Browsing by Author "Yanardag, Refiye"

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Citation - WoS: 6
Citation - Scopus: 6
beta Vulgaris L. Var. Cicla Improves Memory Deficits in Intracerebroventricular Streptozotocin Injected Rats: Role on Neuroinflammation
(Marmara Univ, 2021) Ertas, Busra; Topal, Fadime; Gulhan, Rezzan; Yanardag, Refiye; Sacan, Ozlem; Sener, Goksel
Alzheimer's disease is a challenging disease for patients due to progressive loss of cognition and behavioral disorders. Disruption of cholinergic transmission and neuroinflammation are the most important mechanisms underlying cognitive damage. Beta vulgaris L. var. cicla (BV) has been reported to have various pharmacological effects associated with its rich antioxidant content. In addition, anti-cholinesterase and antiinflammatory activities of BV have been demonstrated in vitro. The aim of this study is to elucidate the therapeutic effect of BV against cognitive impairment, reduction in cholinergic transmission and neuroinflammation caused by intracerebroventricular (ICV) administration of streptozotocin (STZ). STZ was administered bilaterally at a dose of 3 mg/kg via ICV to rats, and BV treatment at a dose of 2 g/kg for 21 days was administered orally to STZ-induced animals. After behavioral tests, AChE activity, TNF-alpha and IL-1 beta levels were measured in hippocampus and cortex tissues excised from decapitated animals. Novel object recognition and passive avoidance test showed that the treatment of BV reverted the ICV-STZ induced memory dysfunctions in rats. Furthermore, increased AChE levels in the hippocampal and cortical tissues of STZ-induced rats were significantly reduced with 21 days of BV treatment. In conclusion, these results confirm that STZ administration caused cholinergic hypofunction, neuronal inflammation and cognitive dysfunction in rats, and BV therapy significantly inhibited these changes with its potential neuroprotective activity.
Citation - WoS: 18
petroselinum Crispum Extract Ameliorates Scopolamine-Induced Cognitive Dysfunction: Role on Apoptosis, Inflammation and Oxidative Stress
(Tsinghua Univ Press, 2022) Sener, Goksel; Karakadioglu, Gozde; Ozbeyli, Dilek; Ede, Seren; Yanardag, Refiye; Sacan, Ozlem; Aykac, Asli
This study was designed to investigate whether Petroselinum crispum (PC) extract has protective effects on the brain in the scopolamine-induced Alzheimer's disease (AD) rut model. The rats were divided into; control, scopolamine (1 mg/kg, i.p.), galantamine (1.5 mg/kg, i.p.) and PC extract (2 g/kg, p.o.)-treated scopolamine groups. On day 14, the novel object recognition test (NORT) and Morris water maze test (MWMT) were performed and then the rats were sacrificed. Scopolamine-induced cognitive impairments observed in the NORT and MWMT, significantly improved with PC extract and galantamine treatments. Scopolamine reduced M, receptor expression, Bcl-2/Bax ratio, and glutathione levels in the hippocampus and frontal cortex, while malondialdehyde levels, caspase-3/9 expressions, and acetylcholinesterase (AChE) activity were found to be increased. On the other band, PC and galantamine treatments reversed these changes. In conclusion, PC extract has shown an ameliorative effect on the spatial and recognition memory, M-1 receptor expression, apoptosis, oxidative stress, and increased AChE activity. Thus, it was concluded that PC could prevent AD-like conditions and can be used as a functional food. However, since animal models do not completely mimic those of humans, based on the data obtained in this study, the importance of PC on human AD should be demonstrated in future studies. (C) 2022 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.
In Vivo and In Silico Evaluation of the Effects of Parsley (Petroselinum Crispum L.) Extract on Small Intestinal Tissue in Scopolamine-Induced Cognitive Dysfunction Model
(Wiley-VCH Verlag GmbH, 2025) Ertik, Onur; Sacan, Ozlem; Sener, Goksel; Pazarbasi, Seren Ede; Yanardag, Refiye
The brain-small intestine connection has become important in neurodegenerative diseases in recent years. In particular, the discovery of the relationship between Alzheimer's disease (AD) and the small intestine and the examination of the effects of AD on this tissue are important in this respect. Our study aimed to understand the effects of the experimentally created AD model in rats on the small intestinal tissue and the protective effect of the extract prepared from parsley leaves (PE). The experimental animals were divided into four groups in the study; Control, Scopolamine (Scop), Scop + PE and Scop + Galantamine (GAL). Oxidative stress parameters and activities of some important enzymes were examined in small intestinal tissues taken as a result of the experimental protocol. Additionally, in silico studies were carried out for bioactive molecules found in parsley leaves using data obtained from in vivo enzyme activity results. It was found that parameters examined for the damaged group, Scop, were reversed by PE and GAL treatment. As a result of in silico studies, it was determined that oxypeucedanin and phylloquinone had higher binding affinity than rutin for acetylcholinesterase (AChE). It has been observed that oxidative damage in the small intestine due to AD can be treated by the PE.
