Browsing by Author "Barak, Timur Hakan"

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A Comparative Study of Biochemical, Antimicrobial Effects and Phytochemical Composition Analysis of Glycyrrhiza Glabra L. Varieties Root Extracts
(Marmara Univ, Fac Pharmacy, 2025) Gulmez, Gizem; Sen, Ali; Servi, Huseyin; Barak, Timur Hakan; Tekin, Fetullah; Marzi, Mahdi; Sener, Azize
Plants are the significant global interest as alternative treatment sources with their biologically active compounds. This study compares the chemical composition and the antioxidant, antidiabetic, and antimicrobial properties of ethanol extracts of G. glabra L. two different varieties from different regions. The phytochemical compositions was determined using GC-MS. Additionaly, total phenolic (TPC), flavonoid (TFC) and triterpene (TTC) contents were determined. Glycyrrhizic acid contents were analysed by HPLC. G. glabra var. glandulifera (GF1) showed the highest antioxidant activity. All extracts had strong antidiabetic effects, besides GF1 showing the highest effect. The MIC values was determined against 8 bacterial and 1 yeast strain and values ranged from 2.500 to 0.500; 2.500 to 0.714; 2.500 to 0.714 for G. glabra var. glabra (GB), GF1, G. glabra var. glandulifera (GF2) respectively. Phytochemical studies have shown that TPC was 100.60 +/- 5.06, 127.90 +/- 0.30, 69.01 +/- 0.30 mg GAE /g extract; TFC was 80.07 +/- 0.15, 25.35 +/- 0.0, 16.58 +/- 0.31 mg KE/g and TTC was 217.30 +/- 6.05,172.40 +/- 2.17, 126.30 +/- 4.50 mg OE/g extract for GB, GF1, GF2, respectively. GF1 in particular has the highest glycyrrhizic acid content. This study will contribute to the creation of new treatment strategies and potential therapeutic agents in addition to the use of G. glabra L. in traditional treatments. Our study is also a preliminary study for future studies.
Citation - WoS: 12
in Vitro Assessment of Dermatological Activity Potential Of Achillea Clypeolata Sm. in H2o2-Treated Human Dermal Fibroblasts
(Elsevier, 2023) Barak, Timur Hakan; Kurt-Celep, Inci; Dilek-Tepe, Hafize; Bardakci, Hilal; Akaydin, Galip; Yesilada, Erdem; Celep, Engin; Eczacılık Meslek Bilimleri Bölümü
Members of Achilllea L. genus are widely used against dermatological disorders in traditional medicine. An increasing number of experimental studies indicated that these species are an important source of ailments against such conditions. Besides, clinical studies yield supportive results about the dermatological effects of the genus. However, the number of studies stating the mechanisms of actions is quite scarce. A. clypeolata Sn. grows naturally in the Thrace region of Turkiye, and is used against various health problems in folk medicine. The methanolic extract prepared from the aerial parts of the plant (ACM) was subjected to a series of tests focusing on the mechanisms of dermatological activity. First, in vitro antioxidant screening tests, including DPPH, CUPRAC, FRAP and TOAC were applied. Then, the inhibitory potential of ACM against skin-related enzymes such as collagenase, elastase and hyaluronidase was measured. For a more detailed profiling of the activity, human dermal fibroblast cells (HDFs) treated with H2O2 were given varying concentrations of ACM. WST-1 analysis was carried out for the assessment of cell viability, and DCFDA (20,70-dichlorofluorescin diacetate) method was employed for the measurement of cellular antioxidant activity. In addition, the inhibitory potentials of ACM against matrix metalloproteinase-2 and-9 enzymes, which are responsible for the degradation of extracellular matrix components in the skin, were also tested. The total phenolic (26.02 & PLUSMN; 1.04 mg GAE/g dry extract) and flavonoid (12.85 & PLUSMN; 0.69 mg QE/g dry extract) contents of ACM were calculated with the activity tests. Also, for a comprehensive quantitative analysis, LC-MS/MS analysis was carried out. All of the data gained from the mentioned tests and analysis revealed the high capacity of ACM in dermatological conditions such as the inhibitory potential enzymes associated with skin aging. Phytochemical profiling studies showed that the extract is rich in phenolic compounds, specifically chlorogenic acid, hesperidin and hyperoside. Data obtained from this study suggest that A. clypeolata may act as a natural source of skin-active ingredients. & COPY; 2023 SAAB. Published by Elsevier B.V. All rights reserved.