Browsing by Author "Emekli-Alturfan, Ebru"

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Biocompatibility Assessment of Chlorhexidine Gluconate Versus a Natural Mouthwash Containing Olea Europaea and Opuntia Ficus-Indica in Zebrafish Embryos
(2025) Ünal, İsmail; Emekli-Alturfan, Ebru; Cansız, Derya; Yalcinkaya, Sebnem Ercalik; Beler, Merih; Eğilmezer, Gizem; Karagöz, Atakan
Various chemical solutions are available for oral hygiene and care, but these chemical solutions also cause various side effects in living systems. Therefore, the trend towards the production of highly biocompatible, natural-based oral care products has increased. This study aimed to compare the biocompatibility profiles of a conventional chlorhexidine gluconate-based mouthwash and a natural formulation containing Olea europaea leaf and Opuntia ficus-indica extracts using the zebrafish embryo model. Zebrafish embryos were exposed to two concentrations (100 ppm and 1000 ppm) of each mouthwash for 72 hours post-fertilization. Developmental properties such as mortality, hatching rate, pericardial edema, and body length were evaluated. Biochemical analyses included oxidative stress parameters and acetylcholinesterase (AChE) activity. Chlorhexidine exposure resulted in increased embryonic mortality, pericardial edema, and reduced body length. Biochemically, chlorhexidine increased lipid peroxidation and glutathione S-transferase (GST) activity while decreasing superoxide dismutase (SOD) and AChE activities. In contrast, embryos exposed to the natural formulation showed no significant developmental abnormalities and exhibited increased SOD and AChE activities without changes in lipid peroxidation. These findings provide evidence regarding the differential biocompatibility of synthetic and plant-based mouthwashes in early developmental models.
Brain in Metabolic Syndrome Model: the Effect of Exercises and Caloric Restriction
(Marmara Univ, 2022) Alev-Tuzuner, Burcin; Genc-Kahraman, Nevin; Ipekci, Hazal; Ustundag, Unsal Veli; Tunali-Akbay, Tugba; Emekli-Alturfan, Ebru; Yarat, Aysen
Caloric restriction (CR) and exercise (EX) have impacts on improving metabolic risk factors. This study aimed to investigate the changes in the brain after EX and/or CR in metabolic syndrome (MeS) induced by a high fructose diet in rats. Sprague-Dawley male rats were divided into five groups. Drinking water including 10% fructose solution was given to rats for 12 weeks to develop a MeS rat model. Animals with MeS were submitted to EX and/or CR for 6 weeks. Blood glucose, and brain tissue damage and antioxidant parameters were measured. Brain lipid peroxidation, sialic acid, mucin, fucose levels increased in the MeS group compared to the control (C) group. These parameters reduced significantly in the metabolic syndrome with caloric restriction (MeS+CR) group, and more significantly in the metabolic syndrome with exercise and caloric restriction group (MeS+EXCR), compared to the MeS group. Glutathione levels, superoxide dismutase and catalase activities decreased in the MeS group compared to the C group, increased both in the MeS+CR group, and MeS+EXCR group compared to the MeS group. High fructose diet consumption can lead to brain tissue damage and decreased antioxidant levels were found to be improved best in the MeS+EXCR group.
Toothpastes with Different Detergent Contents Affect Morphogenesis and Redox System Parameters in Zebrafish Embryos
(Wiley, 2025) Karagoz, Atakan; Beler, Merih; Egilmezer, Gizem; Unal, Ismail; Cansiz, Derya; Emekli-Alturfan, Ebru