Küçükgüzel, Şükriye GünizSenkardes, SevilErdogan, OmerCevik, OzgeKucukguzel, S. GunizEczacılık Meslek Bilimleri Bölümü2025-01-112025-01-11202190039-79111532-243210.1080/00397911.2021.19451052-s2.0-85109791511https://doi.org/10.1080/00397911.2021.1945105https://hdl.handle.net/20.500.14627/202erdogan, omer/0000-0002-8327-7077; senkardes, sevil/0000-0002-0523-459X; Cevik, Ozge/0000-0002-9325-3757In our continuing search for new anticancer agents, herein we report the synthesis of 2-(4-chloro-3-methylphenoxy)-N'-[(aryl)methylidene]acetohydrazides 3a-j and the evaluation of their anticancer activities on cell viability, morphological changes and caspase-3 activity in cancer cell lines including gastric cancer (MKN45), cervical cancer (HeLa) and breast cancer (MDA-MB-231) cells. 2-(4-chloro-3-methylphenoxy)-N'-[(4-phenylthiophen-2-yl)methylidene] acetohydrazide 3g presented the strongest growth inhibition against MKN45 gastric cancer cell lines with the IC50 value of 1.471 +/- 0.23 mu M. Moreover, compounds 3b and 3g showed high potency against the HeLa and MDA-MB-231 cell lines having IC50 in the range of 2.38-9.72 mu M. These compounds are more selective for the tested human cancer cells than for the mouse fibroblast cell line (NIH/3T3). As a result of the studies conducted in order to understand the molecular mechanism, compounds 3b and 3g enhanced expression of the caspase-3 pro-apoptotic proteins levels besides caspase-3 gene.eninfo:eu-repo/semantics/openAccessAnticancer ActivityCaspase-3CresolCytotoxicityHydrazoneSynthesisArticleQ3Q2511726342643WOS:000669741400001