Browsing by Author "Bardakci, Hilal"

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Evaluation of Antioxidant, Anti-Inflammatory and Neuroprotective Activities and Chemical Contents of Species Used Traditionally for Inflammation in Turkey
(Georg Thieme verlag Kg, 2023) Sen, Burcu; Ilhan, Sukran-Kubra; Erel, Buge; Gul, Zulfiye; Avsar, Timucin; Bardakci, Hilal; Diesel, Britta; Eczacılık Meslek Bilimleri Bölümü
[No Abstract Available]
Citation - WoS: 10
in Vitro Assessment of Dermatological Activity Potential Of Achillea Clypeolata Sm. in H2o2-Treated Human Dermal Fibroblasts
(Elsevier, 2023) Barak, Timur Hakan; Kurt-Celep, Inci; Dilek-Tepe, Hafize; Bardakci, Hilal; Akaydin, Galip; Yesilada, Erdem; Celep, Engin; Eczacılık Meslek Bilimleri Bölümü
Members of Achilllea L. genus are widely used against dermatological disorders in traditional medicine. An increasing number of experimental studies indicated that these species are an important source of ailments against such conditions. Besides, clinical studies yield supportive results about the dermatological effects of the genus. However, the number of studies stating the mechanisms of actions is quite scarce. A. clypeolata Sn. grows naturally in the Thrace region of Turkiye, and is used against various health problems in folk medicine. The methanolic extract prepared from the aerial parts of the plant (ACM) was subjected to a series of tests focusing on the mechanisms of dermatological activity. First, in vitro antioxidant screening tests, including DPPH, CUPRAC, FRAP and TOAC were applied. Then, the inhibitory potential of ACM against skin-related enzymes such as collagenase, elastase and hyaluronidase was measured. For a more detailed profiling of the activity, human dermal fibroblast cells (HDFs) treated with H2O2 were given varying concentrations of ACM. WST-1 analysis was carried out for the assessment of cell viability, and DCFDA (20,70-dichlorofluorescin diacetate) method was employed for the measurement of cellular antioxidant activity. In addition, the inhibitory potentials of ACM against matrix metalloproteinase-2 and-9 enzymes, which are responsible for the degradation of extracellular matrix components in the skin, were also tested. The total phenolic (26.02 & PLUSMN; 1.04 mg GAE/g dry extract) and flavonoid (12.85 & PLUSMN; 0.69 mg QE/g dry extract) contents of ACM were calculated with the activity tests. Also, for a comprehensive quantitative analysis, LC-MS/MS analysis was carried out. All of the data gained from the mentioned tests and analysis revealed the high capacity of ACM in dermatological conditions such as the inhibitory potential enzymes associated with skin aging. Phytochemical profiling studies showed that the extract is rich in phenolic compounds, specifically chlorogenic acid, hesperidin and hyperoside. Data obtained from this study suggest that A. clypeolata may act as a natural source of skin-active ingredients. & COPY; 2023 SAAB. Published by Elsevier B.V. All rights reserved.
In Vitro Efficacy of Sumac (Rhus coriaria) Extracts Against Leishmania Tropicana and Leishmania Mexicana: A Preliminary Study from Turkiye
(Istanbul Univ, 2025) Mete, Ergun; Ozel, Yener; Bardakci, Hilal; Durmuskahya, Cenk; Koseler, Aylin; Kurt, Ozgur; Eczacılık Meslek Bilimleri Bölümü
Objective: Cutaneous leishmaniasis (CL) is a common clinical manifestation of leishmaniasis. Here, the in vitro anti-leishmanial efficacy of sumac extracts was tested for the first time on both Leishmania (L.) tropica and L. mexicana isolates using Rhus (R.) coriaria plant, which was collected in western Anatolia. Materials and Methods: The dried and powdered fruits of R. coriaria were macerated in acetone, ethyl alcohol, and ethyl alcohol-water mixture at room temperature for two days. The pooled extracts were evaporated under reduced pressure and lyophilized form for the study. Isolates of L. tropica and L. mexicana in Acibadem University R&D Laboratory were initially thawed and cultivated in NNN medium. Assessments were made using the haemocytometer and MTT methods at 24 and 48 h, compared with meglumine antimoniate as the control group. Results: For L. tropica, the effective concentration ranges of the extracts and the infusion were found to be 578.13-289.06 pg/mL and 289.06-144.53 pg/mL, respectively. For L. mexicana, the ranges were found to be 289.06-144.53 pg/mL and 144.53-72.27 pg/mL, respectively. It was shown that all extracts of R. coriaria were effective against both L. tropica and L. mexicana in higher doses, compared to meglumine antimoniate. Conclusion: An interesting finding was that higher sumac doses were required to eliminate L. tropica of the Old World, compared to L. mexicana of the New World. In addition, the aqueous alcohol extract showed efficacy that lasted for 48 h in half doses compared to others in L. tropica. Further assessments for both the identification of the active compounds within R. coriaria and their efficacy in vivo are planned.