Browsing by Author "Tunali-Akbay, Tugba"

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Citation Count: 0
Brain in Metabolic Syndrome Model: the Effect of Exercises and Caloric Restriction
(Marmara Univ, 2022) Şener, Göksel; Genc-Kahraman, Nevin; Ipekci, Hazal; Ustundag, Unsal Veli; Tunali-Akbay, Tugba; Emekli-Alturfan, Ebru; Yarat, Aysen; Eczacılık Meslek Bilimleri Bölümü
Caloric restriction (CR) and exercise (EX) have impacts on improving metabolic risk factors. This study aimed to investigate the changes in the brain after EX and/or CR in metabolic syndrome (MeS) induced by a high fructose diet in rats. Sprague-Dawley male rats were divided into five groups. Drinking water including 10% fructose solution was given to rats for 12 weeks to develop a MeS rat model. Animals with MeS were submitted to EX and/or CR for 6 weeks. Blood glucose, and brain tissue damage and antioxidant parameters were measured. Brain lipid peroxidation, sialic acid, mucin, fucose levels increased in the MeS group compared to the control (C) group. These parameters reduced significantly in the metabolic syndrome with caloric restriction (MeS+CR) group, and more significantly in the metabolic syndrome with exercise and caloric restriction group (MeS+EXCR), compared to the MeS group. Glutathione levels, superoxide dismutase and catalase activities decreased in the MeS group compared to the C group, increased both in the MeS+CR group, and MeS+EXCR group compared to the MeS group. High fructose diet consumption can lead to brain tissue damage and decreased antioxidant levels were found to be improved best in the MeS+EXCR group.
Citation Count: 1
The Effect of Whey Proteins on the Brain and Small Intestine Nitric Oxide Levels: Protein Profiles in Methotrexate-Induced Oxidative Stress
(Istanbul Univ, 2022) Yılmaz Karaoğlu, Sümeyye; Tufan, Elif; Sivas, Guzin Goksun; Gokmen, Begum Gurel; Dursun, Ercan; Ozbeyli, Dilek; Tunali-Akbay, Tugba; Eczane Hizmetleri Bölümü
Objectives: The aim of this study was to determine the effects of whey proteins on methotrexate (MTX)-induced brain and small intestine damage. Materials and Methods: 30 Sprague Dawley rats (200-300 g) were divided into four groups: Control, control + whey, MTX, and MTX+whey. MTX was administered at 20 mg/kg (single dose) intraperitoneally to the MTX group rats, and 2 mg/kg of whey protein were administered by oral gavage for 10 days to the whey groups. Lipid peroxidation, glutathione, and nitric oxide (NO) levels, as well as glutathione-Stransferase and superoxide dismutase activities were measured in the brain and small intestine. SDS-polyacrylamide gel electrophoresis of the brain and intestine tissues were also carried out. Results: While MTX treatment caused oxidative damage in the brain and small intestine, whey protein administration ameliorated MTXinduced oxidative stress. MTX administration did not change the brain's NO level, while an increase in intestinal NO level was detected. Conclusion: MTX induced oxidative stress in the brain and small intestine changed the protein metabolism in these tissues regardless of reduced food intake. Consecutive 10-day administration of whey proteins has shown its therapeutic effect on MTX-induced brain and small intestine oxidative damage.
Citation Count: 0
The Effects Of Panax Ginseng on Serum Oxidative Stress Following Bisphenol a Exposure
(Istanbul Univ, 2024) Ede Pazarbaşı, Seren; Şener, Göksel; Dorucu, Dogancan; Sener, Goksel; Tunali-Akbay, Tugba; Eczane Hizmetleri Bölümü; Eczacılık Meslek Bilimleri Bölümü
Objective: Bisphenol A (BPA) is a toxic compound that causes oxidative stress by disrupting antioxidant enzymes and promoting tissue lipid peroxidation. This study aimed to examine the impacts of BPA on serum oxidative stress in rats and to detect the antioxidant feature of Panax ginseng (PxG) in reducing BPA-induced oxidative stress. Materials and Methods: Wistar Albino rats (250-300 g) were divided into control, control + PxG, BPA, and BPA + PxG groups. 50 mg/kg BPA and 100 mg/g PxG were given for six weeks. Serum total antioxidant and oxidant status, lipid peroxidation, and glutathione levels were determined. Results: BPA administration increased total oxidant status and lipid peroxidation, while PxG administration to the BPA group decreased these parameters. PxG also increased total antioxidant status and glutathione levels compared to the BPA group. Conclusion: BPA was seen to cause an increase in oxidative parameters and PxG administration to restore the oxidative stress that was generated after BPA exposure, suggesting that this may help to prevent the adverse effects caused by BPA exposure.
