative (sgk597) on Cell Proliferation and Apoptosis of Breast Cancer Cell Lines

dc.contributor.author Atlihan, Irem
dc.contributor.author Sevinc, Sevgi Kocyigit
dc.contributor.author Orun, Oya
dc.contributor.author Yilmaz, Ozgur
dc.contributor.author Kucukguzel, S. Guniz
dc.contributor.author Tiber, Pinar Mega
dc.contributor.other Eczacılık Meslek Bilimleri Bölümü
dc.date.accessioned 2025-01-11T13:00:51Z
dc.date.available 2025-01-11T13:00:51Z
dc.date.issued 2022
dc.description Atlihan, Irem/0000-0003-0351-0279; Yilmaz, Ozgur/0000-0003-3892-2775 en_US
dc.description.abstract Background and Aims: The incidence of breast cancer is increasing day by day, especially in women. The search for new drugs against breast cancer is the focus of attention in research. Breast cancer and prostate cancer have remarkable biological similarities. Therefore, the 4-(4-chlorophenyl)-3-(1-(2-fluoro-[1,1'-biphenyl]-4-yl)ethyl)-5-((4-fluorobenzyl)thio)-4H-1,2,4-triazole (SGK597) compound that is suppressing cell proliferation in prostate cancer, was studied in MCF-7 breast can-cer and MCF-10A mammary epithelial cell lines. Methods: The WST-8 method was used to determine cell viability and cytotoxicity of SGK597 in MCF-7 and MCF10-A cell lines. The JC-1 test was applied to determine changes in mitochondrial membrane potential. The protein expression levels of Bax, Bcl-2, and c-PARP associated with apoptosis were determined using Western blot analysis.Results: After 24 and 48 hours of incubation of SGK597, the IC50 values were 28.74 pM and 17.28 pM for MCF-7; 65.9 pM and 50.5 pM for MCF-10A, respectively. Mitochondrial membrane potential showed a tendency toward depolarization in MCF-7 cells as a result of increasing concentration of SGK597, while the same tendency was not seen for MCF-10A. As a result of western blot experiments, no increase in the Bax/Bcl-2 ratio and c-PARP expression level was observed, indicating no apoptosis.Conclusion: It was observed that the compound SGK597 suppressed MCF-7 cell proliferation. These results indicate that SGK597 may be a candidate compound for use as an anticancer agent. Keywords: Apoptosis, breast cancer, flurbiprofen, thioether, triazole en_US
dc.description.sponsorship TUBITAK [215S009] en_US
dc.description.sponsorship The synthesis of SGK597 was supported by a grant of TUBITAK (Project number: 215S009). Flurbiprofen was supplied by Sanovel Pharmaceutical Industry Inc. en_US
dc.identifier.citation 4
dc.identifier.doi 10.26650/IstanbulJPharm.2022.1094112
dc.identifier.issn 2587-2087
dc.identifier.uri https://doi.org/10.26650/IstanbulJPharm.2022.1094112
dc.identifier.uri https://hdl.handle.net/20.500.14627/77
dc.language.iso en en_US
dc.publisher Istanbul Univ, Fac Pharmacy en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.subject Apoptosis en_US
dc.subject Breast Cancer en_US
dc.subject Flurbiprofen en_US
dc.subject Thioether en_US
dc.subject Triazole en_US
dc.title Effect of Flurbiprofen Derivative (sgk597) on Cell Proliferation and Apoptosis of Breast Cancer Cell Lines en_US
dc.type Article en_US
dspace.entity.type Publication
gdc.author.id Atlihan, Irem/0000-0003-0351-0279
gdc.author.id Yilmaz, Ozgur/0000-0003-3892-2775
gdc.author.institutional Küçükgüzel, Şükriye Güniz
gdc.author.wosid KOCYIGIT SEVINC, SEVGİ/ITT-5404-2023
gdc.author.wosid Atlıhan, İrem/HIZ-7420-2022
gdc.author.wosid Küçükgüzel, Ş.Güniz/AAQ-8954-2021
gdc.author.wosid TIBER, Pinar/ABE-2738-2020
gdc.author.wosid Yilmaz, Ozgur/AAG-2472-2021
gdc.description.department Fenerbahçe University en_US
gdc.description.departmenttemp [Atlihan, Irem] Marmara Univ, Inst Hlth Sci, Dept Biophys, Istanbul, Turkey; [Sevinc, Sevgi Kocyigit] Kutahya Hlth Sci Univ, Fac Med, Dept Biophys, Kutahya, Turkey; [Orun, Oya; Tiber, Pinar Mega] Marmara Univ, Sch Med, Dept Biophys, Istanbul, Turkey; [Yilmaz, Ozgur] TUBITAK Marmara Res Ctr, Mat Inst, Kocaeli, Turkey; [Kucukguzel, S. Guniz] Fenerbahce Univ, Fac Pharm, Istanbul, Turkey en_US
gdc.description.endpage 270 en_US
gdc.description.issue 3 en_US
gdc.description.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
gdc.description.scopusquality N/A
gdc.description.startpage 265 en_US
gdc.description.volume 52 en_US
gdc.description.woscitationindex Emerging Sources Citation Index
gdc.identifier.trdizinid 1168006
gdc.identifier.wos WOS:000954321500003
gdc.wos.citedcount 6
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