Mehtiyev, Tuğrul

Profile URL
https://hdl.handle.net/20.500.14627/483
Name Variants
Mehtiyev, T.
Job Title
Doktor Öğretim
Email Address
tugrul.mehtiyev@fbu.edu.tr
Main Affiliation
Eczacılık Meslek Bilimleri Bölümü
Status
Website
ORCID ID
0000-0002-8701-7985
Scopus Author ID
Turkish CoHE Profile ID
Google Scholar ID
3caVKjcAAAAJ
WoS Researcher ID
KXR-6930-2024
Files
photo_410974.jpg.JPG (2 MB)

Research Topics

Domains
Physical SciencesLife Sciences
Fields
Environmental ScienceBiochemistry, Genetics and Molecular BiologyAgricultural and Biological Sciences
Subfields
PollutionMolecular BiologyPlant Science
Specific Research Areas
Pesticide and Herbicide Environmental Studies
Epigenetics and DNA Methylation
Weed Control and Herbicide Applications
Pesticide Exposure and Toxicity
Retinoids in leukemia and cellular processes

Sustainable Development Goals

SDG data is not available

Publication Collaboration

Affiliation Name Count
Istanbul University 3
Fenerbahçe University 1
Biruni University 1
1 / 1
Data obtained from OpenAlex
Scholarly Output

1

Articles

1

WoS Citation Count

2

Scopus Citation Count

3

Author of Publication: search.filters.isAuthorOfPublication.2caf25a8-c4f4-4895-8bb6-6f601a353e17

Scholarly Output Search Results

Now showing 1 - 1 of 1
  • Article
    Citation - WoS: 2
    Citation - Scopus: 3
    Glyphosate and AMPA-Induced Apoptosis and Epigenetic Alterations in HepG2 Cells: Upregulation of P53-BAX-CASP Pathways
    (Pergamon-Elsevier Science Ltd, 2025) Mehtiyev, Tugrul; Guler, Zeynep Rana; Aktan, Elif; Ozden, Sibel
    Glyphosate and its metabolite aminomethylphosphonic acid (AMPA) are environmental contaminants with potential toxic effects. This study aimed to investigate apoptosis and epigenetic alterations induced by glyphosate and AMPA in HepG2 cells. The IC50 values for glyphosate and AMPA were 6.19 mM and 8.13 mM, respectively, following 24 h exposure; mechanistic assays were conducted at sub-cytotoxic concentrations (50-500 mu M). Annexin V/PI flow cytometry revealed that AMPA significantly increased early apoptosis (up to 116 %, p < 0.001), while glyphosate elevated late apoptosis (up to 145 %, p < 0.001). Gene expression analysis showed significant upregulation of p53 (>= 1.49-fold), BAX (>= 1.82-fold), CASP3 (>= 1.37-fold), and CASP9 (>= 1.83-fold), with no significant change in BCL2. Epigenetic analysis indicated that both glyphosate and AMPA increased global DNA methylation, with fold changes ranging from 1.43 to 1.62-fold at concentrations of 100-250 mu M (p < 0.05). DNA methyltransferase genes DNMT1 (>= 2.44-fold), DNMT3A (>= 1.65-fold), and DNMT3B (>= 1.65-fold) were upregulated. Additionally, histone modification profiling showed elevated levels of H3K27me3, H3K9me3, and H3K9ac (p < 0.05), alongside increased expression of G9a (>= 1.64-fold), EZH2 (>= 2.14-fold), SETD1B (>= 2.15-fold), HAT1 (>= 2.40-fold), and SIRT1 (>= 2.57-fold), and downregulation of SUV39H1 (>= 0.27-fold). These findings reveal the molecular mechanisms of glyphosate and AMPA toxicity, linking apoptosis to epigenetic alterations and enhancing understanding of their risks.