Küçükgüzel, Şükriye GünizKoc, Hande CevherAtlihan, IremMega-Tiber, PinarOrun, OyaKucukguzel, S. GunizEczacılık Meslek Bilimleri Bölümü2025-01-112025-01-112022112630-634410.29228/jrp.972-s2.0-85121980927https://doi.org/10.29228/jrp.97https://hdl.handle.net/20.500.14627/114Mega Tiber, Pinar/0000-0003-0819-0702; Atlihan, Irem/0000-0003-0351-0279(R,S)-Etodolac [1,8-diethyl-1,3,4,9-tetrahydrapyrano(3,4-b)indole-1-acetic acid] is a nonsteroidal anti-inflammatory drug that contains carboxylic acid group with the structure of pyrano[3,4-h]indole. In this study, a series of novel (R,S)-Etodolac derivatives (3a-1) bearing hydrazide-hydrazone moiety were synthesized. The structures of these compounds were characterized by spectral (H-1-NMR and FT-IR analyses) methods. All synthesized compounds were screened for anticancer activity against androgen-independent prostate adenocarcinoma (PC-3, DU-145) and androgen-dependent prostate adenocarcinoma (LNCaP) cell lines by using WST-8 colorimetric method. This method was used for cell viability and cytotoxicity analysis. Compound 3b (SGK-720) [2-(1,8-diethyl-1,3,4,9-tetrahydropyrano[3,4-b]indole-1-yl)acetic acid[(2,6-dichlorophenyl)methylene]hydrazides] showed 10.36, 5.24, 15.53 mu M anticancer activity against PC3, DU145, LNCaP cancer cell lines, respectively. According to JC-1 mitochondrial membrane potential test and Annexin V/PI staining, 3b was found to have apoptotic effect on these cancer cells. It is concluded that compound 3b containing 2,6-dichloro substituents may be one of the candidate molecules to cope with prostate cancer.eninfo:eu-repo/semantics/openAccessEtodolacHydrazide-HydrazoneAnticancer ActivityApoptosisWst-8 Colorimetric MethodArticleQ4261112WOS:000738259100001520427