Citation - WoS: 5
Citation - Scopus: 6
Melatonin Improves Liver and Pancreatic Tissue Injuries in Diabetic Rats: Role on Antioxidant Enzymes
(Springer int Publ Ag, 2023) Ertik, Onur; Bayrak, Bertan Boran; Sener, Goksel; Yanardag, Refiye
PurposeMelatonin (Mel) is an indolamine mainly synthesized by the pineal gland and many other organs. It plays an important role in scavenging free radicals and stimulating antioxidant enzymes. The goal of this study was to investigate the effect of Mel and/or insulin treatment on oxidative liver and pancreas injuries in diabetic rats.MethodsMale Wistar albino rats were assigned into 5 groups. Group I: control animals. Group II: diabetes was induced via a single dose of STZ (60 mg/kg) administered intraperitoneally. Group III: diabetic rats treated with Mel (10 mg/kg/day). Group IV: diabetic rats given insulin (6U/kg) subcutaneously. Group V: diabetic rats that received insulin and Mel at the same dose and time. After 12 weeks of the experiment, the animals were decapitated, liver and pancreas tissues were collected.ResultsThe results indicated that reduced glutathione levels in liver and pancreatic tissue decreased, while protein carbonyl, advanced oxidized protein products and lipid peroxidation levels were elevated in diabetic group. Antioxidant enzyme activities decreased in liver tissues but increased in pancreatic tissues of the diabetic group. Administration of Mel, insulin or Mel + insulin reversed these biochemical changes in the diabetic animals.ConclusionThis work shows that in long-term oxidative stress conditions caused by STZ-induced diabetes, either Mel or Mel + insulin administration may improve the deteriorated oxidant/antioxidant system in both the liver and pancreas tissues. These results suggested that Mel alone or Mel + insulin treatments might have a significant role in protecting against liver and pancreatic damage in STZ diabetic rats via different antioxidant effects.
Citation - WoS: 2
Citation - Scopus: 3
Petroselinum Crispum Extract Prevents Scopolamine-Induced Lens Damage in Rats
(Wiley-v C H verlag Gmbh, 2023) Ertik, Onur; Pazarbasi, Seren Ede; Sener, Goksel; Sacan, Ozlem; Yanardag, Refiye
Alzheimer's disease (AD) is a neurodegenerative disease that occurs especially in advanced ages. It reduces the quality of life of both the patient and their relatives. In addition to its primary effects, AD causes metabolic defects and tissues are damaged due to these effects. Oxidative stress damages cells by disrupting antioxidant/oxidant balance in many tissues, especially due to AD. In individuals with AD and the elderly, lens tissue is damaged due to oxidative stress and may cause vision loss. Therefore, it is very important to investigate herbal products that both prevent/cure AD and reduce AD-related oxidative stress, as they may have fewer side effects. In this study, the protective effects of parsley (Petroselinum crispum) extract on lens tissues of an experimental AD model induced by scopolamine were examined and evaluated through biochemical parameters. The result of biochemical experiments and principal component analysis, was observed that parsley extract had a therapeutic effect by reducing oxidative stress in lens tissues of experimentally induced AD rats. It can be suggested that the phenolic and flavonoid-rich content of parsley extract may have caused the reduction of oxidative damage in lens tissues and can be used to protect lens tissue against oxidative stress due to AD disease.
The Protective Effects of Myrtus Communis Subsp. on Ovariectomized Diabetic Rats’ Renal and Intestinal Tissues: in Vivo and in Silico Approaches
(Taylor and Francis Ltd., 2025) Ertik, O.; Kadıoğlu-Yaman, Beril; Şen, Ali; Şener, Göksel; Yanardag, Refiye
Introduction: Postmenopausal diabetes is a condition that affects millions of women and their quality of life. Also, kidney and small intestine tissues are damaged due to diabetes. The present study aimed to examine the protective effects of an extract prepared from Myrtus communis leaves on kidney and small intestine tissues against experimentally created postmenopausal diabetes. Methods: For this purpose, experimental rats were randomly divided into six groups (Control; ovariectomy:OVX, diabetic:D, ovariectomy + diabetic:OVX + D, ovariectomy + diabetic + oestrogen:OVX + D+E2, ovariectomy + diabetic + MC: OVX + D+MC) and kidney and small intestine tissues were taken after the experimental procedure. Results: Evaluations of biochemical parameters (glutathione and glutathione-related enzymes, antioxidant enzymes, etc.) showed that MC had a protective effect on kidney and small intestine tissues in diabetes and ovariectomy groups. Conclusion: It can be suggested that MC extract has a protective effect on small intestine and kidney tissues in postmenopausal diabetes and may be a good herbal source for this purpose. © 2024 Informa UK Limited, trading as Taylor & Francis Group.