Kızıltaş, Gülesme

Profile Picture
Profile URL
https://hdl.handle.net/20.500.14627/686
Name Variants
Yilmaz, Gulesme
Job Title
Öğretim Görevlisi
Email Address
gulesme.yilmaz@fbu.edu.tr
Main Affiliation
Tıbbi Hizmetler ve Teknikler Bölümü
Status
Website
ORCID ID
0000-0002-5789-109X
Scopus Author ID
Turkish CoHE Profile ID
Google Scholar ID
WoS Researcher ID
Files
photo_354404.png.png (193.7 KB)

Sustainable Development Goals

2

ZERO HUNGER
ZERO HUNGER Logo

1

Research Products

8

DECENT WORK AND ECONOMIC GROWTH
DECENT WORK AND ECONOMIC GROWTH Logo

1

Research Products

12

RESPONSIBLE CONSUMPTION AND PRODUCTION
RESPONSIBLE CONSUMPTION AND PRODUCTION Logo

1

Research Products
Scholarly Output

1

Articles

1

Citation Count

1

Supervised Theses

0

Filters

20231
Reset filters

Settings

Scholarly Output Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    Article
    Citation - WoS: 3
    Citation - Scopus: 4
    Comparison of in Vitro Antifungal Activity Methods Using Extract of Chitinase-Producing aeromonas Sp. Bhc02
    (Springer, 2023) Cadirci, Bilge Hilal; Yilmaz, Gulesme; Tıbbi Hizmetler ve Teknikler Bölümü
    Biological control to prevent fungal plant diseases offers an alternative approach to facilitate sustainable agriculture. Since the chitin in fungal cell walls is a target for biocontrol agents, chitinases are one of the important antifungal molecules. In this study, the aim was to investigate a new chitinase isolated from a fluvial soil bacterium and to show the antifungal activity of the characterized chitinase by comparing the three common methods. The bacterium with the highest chitinase activity was identified as Aeromonas sp. by 16 S rRNA sequence analysis. Following the determination of the optimum enzyme production time, the enzyme was partially purified, and the physicochemical parameters of the enzyme were investigated. In the antifungal studies, direct Aeromonas sp. BHC02 cells or partially purified chitinase were used. As a result, in the first method in which the Aeromonas sp. BHC02 cells were spread on the surface of petri dishes, no zone formation was observed around the test fungi spotted on the surface. However, zone formation was observed in the methods in which the antifungal activity was investigated using the partially purified chitinase enzyme. For example, in the second method, the enzyme was spread on the surface of PDA, and zone formation was observed only around Penicillum species among the test fungi spotted on the surface. In the third method, in which the necessary time was given for the formation of mycelium of the test fungi, it was observed that the growth of Fusarium solani, Alternaria alternata and Botrytis cinerea was inhibited by the partially purified chitinase. This study concludes that the results of the antifungal activities depend on the method used and all fungal chitins cannot be degraded with one strain's chitinase. Depending on the variety of chitin, some fungi can be more resistant.