Citation Count: 5
Inhibitory Effect of Whey Protein Concentrate on Sars-Cov Furin Activity and Spike Protein-Ace2 Binding in Methotrexate-Induced Lung Damage
(Wiley-hindawi, 2022) Yılmaz Karaoğlu, Sümeyye; Sivas, Guzin Goksun; Gurel-Gokmen, Begum; Yilmaz-Karaoglu, Sumeyye; Ercan, Dursun; Ozbeyli, Dilek; Tunali-Akbay, Tugba; Eczane Hizmetleri Bölümü
This study aims to investigate the effects of whey proteins on SARS CoV-2 in methotrexate-induced lung tissue damage in rats. To determine the possible effects, rats were divided into four groups as control, control + whey, methotrexate (20 mg/kg, i.p.) and methotrexate + whey. Whey protein concentrate (2 g/kg, oral gavage) was administered for 10 days. Cytokine levels were measured and protein electrophoresis was carried out in serum samples. Lipid peroxidation, nitric oxide and glutathione level, and superoxide dismutase and glutathione S transferase activities were determined in lung samples. Inhibition of SARS CoV-2-targeted lung furin activity and SARS CoV-2 spike protein-angiotensin converting enzyme binding with whey protein concentrate were also measured in each group. In conclusion, whey protein concentrate improved methotrexate-induced lung damage and inhibited lung furin activity targeting SARS-CoV-2 S1/S2 site cleavage and SARS CoV-2 spike protein-angiotensin converting enzyme binding. Whey proteins are potential protective candidates that inhibit SARS CoV-2-related interactions, even in methotrexate-induced lung injury. Practical applications Whey proteins have anticarcinogenic, antihypertensive, antioxidant, antibacterial, antiviral, and immunomodulating properties due to the protein, bioactive peptide, and essential amino acid content. Methotrexate is a folate antagonist and inhibits cell proliferation and purine synthesis. The combined use of whey protein concentrate and methotrexate may be an alternative in the development of new strategies to the treatment approaches against COVID-19. In addition, according to the results of this study, it is thought that the protective effect of whey proteins in healthy conditions before encountering the SARS CoV-2 may be higher than those who have never used it.
Citation Count: 7
Lactose Hydrolyzing Activity of the Lactase Immobilized Polycaprolactone and Silk Fibroin-Based Nanofiber and Nitrocellulose Membrane
(Elsevier, 2022) Yılmaz Karaoğlu, Sümeyye; Gurel-Gokmen, Beguem; Tunali-Akbay, Tugba; Eczane Hizmetleri Bölümü
This study aimed to investigate the activity of lactase immobilized polycaprolactone and silk fibroin (PCL/SF)-based nanofiber and nitrocellulose membrane for the preparation of lactose-reduced milk. PCL/SF-based nanofiber was prepared by using the electrospinning method. The lactase enzyme was immobilized using the physical adsorption method on both surfaces, and then the optimum operating temperature and pH of the immobilized enzymes were determined. The efficiency of the immobilized lactase enzyme was determined in both goat and cow milk. The nutrient content of milk was also analyzed before and after the incubation of nanofiber and membrane with milk. The lactose hydrolysis efficiency of the lactase immobilized nitrocellulose membrane was found to be higher than the lactase immobilized PCL/SF-based nanofiber. Lactose was hydro-lyzed 59% in cow milk and 87% in goat milk by using lactase immobilized nitrocellulose membrane. 42% of lactose was also hydrolyzed in cow milk and 21% was hydrolyzed in goat milk by using lactase immobilized PCL/ SF-based nanofiber. However, the use of these two bioactive surfaces did not change the fat and protein composition of both cow and goat milk. In conclusion, lactase immobilized nitrocellulose membrane was found to be more advantageous in the production of lactose-reduced milk than the lactase immobilized poly-caprolactone/silk fibroin nanofiber.
Citation Count: 4
Myrtle Improves Renovascular Hypertension-Induced Oxidative Damage in Heart, Kidney, and Aortic Tissue
(Springer, 2022) Şener, Göksel; Ozcan, Ozan; Gurel-Gokmen, Begum; Cevikelli-Yakut, Zatiye Ayca; Saygi, Halil Ibrahim; Sen, Ali; Tunali-Akbay, Tugba; Eczacılık Meslek Bilimleri Bölümü
Renovascular hypertension is defined as the reduction in renal perfusion resulting in sustained hypertension. This study aims to investigate the possible effects of myrtle leaf extract (Myrtus communis L.) on, heart, kidney and aorta tissues in the experimental renovascular hypertension (RVH). 32 male Wistar Albino rats were divided into four groups as control, hypertension, hypertension+ramipril, and hypertension+myrtle leaf extract treatment groups. RVH model was induced by Goldblatt's 2-kidney 1-clip method. 12 weeks later than the treatment blood pressures were recorded and oxidant and antioxidant parameters, tissue factor activity, and histological analysis were determined in the kidney, heart, and aortic tissues. The blood pressure levels of the hypertension group significantly increased compared to the control group. Administration of myrtle leaf extract and ramipril significantly decreased the increased blood pressure. In the hypertension group, oxidative damage increased in the kidney, heart, and aorta tissues. In the histological evaluation of tissues in RVH, heart muscle fibres degenerated. Bowman capsule and glomeruli dilated and tubules damaged in the kidney. Myrtle leaf extract administration regenerated the damages and degenerations. The administration of myrtle leaf extract restored the impaired oxidant-antioxidant balance in the heart, kidney and aorta tissues of hypertensive rats. Myrtle leaf extract can be considered as an alternative antihypertensive treatment target in the prevention of oxidative stress-induced damage in renovascular hypertension.
