Browsing by Author "Aykac, Asli"

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Citation Count: 1
A Comprehensive Assessment of the Cholinergic-Supporting and Cognitive-Enhancing Effects of rosa Damascena Mill. (damask Rose) Essential Oil on Scopolamine-Induced Amnestic Rats
(Wiley, 2024) Şener, Göksel; Ozbeyli, Dilek; Yigit-Hanoglu, Duygu; Baser, Kemal Husnu Can; Sener, Goksel; Aykac, Asli; Eczacılık Meslek Bilimleri Bölümü
Introduction: Alzheimer's disease (AD) is a neurodegenerative condition characterized by gradual loss of cognitive abilities (dementia) and is a major public health problem. Here, we aimed at investigating the effects of Rosa damascena essential oil (RDEO) on learning and memory functions in a rat model of amnesia induced by scopolamine, as well as on changes in acetylcholinesterase (AChE) activity, M-1 muscarinic acetylcholine receptor (mAChR) expression, and brain-derived neurotrophic factor (BDNF) levels in the extracted brain tissues. Methods: The control, amnesia (scopolamine, 1 mg/kg/i.p.) and treatment (RDEO, 100 mu L/kg/p.o. or galantamine, 1.5 mg/kg/i.p.) groups were subjected to Morris water maze and new object recognition tests. AChE activity was assayed by ELISA, and M-1 mAChR and BDNF concentration changes were determined by western blotting. Also, using computational tools, human M-1 mAChR was modeled in an active conformation, and the major components of RDEO were docked onto this receptor. Results: According to our behavioral tests, RDEO was able to mitigate the learning and memory impairments caused by scopolamine in vivo. Our in vitro assays showed that the observed positive effects correlated well with a decrease in AChE activity and an increase in M-1 mAChR and BDNF levels in amnestic rat brains. We also demonstrated in an in silico setting that the major components of RDEO, specifically -citronellol, geraniol, and nerol, could be accommodated favorably within the allosteric binding pocket of active-state human M-1 mAChR and anchored here chiefly by hydrogen-bonding and alkyl-pi interactions. Conclusion: Our findings offer a solid experimental foundation for future RDEO-based medicinal product development for patients suffering from AD.
Citation Count: 11
petroselinum Crispum Extract Ameliorates Scopolamine-Induced Cognitive Dysfunction: Role on Apoptosis, Inflammation and Oxidative Stress
(Tsinghua Univ Press, 2022) Şener, Göksel; Ede Pazarbaşı, Seren; Ozbeyli, Dilek; Ede, Seren; Yanardag, Refiye; Sacan, Ozlem; Aykac, Asli; Eczacılık Meslek Bilimleri Bölümü; Eczane Hizmetleri Bölümü
This study was designed to investigate whether Petroselinum crispum (PC) extract has protective effects on the brain in the scopolamine-induced Alzheimer's disease (AD) rut model. The rats were divided into; control, scopolamine (1 mg/kg, i.p.), galantamine (1.5 mg/kg, i.p.) and PC extract (2 g/kg, p.o.)-treated scopolamine groups. On day 14, the novel object recognition test (NORT) and Morris water maze test (MWMT) were performed and then the rats were sacrificed. Scopolamine-induced cognitive impairments observed in the NORT and MWMT, significantly improved with PC extract and galantamine treatments. Scopolamine reduced M, receptor expression, Bcl-2/Bax ratio, and glutathione levels in the hippocampus and frontal cortex, while malondialdehyde levels, caspase-3/9 expressions, and acetylcholinesterase (AChE) activity were found to be increased. On the other band, PC and galantamine treatments reversed these changes. In conclusion, PC extract has shown an ameliorative effect on the spatial and recognition memory, M-1 receptor expression, apoptosis, oxidative stress, and increased AChE activity. Thus, it was concluded that PC could prevent AD-like conditions and can be used as a functional food. However, since animal models do not completely mimic those of humans, based on the data obtained in this study, the importance of PC on human AD should be demonstrated in future studies. (C) 2022 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.
Citation Count: 5
A Multi-Parameter Evaluation of the Neuroprotective and Cognitive-Enhancing Effects of origanum Onites L. (turkish Oregano) Essential Oil on Scopolamine-Induced Amnestic Rats
(Springer/plenum Publishers, 2022) Ede Pazarbaşı, Seren; Şener, Göksel; Ozbeyli, Dilek; Ede, Seren; Albayrak, Omercan; Baser, Kemal Husnu Can; Sener, Goksel; Eczane Hizmetleri Bölümü; Eczacılık Meslek Bilimleri Bölümü
Alzheimer's disease (AD) is a neurodegenerative disorder characterized by progressive deterioration of cognitive functions (dementia) and represents a growing public health concern since the population in the age groups at risk is increasing. The latter raises an urgent need to translate research findings in the basic brain and behavioral sciences into anti-AD drugs and disease-modifying therapies. Origanum onites (L.), also called Turkish oregano, is a perennial and herbaceous plant species grown for centuries for medicinal, cosmetic and culinary purposes. This is the first study to investigate the putative neuroprotective and pro-cognitive activities of O. onites essential oil (OOEO) against scopolamine-induced amnesia of AD-type in Wistar albino rats. The results of behavioral tests revealed that OOEO administration was able to significantly alleviate learning and memory impairments induced by scopolamine in vivo. The observed effects could be attributed to inhibition of acetylcholinesterase activity, attenuation of oxidative stress and prevention of neuronal apoptosis in the hippocampus and frontal cortex of AD rats. Modulation of pro-inflammatory enzymes, including cyclooxygenase-2, inducible nitric oxide synthase and myeloperoxidase, might further contribute to the neuroprotective properties of OEOO, as predicted by our in silico models. These findings offer novel insights into the therapeutic potential of OEOO in patients with AD.
Citation Count: 0
A Multi-Parameter Evaluation of the Neuroprotective and Cognitive-Enhancing Effects of Origanum Onites L. (turkish Oregano) Essential Oil on Scopolamine-Induced Amnestic Rats (feb,10.1007/S11011-022-00933-6, 2022)
(Springer/plenum Publishers, 2022) Ede Pazarbaşı, Seren; Şener, Göksel; Ozbeyli, Dilek; Ede, Seren; Albayrak, Omercan; Baser, Kemal Husnu Can; Sener, Goksel; Eczane Hizmetleri Bölümü; Eczacılık Meslek Bilimleri Bölümü
[No Abstract Available]