Citation Count: 0
Panax Ginseng Extract Ameliorates Methotrexate-Induced Multi-Organ Damage Via the Regulation of Oxidative Stress
(Marmara Univ, 2023) Ede Pazarbaşı, Seren; Yılmaz Karaoğlu, Sümeyye; Şener, Göksel; Tunali-Akbay, Tugba; Karakaya-Cimen, Fatma Bedia; Ercan, Feriha; Sener, Goksel; Eczane Hizmetleri Bölümü; Eczacılık Meslek Bilimleri Bölümü
Oxidative damage plays an important role in organ toxicities caused by methotrexate (MTX). This study aimed to determine the antioxidant effects of Panax ginseng (PxG) extract against MTX-induced liver, lung, ileum and kidney damage. Twenty-four Sprague Dawley male rats (weight 250-300 g) were used in the study. The animals were randomly separated into three groups: a) Control, b) MTX-treated (MTX) and c) MTX+PxG-treated (MTX+PxG) groups. MTX was administered intraperitoneally at 20 mg/kg, as a single dose to MTX and MTX+PxG groups. PxG was administered orally at 100 mg/kg to the MTX+PxG group for five days. Saline was given to the control and MTX groups for 5 days. At the end of the experiment, liver, lung, ileum, and kidney samples were obtained. Malondialdehyde (MDA) and glutathione (GSH) levels, superoxide dismutase (SOD), glutathione-S-transferase (GST) and tissue factor (TF) activities were determined in all tissues. In addition, histological examinations were done through light microscopy. GraphPad Prism 5v. was used for statistics, and p<0.05 were considered significant. Administration of MTX caused severe injury in tissues. Findings showed that MDA level, SOD, and GST activities were significantly normalized in the MTX+PxG group compared to the control group. A significant reduction in GSH level observed in the MTX group was reversed with PxG administration In addition, TF activity and total protein levels were found to be impaired in the MTX group, but TF activity was significantly improved in liver and lung tissues and total protein level was significantly reversed in lung and ileum in MTX+PxG group. The results of histological examinations showed that MTX-induced damage was ameliorated with the PxG administration. In conclusion, this study shows that Panax ginseng, thanks to its antioxidant properties, reversed MTX-induced tissue damage and therefore may be beneficial against side effects in patients undergoing chemotherapy.
Citation Count: 2
Whey Protein Concentrate Ameliorates the Methotrexate-Induced Liver and Kidney Damage
(Cambridge Univ Press, 2023) Yılmaz Karaoğlu, Sümeyye; Sivas, Guezin Goksun; Gurel-Gokmen, Begum; Yilmaz-Karaoglu, Suemeyye; Dursun, Ercan; Caliskan-Ak, Esin; Tunali-Akbay, Tugba; Eczane Hizmetleri Bölümü
Methotrexate (MTX) is a cytotoxic immunosuppressant that is widely used in the treatment of tumours, rheumatoid arthritis and psoriasis. This study aims to evaluate the effects of whey proteins on MTX-induced liver and kidney damage by focusing on oxidant-antioxidant systems and eating habits. The study was conducted in four groups of thirty Sprague-Dawley rats (control, control + whey protein concentrate (WPC), MTX, MTX + WPC). A single dose of 20 mg/kg MTX was administered intraperitoneally to the MTX groups. Control and MTX groups were given 2 g/kg WPC by oral gavage every day for 10 d. At the end of day 10, blood samples were drawn and liver and kidney tissues were removed. MTX administration increased the lipid peroxidation level and decreased glutathione level, superoxide dismutase and glutathione-S-transferase activities in the liver and kidney. Administration of WPC significantly reduced the damage caused by MTX in the liver and kidney. While a decrease in serum urea level and an increase in serum creatinine level were detected in the MTX group, WPC administration reversed these results up to control group levels. Administration of WPC to the MTX group significantly reversed the histopathological damage scores of the liver and kidney. WPC administration ameliorated the MTX-induced oxidative damage in the liver and kidney tissues due to its antioxidant properties. Liver and kidney damage can be prevented by using whey proteins as a nutraceutical in MTX therapy. In conclusion, whey proteins demonstrated a protective effect against MTX-induced liver and kidney damage.